0 23 Glucocorticoid-mediated glucocorticoid-mediated JJ 24 34 inhibition inhibition NN 35 37 of of IN 38 51 interleukin-2 interleukin-2 NN 52 60 receptor receptor NN 61 66 alpha alpha NN 67 70 and and CC 71 76 -beta -beta NN 77 84 subunit subunit NN 85 95 expression expression NN 96 98 by by IN 99 104 human human JJ 105 106 T t NN 107 112 cells cell NNS 112 113 . . . 115 117 To to TO 118 127 determine determine VB 128 131 the the DT 132 141 mechanism mechanism NN 142 144 of of IN 145 159 glucocorticoid glucocorticoid NN 160 161 ( ( ( 161 173 GC)-mediated gc)-mediated JJ 174 184 inhibition inhibition NN 185 187 of of IN 188 189 T t NN 190 194 cell cell NN 195 204 functions function NNS 204 205 , , , 206 209 the the DT 210 216 effect effect NN 217 219 of of IN 220 233 dexamethasone dexamethasone NN 234 235 ( ( ( 235 237 DM DM NNP 237 238 ) ) ) 239 241 on on IN 242 243 T t NN 244 248 cell cell NN 249 262 proliferation proliferation NN 263 266 and and CC 267 280 interleukin-2 interleukin-2 NN 281 289 receptor receptor NN 290 291 ( ( ( 291 296 IL-2R IL-2R NNP 296 297 ) ) ) 298 308 generation generation NN 309 313 were be VBD 314 321 studied study VBN 321 322 . . . 323 336 Dexamethasone Dexamethasone NNP 337 346 inhibited inhibit VBD 347 359 IL-2-induced il-2-induced JJ 360 361 T t NN 362 366 cell cell NN 367 380 proliferation proliferation NN 381 383 by by IN 384 390 30%-88 30%-88 CD 390 391 % % NN 391 392 , , , 393 401 relative relative JJ 402 404 to to TO 405 408 its its PRP$ 409 422 concentration concentration NN 423 429 within within IN 430 433 the the DT 434 442 cultures culture NNS 442 443 . . . 444 447 The the DT 448 454 effect effect NN 455 457 of of IN 458 460 DM DM NNP 461 463 on on IN 464 474 expression expression NN 475 477 of of IN 478 483 IL-2R IL-2R NNP 484 489 alpha alpha NN 490 491 ( ( ( 491 494 Tac Tac NNP 494 495 , , , 496 499 p55 p55 NN 499 500 , , , 501 505 CD25 CD25 NNP 505 506 ) ) ) 507 510 and and CC 511 515 beta beta NN 516 517 ( ( ( 517 520 p75 p75 NN 520 521 ) ) ) 522 527 genes gene NNS 528 530 in in IN 531 540 activated activate VBN 541 542 T t NN 543 548 cells cell NNS 549 552 was be VBD 553 561 examined examine VBN 562 566 next next RB 566 567 . . . 568 570 In in IN 571 572 T t NN 573 578 cells cell NNS 579 589 stimulated stimulate VBN 590 594 with with IN 595 603 purified purify VBN 604 622 phytohemagglutinin phytohemagglutinin NN 623 624 ( ( ( 624 629 PHA-p PHA-p NNP 629 630 ) ) ) 631 634 and and CC 635 636 4 4 CD 637 649 beta-phorbol beta-phorbol NN 650 662 12-myristate 12-myristate NN 663 673 13-acetate 13-acetate NN 674 675 ( ( ( 675 678 PMA PMA NNP 678 679 ) ) ) 680 688 addition addition NN 689 691 of of IN 692 694 DM DM NNP 695 697 to to TO 698 701 the the DT 702 710 cultures culture NNS 711 719 resulted result VBD 720 722 in in IN 723 724 a a DT 725 727 60 60 CD 727 728 % % NN 729 738 reduction reduction NN 739 741 in in IN 742 747 IL-2R IL-2R NNP 748 753 alpha alpha NN 754 757 and and CC 758 759 a a DT 760 762 30 30 CD 762 763 % % NN 764 773 reduction reduction NN 774 776 in in IN 777 782 IL-2R IL-2R NNP 783 787 beta beta NN 788 796 membrane membrane NN 797 807 expression expression NN 808 816 compared compare VBN 817 819 to to TO 820 821 T t NN 822 827 cells cell NNS 828 836 cultured culture VBN 837 839 in in IN 840 843 the the DT 844 851 absence absence NN 852 854 of of IN 855 857 DM DM NNP 858 859 ( ( ( 859 860 p p NN 861 862 < < SYM 863 867 0.01 0.01 CD 867 868 ) ) ) 868 869 . . . 870 880 Inhibition Inhibition NNP 881 883 of of IN 884 892 membrane membrane NN 893 898 IL-2R il-2r NN 899 904 alpha alpha NN 905 908 and and CC 909 914 IL-2R il-2r NN 915 919 beta beta NN 920 930 expression expression NN 931 933 by by IN 934 940 10(-6) 10(-6) CD 941 942 M M NNP 943 945 DM DM NNP 946 949 was be VBD 950 959 partially partially RB 960 970 reversible reversible JJ 971 973 by by IN 974 985 recombinant recombinant JJ 986 991 human human JJ 992 996 IL-2 il-2 NN 997 998 ( ( ( 998 1004 rhIL-2 rhil-2 NN 1004 1005 ) ) ) 1005 1006 . . . 