0 14 Erythropoietin erythropoietin NN 15 25 stimulates stimulate VBZ 26 39 transcription transcription NN 40 42 of of IN 43 46 the the DT 47 55 TAL1/SCL TAL1/SCL NNP 56 60 gene gene NN 61 64 and and CC 65 80 phosphorylation phosphorylation NN 81 83 of of IN 84 87 its its PRP$ 88 95 protein protein NN 96 104 products product NNS 104 105 . . . 107 117 Activation Activation NNP 118 120 of of IN 121 124 the the DT 125 129 TAL1 tal1 NN 130 131 ( ( ( 131 133 or or CC 134 137 SCL SCL NNP 137 138 ) ) ) 139 143 gene gene NN 143 144 , , , 145 155 originally originally RB 156 166 identified identify VBN 167 174 through through IN 175 178 its its PRP$ 179 190 involvement involvement NN 191 193 by by IN 194 195 a a DT 196 205 recurrent recurrent JJ 206 217 chromosomal chromosomal JJ 218 231 translocation translocation NN 231 232 , , , 233 235 is be VBZ 236 239 the the DT 240 244 most most RBS 245 253 frequent frequent JJ 254 263 molecular molecular JJ 264 270 lesion lesion NN 271 281 recognized recognize VBN 282 284 in in IN 285 291 T-cell t-cell NN 292 297 acute acute JJ 298 311 lymphoblastic lymphoblastic JJ 312 320 leukemia leukemia NN 320 321 . . . 322 325 The the DT 326 333 protein protein NN 334 342 products product NNS 343 345 of of IN 346 350 this this DT 351 355 gene gene NN 356 363 contain contain VBP 364 367 the the DT 368 390 basic-helix-loop-helix basic-helix-loop-helix JJ 391 396 motif motif NN 397 411 characteristic characteristic JJ 412 414 of of IN 415 416 a a DT 417 422 large large JJ 423 429 family family NN 430 432 of of IN 433 446 transcription transcription NN 447 454 factors factor NNS 455 459 that that WDT 460 464 bind bind VBP 465 467 to to TO 468 471 the the DT 472 481 canonical canonical JJ 482 485 DNA dna NN 486 494 sequence sequence NN 495 501 CANNTG CANNTG NNP 502 504 as as IN 505 512 protein protein NN 513 525 heterodimers heterodimer NNS 525 526 . . . 527 531 TAL1 tal1 NN 532 542 expression expression NN 543 545 by by IN 546 555 erythroid erythroid JJ 556 561 cells cell NNS 562 564 in in FW 565 569 vivo vivo FW 570 573 and and CC 574 576 in in IN 577 593 chemical-induced chemical-induced JJ 594 609 erythroleukemia erythroleukemia NN 610 614 cell cell NN 615 620 lines line NNS 621 623 in in FW 624 628 vivo vivo FW 629 638 suggested suggest VBD 639 642 the the DT 643 647 gene gene NN 648 653 might might MD 654 662 regulate regulate VB 663 670 aspects aspect NNS 671 673 of of IN 674 683 erythroid erythroid JJ 684 699 differentiation differentiation NN 699 700 . . . 701 706 Since since IN 707 710 the the DT 711 719 terminal terminal JJ 720 726 events event NNS 727 729 of of IN 730 744 erythropoiesis erythropoiesis NN 745 748 are be VBP 749 759 controlled control VBN 760 762 by by IN 763 766 the the DT 767 779 glycoprotein glycoprotein NN 780 787 hormone hormone NN 788 802 erythropoietin erythropoietin NN 803 804 ( ( ( 804 807 Epo Epo NNP 807 808 ) ) ) 808 809 , , , 810 812 we we PRP 813 825 investigated investigate VBD 826 833 whether whether IN 834 837 the the DT 838 848 expression expression NN 849 851 or or CC 852 860 activity activity NN 861 863 of of IN 864 867 the the DT 868 872 TAL1 tal1 NN 873 877 gene gene NN 878 881 and and CC 882 885 its its PRP$ 886 893 protein protein NN 894 902 products product NNS 903 907 were be VBD 908 916 affected affect VBN 917 919 by by IN 920 923 Epo Epo NNP 924 926 in in IN 927 934 splenic splenic JJ 935 948 erythroblasts erythroblast NNS 949 953 from from IN 954 958 mice mouse NNS 959 967 infected infect VBN 968 972 with with IN 973 975 an an DT 976 991 anemia-inducing anemia-inducing JJ 992 998 strain strain NN 999 1001 of of IN 1002 1008 Friend Friend NNP 1009 1014 virus virus NN 1015 1016 ( ( ( 1016 1019 FVA FVA NNP 1020 1025 cells cell NNS 1025 1026 ) ) ) 1026 1027 . . . 