0 13 CD14-mediated cd14-mediated JJ 14 20 signal signal NN 21 28 pathway pathway NN 29 31 of of IN 32 45 Porphyromonas Porphyromonas NNP 46 56 gingivalis gingivalis NN 57 75 lipopolysaccharide lipopolysaccharide NN 76 78 in in IN 79 84 human human JJ 85 93 gingival gingival JJ 94 105 fibroblasts fibroblast NNS 105 106 . . . 108 126 Lipopolysaccharide Lipopolysaccharide NNP 127 128 ( ( ( 128 131 LPS LPS NNP 131 132 ) ) ) 133 140 induces induce VBZ 141 151 expression expression NN 152 154 of of IN 155 167 inflammatory inflammatory JJ 168 177 cytokines cytokine NNS 178 180 in in IN 181 202 monocytes/macrophages monocytes/macrophage NNS 203 206 via via IN 207 211 CD14 cd14 NN 211 212 , , , 213 216 one one CD 217 219 of of IN 220 223 the the DT 224 227 LPS LPS NNP 228 237 receptors receptor NNS 237 238 , , , 239 244 which which WDT 245 247 is be VBZ 248 257 expressed express VBN 258 271 predominantly predominantly RB 272 274 in in IN 275 280 these these DT 281 286 cells cell NNS 286 287 . . . 288 290 It it PRP 291 294 has have VBZ 295 299 been be VBN 300 312 demonstrated demonstrate VBN 313 317 that that IN 318 331 Porphyromonas Porphyromonas NNP 332 342 gingivalis gingivalis NN 343 346 LPS LPS NNP 347 348 ( ( ( 348 353 P-LPS P-LPS NNP 353 354 ) ) ) 355 359 also also RB 360 362 is be VBZ 363 367 able able JJ 368 370 to to TO 371 377 induce induce VB 378 390 inflammatory inflammatory JJ 391 400 cytokines cytokine NNS 401 403 in in IN 404 409 human human JJ 410 418 gingival gingival JJ 419 430 fibroblasts fibroblast NNS 430 431 . . . 432 441 Therefore therefore RB 441 442 , , , 443 445 it it PRP 446 448 is be VBZ 449 458 important important JJ 459 461 to to TO 462 471 determine determine VB 472 479 whether whether IN 480 484 CD14 cd14 NN 485 487 is be VBZ 488 497 expressed express VBN 498 500 in in IN 501 509 gingival gingival JJ 510 521 fibroblasts fibroblast NNS 522 525 and and CC 526 528 to to TO 529 535 define define VB 536 539 the the DT 540 554 P-LPS-mediated p-lps-mediated JJ 555 573 signal-transducing signal-transducing JJ 574 583 mechanism mechanism NN 584 586 in in IN 587 590 the the DT 591 596 cells cell NNS 596 597 . . . 598 600 In in IN 601 605 this this DT 606 611 study study NN 611 612 , , , 613 615 we we PRP 616 624 observed observe VBD 625 637 unexpectedly unexpectedly RB 638 640 by by IN 641 660 immunohistochemical immunohistochemical JJ 660 661 , , , 662 669 Western western JJ 670 678 blotting blotting NN 679 680 ( ( ( 680 694 immunoblotting immunoblotting NN 694 695 ) ) ) 695 696 , , , 697 700 and and CC 701 709 Northern Northern NNP 710 711 ( ( ( 711 714 RNA RNA NNP 714 715 ) ) ) 716 724 blotting blotting NN 725 731 assays assay NNS 732 736 that that IN 737 741 CD14 cd14 NN 742 744 is be VBZ 745 754 expressed express VBN 755 757 at at IN 758 762 high high JJ 763 770 density density NN 771 773 in in IN 774 779 human human JJ 780 788 gingival gingival JJ 789 800 fibroblasts fibroblast NNS 800 801 . . . 802 815 P-LPS-induced p-lps-induced JJ 816 826 expression expression NN 827 829 of of IN 830 833 the the DT 834 842 monocyte monocyte NN 843 858 chemoattractant chemoattractant NN 859 866 protein protein NN 867 868 1 1 CD 869 870 ( ( ( 870 875 MCP-1 mcp-1 NN 875 876 ) ) ) 877 881 gene gene NN 882 884 in in IN 885 888 the the DT 889 894 cells cell NNS 895 898 was be VBD 899 908 inhibited inhibit VBN 909 917 markedly markedly RB 918 920 by by IN 921 930 treatment treatment NN 931 935 with with IN 936 946 anti-human anti-human JJ 947 951 CD14 cd14 NN 952 960 antibody antibody NN 961 964 and and CC 965 968 was be VBD 969 979 completely completely RB 980 989 inhibited inhibit VBN 990 992 by by IN 993 1003 herbimycin herbimycin NN 1004 1005 A A NNP 1005 1006 , , , 1007 1008 a a DT 1009 1015 potent potent JJ 1016 1025 inhibitor inhibitor NN 1026 1028 of of IN 1029 1037 tyrosine tyrosine NN 1038 1044 kinase kinase NNP 1044 1045 . . . 