0 16 Protein-tyrosine Protein-tyrosine NNP 17 23 kinase kinase NNP 24 34 activation activation NN 35 37 is be VBZ 38 46 required require VBN 47 50 for for IN 51 69 lipopolysaccharide lipopolysaccharide NN 70 79 induction induction NN 80 82 of of IN 83 94 interleukin interleukin NN 95 100 1beta 1beta NN 101 104 and and CC 105 113 NFkappaB nfkappab NN 114 124 activation activation NN 124 125 , , , 126 129 but but CC 130 133 not not RB 134 142 NFkappaB NFkappaB NNP 143 150 nuclear nuclear JJ 151 164 translocation translocation NN 164 165 . . . 167 169 In in IN 170 175 human human JJ 176 185 monocytes monocyte NNS 185 186 , , , 187 198 interleukin interleukin NN 199 204 1beta 1beta NN 205 212 protein protein NN 213 223 production production NN 224 227 and and CC 228 234 steady steady JJ 235 240 state state NN 241 245 mRNA mrna NN 246 252 levels level NNS 253 256 are be VBP 257 266 increased increase VBN 267 269 in in IN 270 278 response response NN 279 281 to to TO 282 300 lipopolysaccharide lipopolysaccharide NN 300 301 , , , 302 315 predominantly predominantly RB 316 318 as as IN 319 320 a a DT 321 327 result result NN 328 330 of of IN 331 340 increased increase VBN 341 354 transcription transcription NN 355 357 of of IN 358 361 the the DT 362 373 interleukin interleukin NN 374 379 1beta 1beta NN 380 384 gene gene NN 384 385 . . . 386 396 Expression expression NN 397 399 of of IN 400 411 interleukin interleukin NN 412 417 1beta 1beta NN 418 421 and and CC 422 427 other other JJ 428 437 cytokines cytokine NNS 437 438 , , , 439 443 such such JJ 444 446 as as IN 447 458 interleukin interleukin NN 459 460 6 6 CD 461 464 and and CC 465 470 tumor tumor NN 471 479 necrosis necrosis NN 480 486 factor factor NN 487 492 alpha alpha NN 492 493 , , , 494 497 has have VBZ 498 502 been be VBN 503 508 shown show VBN 509 511 to to TO 512 514 be be VB 515 524 dependent dependent JJ 525 527 on on IN 528 531 the the DT 532 542 activation activation NN 543 545 of of IN 546 549 the the DT 550 563 transcription transcription NN 564 570 factor factor NN 570 571 , , , 572 580 NFkappaB NFkappaB NNP 580 581 . . . 582 587 Since since IN 588 594 recent recent JJ 595 602 studies study NNS 603 607 have have VBP 608 613 shown show VBN 614 618 that that IN 619 645 lipopolysaccharide-induced lipopolysaccharide-induced JJ 646 654 tyrosine tyrosine NN 655 661 kinase kinase NNP 662 672 activation activation NN 673 675 is be VBZ 676 679 not not RB 680 688 required require VBN 689 692 for for IN 693 701 NFkappaB NFkappaB NNP 702 709 nuclear nuclear JJ 710 723 translocation translocation NN 723 724 , , , 725 727 we we PRP 728 734 sought seek VBD 735 737 to to TO 738 747 determine determine VB 748 755 whether whether IN 756 764 NFkappaB NFkappaB NNP 765 777 translocated translocate VBN 778 780 in in IN 781 784 the the DT 785 792 absence absence NN 793 795 of of IN 796 804 tyrosine tyrosine NN 805 811 kinase kinase NNP 812 820 activity activity NN 821 824 was be VBD 825 831 active active JJ 832 834 in in IN 835 846 stimulating stimulate VBG 847 860 transcription transcription NN 860 861 . . . 862 864 We we PRP 865 869 have have VBP 870 875 found find VBN 876 880 that that IN 880 881 , , , 882 884 in in IN 885 888 the the DT 889 894 human human JJ 895 908 pro-monocytic pro-monocytic JJ 909 913 cell cell NN 914 918 line line NN 918 919 , , , 920 925 THP-1 THP-1 NNP 925 926 , , , 927 930 the the DT 931 957 lipopolysaccharide-induced lipopolysaccharide-induced JJ 958 968 expression expression NN 969 971 of of IN 972 983 interleukin interleukin NN 984 989 1beta 1beta NN 990 992 is be VBZ 993 1002 dependent dependent JJ 1003 1005 on on IN 1006 1014 tyrosine tyrosine NN 1015 1021 kinase kinase NNP 1022 1032 activation activation NN 1032 1033 . . . 1034 1042 Tyrosine Tyrosine NNP 1043 1050 kinases kinase NNS 1051 1054 are be VBP 1055 1058 not not RB 1059 1067 required require VBN 1068 1071 for for IN 1072 1099 lipopolysaccharide-mediated lipopolysaccharide-mediated JJ 1100 1107 nuclear nuclear JJ 1108 1121 translocation translocation NN 1122 1124 of of IN 1125 1133 NFkappaB NFkappaB NNP 1133 1134 . . . 1135 1142 However however RB 1142 1143 , , , 1144 1146 in in IN 1147 1150 the the DT 1151 1158 absence absence NN 1159 1161 of of IN 1162 1170 tyrosine tyrosine NN 1171 1177 kinase kinase NNP 1178 1186 activity activity NN 1186 1187 , , , 1188 1191 the the DT 1192 1199 ability ability NN 1200 1202 of of IN 1203 1211 NFkappaB NFkappaB NNP 1212 1214 to to TO 1215 1224 stimulate stimulate VB 1225 1238 transcription transcription NN 1239 1241 is be VBZ 1242 1250 impaired impaired JJ 1250 1251 . . . 1252 1256 This this DT 1257 1267 inhibition inhibition NN 1268 1270 of of IN 1271 1284 transcription transcription NN 1285 1287 is be VBZ 1288 1296 specific specific JJ 1297 1300 for for IN 1301 1309 NFkappaB NFkappaB NNP 1309 1310 ; ; : 1311 1313 in in IN 1314 1317 the the DT 1318 1325 absence absence NN 1326 1328 of of IN 1329 1337 tyrosine tyrosine NN 1338 1344 kinase kinase NNP 1345 1353 activity activity NN 1353 1354 , , , 1355 1369 AP-1-dependent ap-1-dependent JJ 1370 1383 transcription transcription NN 1384 1386 is be VBZ 1387 1395 enhanced enhance VBN 1395 1396 . . . 1397 1402 These these DT 1403 1410 results result NNS 1411 1418 suggest suggest VBP 1419 1423 that that IN 1423 1424 , , , 1425 1430 while while IN 1431 1457 lipopolysaccharide-induced lipopolysaccharide-induced JJ 1458 1468 expression expression NN 1469 1471 of of IN 1472 1484 inflammatory inflammatory JJ 1485 1494 mediators mediator NNS 1495 1503 requires require VBZ 1504 1512 tyrosine tyrosine NN 1513 1519 kinase kinase NNP 1520 1528 activity activity NN 1528 1529 , , , 1530 1538 tyrosine tyrosine NN 1539 1545 kinase kinase NNP 1546 1554 activity activity NN 1555 1557 is be VBZ 1558 1561 not not RB 1562 1572 obligatory obligatory JJ 1573 1576 for for IN 1577 1595 lipopolysaccharide lipopolysaccharide NN 1596 1602 signal signal NN 1603 1615 transduction transduction NN 1615 1616 . . .