0	12	Microtubules	Microtubule	NNP
13	20	mediate	mediate	VBP
21	29	cellular	cellular	JJ
30	47	25-hydroxyvitamin	25-hydroxyvitamin	NN
48	50	D3	d3	NN
51	62	trafficking	trafficking	NN
63	66	and	and	CC
67	70	the	the	DT
71	78	genomic	genomic	JJ
79	87	response	response	NN
88	90	to	to	TO
91	112	1,25-dihydroxyvitamin	1,25-dihydroxyvitamin	NN
113	115	D3	d3	NN
116	118	in	in	IN
119	125	normal	normal	JJ
126	131	human	human	JJ
132	141	monocytes	monocyte	NNS
141	142	.	.	.

144	147	The	the	DT
148	155	genomic	genomic	JJ
156	163	actions	action	NNS
164	166	of	of	IN
167	188	1,25-dihydroxyvitamin	1,25-dihydroxyvitamin	NN
189	191	D3	d3	NN
192	193	(	(	(
193	205	1,25(OH)2D3)	1,25(oh)2d3)	NN
206	209	are	be	VBP
210	218	mediated	mediate	VBN
219	221	by	by	IN
222	225	the	the	DT
226	239	intracellular	intracellular	JJ
240	247	vitamin	vitamin	NN
248	249	D	D	NNP
250	258	receptor	receptor	NN
259	260	(	(	(
260	263	VDR	VDR	NNP
263	264	)	)	)
264	265	.	.	.

266	274	Although	although	IN
275	294	immunocytochemistry	immunocytochemistry	NN
295	298	has	have	VBZ
299	304	shown	show	VBN
305	309	that	that	IN
310	320	disruption	disruption	NN
321	323	of	of	IN
324	336	microtubular	microtubular	JJ
337	345	assembly	assembly	NN
346	354	prevents	prevent	VBZ
355	362	nuclear	nuclear	JJ
363	369	access	access	NN
370	372	of	of	IN
373	376	the	the	DT
377	387	sterol-VDR	sterol-VDR	NNP
388	395	complex	complex	NN
395	396	,	,	,
397	400	the	the	DT
401	405	role	role	NN
406	408	of	of	IN
409	421	microtubules	microtubule	NNS
422	424	in	in	IN
425	428	the	the	DT
429	437	response	response	NN
438	440	to	to	TO
441	452	1,25(OH)2D3	1,25(OH)2D3	NNP
453	456	has	have	VBZ
457	460	not	not	RB
461	465	been	be	VBN
466	473	studied	study	VBN
474	476	in	in	IN
477	483	viable	viable	JJ
484	489	cells	cell	NNS
489	490	.	.	.

491	494	Our	our	PRP$
495	502	studies	study	NNS
503	511	examined	examine	VBN
512	516	this	this	DT
517	528	interaction	interaction	NN
529	531	in	in	IN
532	538	normal	normal	JJ
539	544	human	human	JJ
545	554	monocytes	monocyte	NNS
554	555	.	.	.

556	565	Monocytes	monocyte	NNS
566	573	convert	convert	VBP
574	582	25(OH)D3	25(oh)d3	NN
583	585	to	to	TO
586	597	1,25(OH)2D3	1,25(oh)2d3	NN
598	601	and	and	CC
602	604	to	to	TO
605	620	24-hydroxylated	24-hydroxylate	VBN
621	632	metabolites	metabolite	NNS
633	637	more	more	RBR
638	643	polar	polar	JJ
644	648	than	than	IN
649	660	1,25(OH)2D3	1,25(OH)2D3	NNP
660	661	.	.	.

