0 13 Visualization Visualization NNP 14 16 of of IN 17 20 the the DT 21 31 endogenous endogenous JJ 32 40 NF-kappa NF-kappa NNP 41 42 B B NNP 43 46 p50 p50 NN 47 54 subunit subunit NN 55 57 in in IN 58 61 the the DT 62 69 nucleus nucleus NN 70 72 of of IN 73 83 follicular follicular JJ 84 93 dendritic dendritic JJ 94 99 cells cell NNS 100 102 in in IN 103 111 germinal germinal JJ 112 119 centers center NNS 119 120 . . . 122 130 NF-kappa NF-kappa NNP 131 132 B B NNP 132 133 , , , 134 135 a a DT 136 138 50 50 CD 139 145 kDa/65 kda/65 NN 146 149 kDa kda NN 150 151 ( ( ( 151 158 p50/p65 p50/p65 NN 158 159 ) ) ) 160 171 heterodimer heterodimer NN 171 172 , , , 173 175 is be VBZ 176 177 a a DT 178 188 ubiquitous ubiquitous JJ 189 202 transcription transcription NN 203 209 factor factor NN 210 218 involved involve VBN 219 221 in in IN 222 225 the the DT 226 234 positive positive JJ 235 245 regulation regulation NN 246 248 of of IN 249 256 various various JJ 257 263 immune immune JJ 264 269 genes gene NNS 269 270 . . . 271 274 The the DT 275 278 aim aim NN 279 281 of of IN 282 286 this this DT 287 292 study study NN 293 296 was be VBD 297 299 to to TO 300 309 determine determine VB 310 317 whether whether IN 318 326 NF-kappa NF-kappa NNP 327 328 B B NNP 329 331 is be VBZ 332 339 related related JJ 340 342 to to TO 343 344 a a DT 345 355 particular particular JJ 356 360 cell cell NN 361 365 type type NN 366 372 and/or and/or CC 373 388 differentiation differentiation NN 389 393 step step NN 394 400 during during IN 401 414 immunopoiesis immunopoiesis NN 414 415 . . . 416 421 Using use VBG 422 424 in in FW 425 429 situ situ FW 430 443 hybridization hybridization NN 444 446 on on IN 447 455 sections section NNS 456 460 from from IN 461 464 non non JJ 465 468 HIV HIV NNP 469 481 hyperplastic hyperplastic JJ 482 487 lymph lymph NN 488 493 nodes node NNS 493 494 , , , 495 497 we we PRP 498 503 found find VBD 504 508 that that IN 509 512 the the DT 513 517 gene gene NN 518 520 of of IN 521 524 the the DT 525 528 105 105 CD 529 532 kDa kda NN 533 542 precursor precursor NN 543 545 of of IN 546 549 p50 p50 NN 550 553 was be VBD 554 567 overexpressed overexpresse VBN 568 570 in in IN 571 574 the the DT 575 580 light light JJ 581 585 zone zone NN 586 588 of of IN 589 597 germinal germinal JJ 598 605 centers center NNS 605 606 , , , 607 611 with with IN 612 613 a a DT 614 621 network network NN 622 628 aspect aspect NN 628 629 , , , 630 635 which which WDT 636 645 suggested suggest VBD 646 649 the the DT 650 661 involvement involvement NN 662 664 of of IN 665 675 follicular follicular JJ 676 685 dendritic dendritic JJ 686 691 cells cell NNS 692 693 ( ( ( 693 696 FDC FDC NNP 696 697 ) ) ) 697 698 . . . 699 701 By by IN 702 722 immunohistochemistry immunohistochemistry NN 722 723 , , , 724 727 p50 p50 NN 728 735 protein protein NN 736 739 was be VBD 740 748 detected detect VBN 749 751 in in IN 752 755 the the DT 756 765 cytoplasm cytoplasm NN 766 769 and and CC 770 777 nucleus nucleus NN 778 780 of of IN 781 784 FDC FDC NNP 784 785 , , , 786 796 confirming confirm VBG 797 800 the the DT 801 812 involvement involvement NN 813 815 of of IN 816 819 FDC FDC NNP 819 820 . . . 821 832 Furthermore furthermore RB 832 833 , , , 834 837 p50 p50 NN 838 845 protein protein NN 846 849 was be VBD 850 858 detected detect VBN 859 861 in in IN 862 865 the the DT 866 875 cytoplasm cytoplasm NN 876 878 of of IN 879 882 all all DT 883 894 lymphocytes lymphocyte NNS 894 895 . . . 896 900 Thus thus RB 900 901 , , , 902 904 we we PRP 905 912 focused focus VBD 913 916 our our PRP$ 917 922 study study NN 923 925 on on IN 926 934 isolated isolate VBN 935 938 FDC FDC NNP 939 947 clusters cluster NNS 948 952 from from IN 953 959 normal normal JJ 960 967 tonsils tonsil NNS 967 968 . . . 