1007 1009 By by IN 1010 1018 Northern northern NN 1019 1023 blot blot NN 1024 1032 analysis analysis NN 1032 1033 , , , 1034 1036 DM DM NNP 1037 1043 caused cause VBD 1044 1045 a a DT 1046 1056 comparable comparable JJ 1057 1065 decrease decrease NN 1066 1068 in in IN 1069 1074 IL-2R IL-2R NNP 1075 1080 alpha alpha NN 1081 1084 and and CC 1085 1087 in in IN 1088 1093 IL-2R IL-2R NNP 1094 1098 beta beta NN 1099 1103 mRNA mrna NN 1104 1110 levels level NNS 1111 1113 to to TO 1114 1122 membrane membrane NN 1123 1131 receptor receptor NN 1132 1142 expression expression NN 1143 1145 in in IN 1146 1164 mitogen-stimulated mitogen-stimulated JJ 1165 1166 T t NN 1167 1172 cells cell NNS 1172 1173 . . . 1174 1176 By by IN 1177 1179 in in FW 1180 1185 vitro vitro FW 1186 1199 transcription transcription NN 1200 1206 assays assay NNS 1206 1207 , , , 1208 1210 DM DM NNP 1211 1220 regulated regulate VBD 1221 1226 IL-2R IL-2R NNP 1227 1232 alpha alpha NN 1233 1237 gene gene NN 1238 1248 expression expression NN 1249 1251 at at IN 1252 1253 a a DT 1254 1269 transcriptional transcriptional JJ 1270 1275 level level NN 1276 1281 while while IN 1282 1295 transcription transcription NN 1296 1298 of of IN 1299 1304 IL-2R IL-2R NNP 1305 1309 beta beta NN 1310 1314 gene gene NN 1315 1318 was be VBD 1319 1329 unaffected unaffected JJ 1330 1332 by by IN 1333 1335 DM DM NNP 1335 1336 . . . 1337 1340 The the DT 1341 1350 mechanism mechanism NN 1351 1353 of of IN 1354 1360 action action NN 1361 1363 of of IN 1364 1366 DM DM NNP 1367 1369 on on IN 1370 1375 IL-2R IL-2R NNP 1376 1381 alpha alpha NN 1382 1395 transcription transcription NN 1396 1399 was be VBD 1400 1408 examined examine VBN 1409 1411 by by IN 1412 1423 determining determine VBG 1424 1427 the the DT 1428 1432 mRNA mrna NN 1433 1439 levels level NNS 1440 1442 of of IN 1443 1446 the the DT 1447 1450 p50 p50 NN 1451 1458 subunit subunit NN 1459 1461 of of IN 1462 1469 nuclear nuclear JJ 1470 1476 factor factor NN 1477 1482 kappa kappa NN 1483 1484 B B NNP 1485 1486 ( ( ( 1486 1494 NF-kappa NF-kappa NNP 1495 1496 B B NNP 1496 1497 ) ) ) 1497 1498 , , , 1499 1500 a a DT 1501 1514 transcription transcription NN 1515 1521 factor factor NN 1522 1526 that that WDT 1527 1537 stimulates stimulate VBZ 1538 1543 IL-2R IL-2R NNP 1544 1549 alpha alpha NN 1550 1554 gene gene NN 1555 1565 expression expression NN 1565 1566 . . . 1567 1570 The the DT 1571 1575 data datum NNS 1576 1584 indicate indicate VBP 1585 1589 that that IN 1590 1596 10(-6) 10(-6) CD 1597 1598 M M NNP 1599 1601 DM DM NNP 1602 1611 increased increase VBD 1612 1613 T t NN 1614 1618 cell cell NN 1619 1622 p50 p50 NN 1623 1631 NF-kappa NF-kappa NNP 1632 1633 B B NNP 1634 1638 mRNA mRNA NNP 1639 1645 levels level NNS 1646 1648 by by IN 1649 1658 four-fold four-fold RB 1659 1667 compared compare VBN 1668 1670 to to TO 1671 1674 the the DT 1675 1681 levels level NNS 1682 1690 obtained obtain VBN 1691 1693 in in IN 1694 1697 the the DT 1698 1705 absence absence NN 1706 1708 of of IN 1709 1711 DM DM NNP 1711 1712 . . . 1713 1720 Further further RB 1720 1721 , , , 1722 1725 the the DT 1726 1731 level level NN 1732 1734 of of IN 1735 1742 nuclear nuclear JJ 1743 1751 proteins protein NNS 1752 1759 capable capable JJ 1760 1762 of of IN 1763 1770 binding bind VBG 1771 1773 to to TO 1774 1777 the the DT 1778 1786 NF-kappa NF-kappa NNP 1787 1788 B B NNP 1789 1794 sites site NNS 1795 1797 in in IN 1798 1807 activated activate VBN 1808 1809 T t NN 1810 1815 cells cell NNS 1816 1825 increased increase VBD 1826 1828 in in IN 1829 1837 response response NN 1838 1840 to to TO 1841 1843 DM DM NNP 1843 1844 . . . 1845 1847 In in IN 1848 1851 sum sum NN 1851 1852 , , , 1853 1855 DM DM NNP 1856 1865 regulates regulate VBZ 1866 1867 T t NN 1868 1872 cell cell NN 1873 1881 membrane membrane NN 1882 1892 expression expression NN 1893 1895 of of IN 1896 1901 IL-2R il-2r NN 1902 1904 by by IN 1905 1909 more more JJR 1910 1914 than than IN 1915 1918 one one CD 1919 1928 molecular molecular JJ 1929 1938 mechanism mechanism NN 1938 1939 . . .