662	673	Microtubule	Microtubule	NNP
674	684	disruption	disruption	NN
685	692	totally	totally	RB
693	702	abolished	abolish	VBD
703	706	the	the	DT
707	714	ability	ability	NN
715	717	of	of	IN
718	727	exogenous	exogenous	JJ
728	739	1,25(OH)2D3	1,25(oh)2d3	NN
740	742	to	to	TO
743	751	suppress	suppress	VB
752	755	its	its	PRP$
756	759	own	own	JJ
760	769	synthesis	synthesis	NN
770	773	and	and	CC
774	776	to	to	TO
777	783	induce	induce	VB
784	798	24-hydroxylase	24-hydroxylase	NN
799	803	mRNA	mrna	NN
804	807	and	and	CC
808	816	activity	activity	NN
816	817	,	,	,
818	825	without	without	IN
826	835	affecting	affect	VBG
836	842	either	either	CC
843	848	total	total	JJ
849	860	1,25(OH)2D3	1,25(oh)2d3	NN
861	867	uptake	uptake	NN
868	870	or	or	CC
871	878	maximal	maximal	JJ
879	894	1,25(OH)2D3-VDR	1,25(oh)2d3-vdr	NN
895	902	binding	binding	NN
902	903	.	.	.

904	908	Thus	thus	RB
908	909	,	,	,
910	916	intact	intact	JJ
917	929	microtubules	microtubule	NNS
930	933	are	be	VBP
934	943	essential	essential	JJ
944	947	for	for	IN
948	969	1,25(OH)2D3-dependent	1,25(oh)2d3-dependent	JJ
970	980	modulation	modulation	NN
981	983	of	of	IN
984	988	gene	gene	NN
989	1002	transcription	transcription	NN
1002	1003	.	.	.

1004	1017	Interestingly	interestingly	RB
1017	1018	,	,	,
1019	1030	microtubule	microtubule	NN
1031	1041	disruption	disruption	NN
1042	1046	also	also	RB
1047	1056	decreased	decrease	VBD
1057	1065	monocyte	monocyte	NN
1066	1077	1,25(OH)2D3	1,25(oh)2d3	NN
1078	1087	synthesis	synthesis	NN
1087	1088	,	,	,
1089	1092	not	not	RB
1093	1095	by	by	IN
1096	1106	decreasing	decrease	VBG
1107	1110	the	the	DT
1111	1115	Vmax	Vmax	NNP
1116	1118	of	of	IN
1119	1127	monocyte	monocyte	NN
1128	1141	mitochondrial	mitochondrial	JJ
1142	1143	1	1	CD
1144	1161	alpha-hydroxylase	alpha-hydroxylase	NN
1162	1165	but	but	CC
1166	1173	through	through	IN
1174	1176	an	an	DT
1177	1185	increase	increase	NN
1186	1188	in	in	IN
1189	1192	the	the	DT
1193	1195	Km	Km	NNP
1196	1199	for	for	IN
1200	1209	25(OH)2D3	25(OH)2D3	NNP
1209	1210	.	.	.

1211	1213	We	we	PRP
1214	1222	examined	examine	VBD
1223	1231	25(OH)D3	25(oh)d3	NN
1232	1241	transport	transport	NN
1241	1242	.	.	.

1243	1254	Microtubule	Microtubule	NNP
1255	1265	disruption	disruption	NN
1266	1269	did	do	VBD
1270	1273	not	not	RB
1274	1280	affect	affect	VB
1281	1286	total	total	JJ
1287	1295	cellular	cellular	JJ
1296	1304	25(OH)D3	25(oh)d3	NN
1305	1311	uptake	uptake	NN
1312	1315	but	but	CC
1316	1323	reduced	reduce	VBD
1324	1327	its	its	PRP$
1328	1341	intracellular	intracellular	JJ
1342	1353	trafficking	trafficking	NN
1354	1356	to	to	TO
1357	1360	the	the	DT
1361	1373	mitochondria	mitochondrion	NNS
1373	1374	.	.	.

1375	1379	Thus	thus	RB
1379	1380	,	,	,
1381	1393	microtubules	microtubule	NNS
1394	1405	participate	participate	VBP
1406	1408	in	in	IN
1409	1422	intracellular	intracellular	JJ
1423	1431	25(OH)D3	25(oh)d3	NN
1432	1441	transport	transport	NN
1441	1442	,	,	,
1443	1446	and	and	CC
1447	1452	their	their	PRP$
1453	1462	integrity	integrity	NN
1463	1473	determines	determine	VBZ
1474	1480	normal	normal	JJ
1481	1492	1,25(OH)2D3	1,25(oh)2d3	NN
1493	1502	synthesis	synthesis	NN
1502	1503	.	.	.