0	2	RB	RB	NNP
3	6	and	and	CC
7	8	a	a	DT
9	14	novel	novel	JJ
15	20	E2F-1	e2f-1	NN
21	28	binding	binding	NN
29	36	protein	protein	NN
37	39	in	in	IN
40	43	MHC	MHC	NNP
44	49	class	class	NN
50	52	II	ii	CD
53	62	deficient	deficient	JJ
63	69	B-cell	b-cell	NN
70	75	lines	line	NNS
76	79	and	and	CC
80	86	normal	normal	JJ
87	96	IFN-gamma	IFN-gamma	NNP
97	106	induction	induction	NN
107	109	of	of	IN
110	113	the	the	DT
114	119	class	class	NN
120	122	IL	IL	NNP
123	137	transactivator	transactivator	NN
138	143	CIITA	CIITA	NNP
144	146	in	in	IN
147	152	class	class	NN
153	155	II	ii	CD
156	169	non-inducible	non-inducible	JJ
170	182	RB-defective	rb-defective	JJ
183	188	tumor	tumor	NN
189	194	lines	line	NNS
194	195	.	.	.

197	200	The	the	DT
201	206	major	major	JJ
207	225	histocompatibility	histocompatibility	NN
226	227	(	(	(
227	230	MHC	MHC	NNP
230	231	)	)	)
232	237	class	class	NN
238	240	II	ii	CD
241	246	genes	gene	NNS
247	253	encode	encode	VBP
254	258	cell	cell	NN
259	266	surface	surface	NN
267	275	proteins	protein	NNS
276	280	that	that	WDT
281	285	bind	bind	VBP
286	295	antigenic	antigenic	JJ
296	303	peptide	peptide	NN
304	307	for	for	IN
308	320	presentation	presentation	NN
321	323	to	to	TO
324	331	T-cells	T-cell	NNP
331	332	.	.	.

333	336	The	the	DT
337	342	class	class	NN
343	345	II	ii	CD
346	354	proteins	protein	NNS
355	358	are	be	VBP
359	368	expressed	express	VBN
369	383	constitutively	constitutively	RB
384	386	on	on	IN
387	394	B-cells	B-cell	NNP
395	398	and	and	CC
399	414	EBV-transformed	ebv-transformed	JJ
415	422	B-cells	B-cell	NNP
422	423	,	,	,
424	427	and	and	CC
428	431	are	be	VBP
432	441	inducible	inducible	JJ
442	444	by	by	IN
445	454	IFN-gamma	IFN-gamma	NNP
455	457	on	on	IN
458	459	a	a	DT
460	464	wide	wide	JJ
465	472	variety	variety	NN
473	475	of	of	IN
476	480	cell	cell	NN
481	486	types	type	NNS
486	487	.	.	.

488	502	Retinoblastoma	retinoblastoma	NN
503	510	protein	protein	NN
511	512	(	(	(
512	514	RB	RB	NNP
514	515	)	)	)
516	518	is	be	VBZ
519	520	a	a	DT
521	526	tumor	tumor	NN
527	537	suppressor	suppressor	NN
538	541	and	and	CC
542	551	functions	function	VBZ
552	554	as	as	IN
555	556	a	a	DT
557	572	transcriptional	transcriptional	JJ
573	582	repressor	repressor	NN
583	585	by	by	IN
586	593	binding	bind	VBG
594	597	and	and	CC
598	610	inactivating	inactivate	VBG
611	614	the	the	DT
615	629	transactivator	transactivator	NN
630	635	E2F-I	E2F-I	NNP
635	636	.	.	.

637	649	RB-defective	rb-defective	JJ
650	655	tumor	tumor	NN
656	661	lines	line	NNS
662	665	are	be	VBP
666	679	non-inducible	non-inducible	JJ
680	683	for	for	IN
684	687	MHC	MHC	NNP
688	693	class	class	NN
694	696	II	ii	CD
697	699	by	by	IN
700	709	IFN-gamma	IFN-gamma	NNP
709	710	,	,	,
711	713	or	or	CC
714	718	very	very	RB
719	725	weakly	weakly	RB
726	735	inducible	inducible	JJ
735	736	,	,	,
737	740	but	but	CC
741	753	transfection	transfection	NN
754	756	of	of	IN
757	758	2	2	CD
759	768	different	different	JJ
769	774	lines	line	NNS
775	779	with	with	IN
780	782	RB	RB	NNP
783	793	expression	expression	NN
794	801	vectors	vector	NNS
802	816	re-establishes	re-establishe	VBZ
817	819	or	or	CC
820	833	substantially	substantially	RB
834	842	enhances	enhance	VBZ
843	848	class	class	NN
849	851	II	ii	CD
852	864	inducibility	inducibility	NN
864	865	.	.	.

866	875	Therefore	therefore	RB
875	876	,	,	,
877	879	we	we	PRP
880	888	examined	examine	VBD
889	892	the	the	DT
893	895	RB	RB	NNP
896	902	status	status	NN
903	905	of	of	IN
906	907	a	a	DT
908	914	series	series	NN
915	917	of	of	IN
918	924	B-cell	b-cell	NN
925	932	mutants	mutant	NNS
933	937	that	that	WDT
938	941	are	be	VBP
942	951	defective	defective	JJ
952	954	in	in	IN
955	960	class	class	NN
961	963	II	ii	CD
964	974	expression	expression	NN
974	975	,	,	,
976	985	generated	generate	VBN
986	992	either	either	CC
993	995	in	in	FW
996	1001	vitro	vitro	FW
1002	1004	or	or	CC
1005	1012	derived	derive	VBN
1013	1017	from	from	IN
1018	1022	Bare	Bare	NNP
1023	1033	Lymphocyte	Lymphocyte	NNP
1034	1042	Syndrome	Syndrome	NNP
1043	1044	(	(	(
1044	1047	BLS	BLS	NNP
1047	1048	)	)	)
1049	1057	patients	patient	NNS
1057	1058	.	.	.