1028 1031 Epo Epo NNP 1032 1040 elicited elicit VBD 1041 1042 a a DT 1043 1048 rapid rapid JJ 1048 1049 , , , 1050 1062 dose-related dose-related JJ 1063 1071 increase increase NN 1072 1074 in in IN 1075 1079 TAL1 TAL1 NNP 1080 1084 mRNA mRNA NNP 1085 1087 by by IN 1088 1098 increasing increase VBG 1099 1112 transcription transcription NN 1113 1115 of of IN 1116 1119 the the DT 1120 1124 gene gene NN 1125 1128 and and CC 1129 1140 stabilizing stabilize VBG 1141 1144 one one CD 1145 1147 of of IN 1148 1151 its its PRP$ 1152 1157 mRNAs mrna NNS 1157 1158 . . . 1159 1161 An an DT 1162 1175 Epo-inducible epo-inducible JJ 1176 1180 TAL1 TAL1 NNP 1181 1184 DNA DNA NNP 1185 1192 binding binding NN 1193 1201 activity activity NN 1202 1205 was be VBD 1206 1216 identified identify VBN 1217 1219 in in IN 1220 1223 FVA FVA NNP 1224 1228 cell cell NN 1229 1236 nuclear nuclear JJ 1237 1245 extracts extract NNS 1246 1250 that that WDT 1251 1263 subsequently subsequently RB 1264 1271 decayed decay VBD 1272 1279 despite despite IN 1280 1292 accumulating accumulate VBG 1293 1297 mRNA mrna NN 1298 1301 and and CC 1302 1309 protein protein NN 1309 1310 . . . 1311 1320 Induction Induction NNP 1321 1323 of of IN 1324 1327 DNA DNA NNP 1328 1335 binding binding NN 1336 1344 activity activity NN 1345 1348 was be VBD 1349 1359 associated associate VBN 1360 1370 temporally temporally RB 1371 1375 with with IN 1376 1387 Epo-induced epo-induced JJ 1388 1403 phosphorylation phosphorylation NN 1404 1406 of of IN 1407 1414 nuclear nuclear JJ 1415 1419 TAL1 tal1 NN 1420 1427 protein protein NN 1427 1428 . . . 1429 1434 These these DT 1435 1442 results result NNS 1443 1451 indicate indicate VBP 1452 1456 that that IN 1457 1460 Epo Epo NNP 1461 1465 acts act VBZ 1466 1468 at at IN 1469 1473 both both CC 1474 1489 transcriptional transcriptional JJ 1490 1493 and and CC 1494 1513 posttranscriptional posttranscriptional JJ 1514 1520 levels level NNS 1521 1523 on on IN 1524 1527 the the DT 1528 1532 TAL1 tal1 NN 1533 1538 locus locus NN 1539 1541 in in IN 1542 1548 Friend Friend NNP 1549 1562 virus-induced virus-induced JJ 1563 1576 erythroblasts erythroblast NNS 1577 1580 and and CC 1581 1590 establish establish VB 1591 1592 a a DT 1593 1597 link link NN 1598 1605 between between IN 1606 1609 Epo Epo NNP 1610 1619 signaling signaling NN 1620 1630 mechanisms mechanism NNS 1631 1634 and and CC 1635 1636 a a DT 1637 1643 member member NN 1644 1646 of of IN 1647 1648 a a DT 1649 1655 family family NN 1656 1658 of of IN 1659 1672 transcription transcription NN 1673 1680 factors factor NNS 1681 1689 involved involve VBN 1690 1692 in in IN 1693 1696 the the DT 1697 1712 differentiation differentiation NN 1713 1715 of of IN 1716 1723 diverse diverse JJ 1724 1728 cell cell NN 1729 1737 lineages lineage NNS 1737 1738 . . .