969 971 As as IN 972 978 showed show VBN 979 981 on on IN 982 988 tissue tissue NN 989 997 sections section NNS 997 998 , , , 999 1001 we we PRP 1002 1010 detected detect VBD 1011 1014 the the DT 1015 1018 p50 p50 NN 1019 1021 in in IN 1022 1026 both both CC 1027 1036 cytoplasm cytoplasm NN 1037 1040 and and CC 1041 1048 nucleus nucleus NN 1049 1051 of of IN 1052 1055 FDC FDC NNP 1055 1056 . . . 1057 1063 Nuclei Nucleus NNP 1064 1066 of of IN 1067 1078 lymphocytes lymphocyte NNS 1079 1083 from from IN 1084 1087 FDC FDC NNP 1088 1096 clusters cluster NNS 1097 1101 were be VBD 1102 1110 negative negative JJ 1110 1111 . . . 1112 1114 We we PRP 1115 1119 next next RB 1120 1127 studied study VBD 1128 1131 p65 p65 NN 1132 1135 and and CC 1136 1141 c-Rel c-rel NN 1142 1149 protein protein NN 1150 1160 expression expression NN 1161 1163 in in IN 1164 1167 FDC FDC NNP 1168 1177 clusters. clusters. NNP 1178 1181 p65 p65 NN 1182 1185 was be VBD 1186 1194 detected detect VBN 1195 1197 in in IN 1198 1201 the the DT 1202 1211 cytoplasm cytoplasm NN 1212 1214 of of IN 1215 1218 FDC FDC NNP 1218 1219 , , , 1220 1227 whereas whereas IN 1228 1234 nuclei nucleus NNS 1235 1239 were be VBD 1240 1248 negative negative JJ 1248 1249 . . . 1250 1261 Furthermore furthermore RB 1261 1262 , , , 1263 1266 p65 p65 NN 1267 1270 was be VBD 1271 1279 detected detect VBN 1280 1282 in in IN 1283 1286 the the DT 1287 1293 nuclei nucleus NNS 1294 1296 of of IN 1297 1301 some some DT 1302 1314 lymphocytes. lymphocytes. JJ 1315 1320 c-Rel c-rel NN 1321 1328 protein protein NN 1329 1332 was be VBD 1333 1341 detected detect VBN 1342 1346 only only RB 1347 1349 in in IN 1350 1353 the the DT 1354 1363 cytoplasm cytoplasm NN 1364 1366 of of IN 1367 1378 lymphocytes lymphocyte NNS 1379 1382 and and CC 1383 1386 not not RB 1387 1389 in in IN 1390 1393 the the DT 1394 1401 nucleus nucleus NN 1402 1405 and and CC 1406 1415 cytoplasm cytoplasm NN 1416 1418 of of IN 1419 1422 FDC FDC NNP 1422 1423 . . . 1424 1427 Our our PRP$ 1428 1435 results result NNS 1436 1445 indicated indicate VBD 1446 1450 that that IN 1450 1451 , , , 1452 1454 in in IN 1455 1458 the the DT 1459 1466 context context NN 1467 1469 of of IN 1470 1471 T t NN 1472 1486 cell-dependent cell-dependent JJ 1487 1488 B B NNP 1489 1493 cell cell NN 1494 1507 immunopoiesis immunopoiesis NN 1508 1517 occurring occur VBG 1518 1520 in in IN 1521 1524 FDC FDC NNP 1525 1533 clusters cluster NNS 1533 1534 , , , 1535 1538 p50 p50 NN 1539 1541 is be VBZ 1542 1548 mainly mainly RB 1549 1556 related relate VBN 1557 1559 to to TO 1560 1563 FDC FDC NNP 1564 1568 with with IN 1569 1570 a a DT 1571 1578 massive massive JJ 1579 1593 overexpression overexpression NN 1594 1596 in in IN 1597 1600 the the DT 1601 1607 nuclei nucleus NNS 1607 1608 , , , 1609 1616 whereas whereas IN 1617 1620 p65 p65 NN 1621 1623 is be VBZ 1624 1633 expressed express VBN 1634 1636 in in IN 1637 1638 a a DT 1639 1648 scattered scattered JJ 1649 1655 manner manner NN 1656 1658 in in IN 1659 1662 the the DT 1663 1669 nuclei nucleus NNS 1670 1672 of of IN 1673 1684 lymphocytes lymphocyte NNS 1685 1688 and and CC 1689 1694 c-Rel c-rel NN 1695 1702 protein protein NN 1703 1714 exclusively exclusively RB 1715 1717 in in IN 1718 1721 the the DT 1722 1731 cytoplasm cytoplasm NN 1732 1734 of of IN 1735 1746 lymphocytes lymphocyte NNS 1747 1751 from from IN 1752 1755 FDC FDC NNP 1756 1764 clusters cluster NNS 1764 1765 . . . 1766 1771 These these DT 1772 1779 results result NNS 1780 1789 suggested suggest VBD 1790 1794 that that IN 1795 1798 the the DT 1799 1802 two two CD 1803 1811 subunits subunit NNS 1812 1814 of of IN 1815 1823 NF-kappa NF-kappa NNP 1824 1825 B B NNP 1826 1829 and and CC 1830 1833 the the DT 1834 1839 c-Rel c-rel NN 1840 1847 protein protein NN 1848 1852 have have VBP 1853 1862 different different JJ 1863 1868 roles role NNS 1869 1871 in in IN 1872 1881 different different JJ 1882 1886 cell cell NN 1887 1892 types type NNS 1893 1899 during during IN 1900 1901 B B NNP 1902 1906 cell cell NN 1907 1920 immunopoiesis immunopoiesis NN 1920 1921 . . .