1059	1066	Nuclear	nuclear	JJ
1067	1079	matrix-bound	matrix-bound	JJ
1080	1082	RB	rb	NN
1083	1086	was	be	VBD
1087	1097	detectable	detectable	JJ
1098	1100	in	in	IN
1101	1104	all	all	DT
1105	1110	cases	case	NNS
1110	1111	,	,	,
1112	1122	indicating	indicate	VBG
1123	1127	that	that	IN
1128	1132	loss	loss	NN
1133	1135	of	of	IN
1136	1138	RB	RB	NNP
1139	1141	is	be	VBZ
1142	1145	not	not	RB
1146	1157	responsible	responsible	JJ
1158	1161	for	for	IN
1162	1171	decreased	decrease	VBN
1172	1177	class	class	NN
1178	1180	II	ii	CD
1181	1191	expression	expression	NN
1192	1194	in	in	IN
1195	1200	these	these	DT
1201	1206	lines	line	NNS
1206	1207	.	.	.

1208	1209	A	a	DT
1210	1216	second	second	JJ
1217	1222	E2F-I	E2F-I	NNP
1223	1230	binding	binding	NN
1231	1238	protein	protein	NN
1238	1239	,	,	,
1240	1244	most	most	RBS
1245	1251	likely	likely	RB
1252	1256	DP-I	DP-I	NNP
1256	1257	,	,	,
1258	1261	was	be	VBD
1262	1266	also	also	RB
1267	1277	apparently	apparently	RB
1278	1284	normal	normal	JJ
1285	1287	in	in	IN
1288	1292	both	both	CC
1293	1298	class	class	NN
1299	1310	II-positive	ii-positive	JJ
1311	1314	and	and	CC
1315	1324	-negative	-negative	JJ
1325	1331	B-cell	b-cell	NN
1332	1337	lines	line	NNS
1337	1338	.	.	.

1339	1341	We	we	PRP
1342	1346	also	also	RB
1347	1355	examined	examine	VBD
1356	1359	the	the	DT
1360	1369	IFN-gamma	IFN-gamma	NNP
1370	1379	induction	induction	NN
1380	1382	of	of	IN
1383	1388	CIITA	CIITA	NNP
1389	1391	in	in	IN
1392	1404	RB-defective	rb-defective	JJ
1405	1410	lines	line	NNS
1410	1411	.	.	.

1412	1417	CIITA	CIITA	NNP
1418	1420	is	be	VBZ
1421	1422	a	a	DT
1423	1428	class	class	NN
1429	1431	II	ii	CD
1432	1436	gene	gene	NN
1437	1451	transactivator	transactivator	NN
1452	1457	known	know	VBN
1458	1460	to	to	TO
1461	1463	be	be	VB
1464	1473	defective	defective	JJ
1474	1476	in	in	IN
1477	1480	one	one	CD
1481	1485	form	form	NN
1486	1488	of	of	IN
1489	1492	BLS	bls	NN
1493	1496	and	and	CC
1497	1499	to	to	TO
1500	1502	be	be	VB
1503	1511	required	require	VBN
1512	1515	for	for	IN
1516	1519	the	the	DT
1520	1529	induction	induction	NN
1530	1532	of	of	IN
1533	1536	MHC	MHC	NNP
1537	1542	class	class	NN
1543	1545	II	ii	CD
1546	1548	by	by	IN
1549	1558	IFN-gamma	IFN-gamma	NNP
1558	1559	.	.	.

1560	1565	CIITA	CIITA	NNP
1566	1570	mRNA	mRNA	NNP
1571	1573	is	be	VBZ
1574	1582	normally	normally	RB
1583	1592	inducible	inducible	JJ
1593	1595	by	by	IN
1596	1605	IFN-gamma	IFN-gamma	NNP
1606	1608	in	in	IN
1609	1614	class	class	NN
1615	1617	II	ii	CD
1618	1631	non-inducible	non-inducible	JJ
1631	1632	,	,	,
1633	1645	RB-defective	rb-defective	JJ
1646	1651	lines	line	NNS
1651	1652	,	,	,
1653	1656	and	and	CC
1657	1659	in	in	IN
1660	1663	one	one	CD
1664	1668	line	line	NN
1668	1669	,	,	,
1670	1683	re-expression	re-expression	NN
1684	1686	of	of	IN
1687	1689	RB	RB	NNP
1690	1693	has	have	VBZ
1694	1696	no	no	DT
1697	1703	effect	effect	NN
1704	1706	on	on	IN
1707	1712	CIITA	CIITA	NNP
1713	1717	mRNA	mRNA	NNP
1718	1727	induction	induction	NN
1728	1734	levels	level	NNS
1734	1735	.	.	.

1736	1740	Thus	thus	RB
1740	1741	,	,	,
1742	1745	the	the	DT
1746	1751	block	block	NN
1752	1754	in	in	IN
1755	1758	MHC	MHC	NNP
1759	1764	class	class	NN
1765	1767	II	ii	CD
1768	1780	inducibility	inducibility	NN
1781	1783	in	in	IN
1784	1796	RB-defective	rb-defective	JJ
1797	1802	cells	cell	NNS
1803	1805	is	be	VBZ
1806	1809	not	not	RB
1810	1813	due	due	JJ
1814	1816	to	to	TO
1817	1818	a	a	DT
1819	1824	block	block	NN
1825	1827	in	in	IN
1828	1833	CIITA	CIITA	NNP
1834	1846	inducibility	inducibility	NN
1846	1847	.	.	.