1046 1049 The the DT 1050 1059 inhibitor inhibitor NN 1060 1064 also also RB 1065 1077 dramatically dramatically RB 1078 1087 inhibited inhibit VBD 1088 1096 monocyte monocyte NN 1097 1108 chemotactic chemotactic JJ 1109 1117 activity activity NN 1118 1120 of of IN 1121 1124 and and CC 1125 1130 MCP-1 mcp-1 NN 1131 1141 production production NN 1142 1144 by by IN 1145 1148 the the DT 1149 1154 cells cell NNS 1154 1155 . . . 1156 1167 Furthermore furthermore RB 1167 1168 , , , 1169 1182 P-LPS-induced p-lps-induced JJ 1183 1193 expression expression NN 1194 1196 of of IN 1197 1200 the the DT 1201 1206 MCP-1 mcp-1 NN 1207 1211 gene gene NN 1212 1214 in in IN 1215 1218 the the DT 1219 1224 cells cell NNS 1225 1229 also also RB 1230 1233 was be VBD 1234 1241 blocked block VBN 1242 1244 by by IN 1245 1255 inhibitors inhibitor NNS 1256 1258 of of IN 1259 1262 two two CD 1263 1276 transcription transcription NN 1277 1284 factors factor NNS 1284 1285 , , , 1286 1290 i.e. i.e. FW 1290 1291 , , , 1292 1300 curcumin curcumin NN 1300 1301 , , , 1302 1304 an an DT 1305 1314 inhibitor inhibitor NN 1315 1317 of of IN 1318 1322 AP-1 ap-1 NN 1322 1323 , , , 1324 1327 and and CC 1328 1338 pyrolidine pyrolidine NN 1339 1354 dithiocarbamate dithiocarbamate NN 1354 1355 , , , 1356 1358 an an DT 1359 1368 inhibitor inhibitor NN 1369 1371 of of IN 1372 1381 NF-kappaB NF-kappaB NNP 1381 1382 . . . 1383 1387 Both both DT 1388 1398 inhibitors inhibitor NNS 1399 1408 inhibited inhibit VBD 1409 1417 monocyte monocyte NN 1418 1429 chemotactic chemotactic JJ 1430 1438 activity activity NN 1439 1441 in in IN 1442 1445 the the DT 1446 1453 culture culture NN 1454 1465 supernatant supernatant NN 1466 1468 of of IN 1469 1482 P-LPS-treated p-lps-treated JJ 1483 1488 cells cell NNS 1488 1489 . . . 1490 1493 Gel Gel NNP 1494 1499 shift shift NN 1500 1508 mobility mobility NN 1509 1514 assay assay NN 1515 1521 showed show VBD 1522 1533 stimulation stimulation NN 1534 1536 of of IN 1537 1540 the the DT 1541 1545 AP-1 ap-1 NN 1546 1549 and and CC 1550 1559 NF-kappaB nf-kappab NN 1560 1568 contents content NNS 1569 1571 in in IN 1572 1585 P-LPS-treated p-lps-treated JJ 1586 1591 cells cell NNS 1591 1592 . . . 1593 1597 This this DT 1598 1603 study study NN 1604 1606 is be VBZ 1607 1610 the the DT 1611 1616 first first JJ 1617 1619 to to TO 1620 1631 demonstrate demonstrate VB 1632 1635 the the DT 1636 1646 expression expression NN 1647 1649 of of IN 1650 1654 CD14 cd14 NN 1655 1657 in in IN 1658 1663 human human JJ 1664 1672 gingival gingival JJ 1673 1684 fibroblasts fibroblast NNS 1685 1688 and and CC 1689 1691 to to TO 1692 1696 show show VB 1697 1701 that that IN 1702 1705 the the DT 1706 1724 signal-transducing signal-transducing JJ 1725 1732 pathway pathway NN 1733 1735 of of IN 1736 1741 P-LPS p-lps NN 1742 1744 in in IN 1745 1748 the the DT 1749 1754 cells cell NNS 1755 1757 is be VBZ 1758 1766 mediated mediate VBN 1767 1769 by by IN 1770 1774 CD14 cd14 NN 1774 1775 . . .