0 13 Interleukin-6 Interleukin-6 NNP 14 24 production production NN 25 27 in in IN 28 39 hemorrhagic hemorrhagic JJ 40 45 shock shock NN 46 48 is be VBZ 49 60 accompanied accompany VBN 61 63 by by IN 64 74 neutrophil neutrophil NN 75 86 recruitment recruitment NN 87 90 and and CC 91 95 lung lung NN 96 102 injury injury NN 102 103 . . . 105 116 Hemorrhagic hemorrhagic JJ 117 122 shock shock NN 123 124 ( ( ( 124 126 HS HS NNP 126 127 ) ) ) 128 137 initiates initiate VBZ 138 140 an an DT 141 153 inflammatory inflammatory JJ 154 161 cascade cascade NN 162 166 that that WDT 167 175 includes include VBZ 176 179 the the DT 180 190 production production NN 191 193 of of IN 194 203 cytokines cytokine NNS 204 207 and and CC 208 219 recruitment recruitment NN 220 222 of of IN 223 234 neutrophils neutrophil NNS 235 236 ( ( ( 236 239 PMN PMN NNP 239 240 ) ) ) 241 244 and and CC 245 248 may may MD 249 257 progress progress VB 258 260 to to TO 261 266 organ organ NN 267 274 failure failure NN 274 275 , , , 276 284 inducing induce VBG 285 290 acute acute JJ 291 302 respiratory respiratory JJ 303 311 distress distress NN 312 320 syndrome syndrome NN 321 322 ( ( ( 322 326 ARDS ards NN 326 327 ) ) ) 327 328 . . . 329 331 To to TO 332 339 examine examine VB 340 343 the the DT 344 354 hypothesis hypothesis NN 355 359 that that IN 360 373 interleukin-6 interleukin-6 NN 374 375 ( ( ( 375 379 IL-6 il-6 NN 379 380 ) ) ) 381 392 contributes contribute VBZ 393 395 to to TO 396 399 PMN pmn NN 400 412 infiltration infiltration NN 413 416 and and CC 417 421 lung lung NN 422 428 damage damage NN 429 431 in in IN 432 434 HS HS NNP 434 435 , , , 436 438 we we PRP 439 447 examined examine VBD 448 451 the the DT 452 457 lungs lung NNS 458 460 of of IN 461 465 rats rat NNS 466 475 subjected subject VBN 476 478 to to TO 479 493 unresuscitated unresuscitated JJ 494 497 and and CC 498 510 resuscitated resuscitated JJ 511 513 HS hs NN 514 517 for for IN 518 521 the the DT 522 532 production production NN 533 535 of of IN 536 540 IL-6 il-6 NN 541 544 and and CC 545 555 activation activation NN 556 558 of of IN 559 564 Stat3 Stat3 NNP 564 565 . . . 566 571 Using use VBG 572 588 semiquantitative semiquantitative JJ 589 595 RT-PCR rt-pcr NN 595 596 , , , 597 599 we we PRP 600 605 found find VBD 606 607 a a DT 608 616 striking striking JJ 617 625 increase increase NN 626 628 in in IN 629 633 IL-6 IL-6 NNP 634 638 mRNA mRNA NNP 639 645 levels level NNS 646 650 only only RB 651 653 in in IN 654 666 resuscitated resuscitated JJ 667 669 HS hs NN 669 670 , , , 671 675 with with IN 676 680 peak peak NN 681 687 levels level NNS 688 696 observed observe VBD 697 698 1 1 CD 699 700 h h NN 701 706 after after IN 707 717 initiation initiation NN 718 720 of of IN 721 734 resuscitation resuscitation NN 734 735 . . . 736 745 Increased increase VBN 746 750 IL-6 il-6 NN 751 758 protein protein NN 759 769 expression expression NN 770 773 was be VBD 774 783 localized localized JJ 784 786 to to TO 787 796 bronchial bronchial JJ 797 800 and and CC 801 809 alveolar alveolar JJ 810 815 cells cell NNS 815 816 . . . 817 832 Electrophoretic electrophoretic JJ 833 841 mobility mobility NN 842 847 shift shift NN 848 853 assay assay NN 854 856 of of IN 857 864 protein protein NN 865 873 extracts extract NNS 874 878 from from IN 879 884 shock shock NN 885 890 lungs lung NNS 891 900 exhibited exhibit VBD 901 903 an an DT 904 912 increase increase NN 913 915 in in IN 916 921 Stat3 stat3 NN 922 932 activation activation NN 933 937 with with IN 938 946 kinetics kinetic NNS 947 954 similar similar JJ 955 957 to to TO 958 962 IL-6 IL-6 NNP 963 967 mRNA mRNA NNP 967 968 . . . 969 971 In in FW 972 976 situ situ FW 977 980 DNA dna NN 981 988 binding binding NN 989 994 assay assay NN 995 1005 determined determine VBD 1006 1011 Stat3 stat3 NN 1012 1022 activation activation NN 1023 1036 predominantly predominantly RB 1037 1043 within within IN 1044 1051 alveoli alveolus NNS 1051 1052 . . . 1053 1066 Intratracheal intratracheal JJ 1067 1079 instillation instillation NN 1080 1082 of of IN 1083 1087 IL-6 il-6 NN 1088 1093 alone alone RB 1094 1098 into into IN 1099 1105 normal normal JJ 1106 1110 rats rat NNS 1111 1119 resulted result VBD 1120 1122 in in IN 1123 1126 PMN pmn NN 1127 1139 infiltration infiltration NN 1140 1144 into into IN 1145 1149 lung lung NN 1150 1162 interstitium interstitium NN 1163 1166 and and CC 1167 1174 alveoli alveolus NNS 1174 1175 , , , 1176 1182 marked marked JJ 1183 1192 elevation elevation NN 1193 1195 of of IN 1196 1211 bronchoalveolar bronchoalveolar JJ 1212 1218 lavage lavage NN 1219 1230 cellularity cellularity NN 1230 1231 , , , 1232 1235 and and CC 1236 1245 increased increase VBN 1246 1256 wet-to-dry wet-to-dry JJ 1257 1262 ratio ratio NN 1262 1263 . . . 1264 1269 These these DT 1270 1278 findings finding NNS 1279 1287 indicate indicate VBP 1288 1292 that that IN 1293 1297 IL-6 il-6 NN 1298 1308 production production NN 1309 1312 and and CC 1313 1318 Stat3 stat3 NN 1319 1329 activation activation NN 1330 1335 occur occur VBP 1336 1341 early early RB 1342 1344 in in IN 1345 1347 HS HS NNP 1348 1351 and and CC 1352 1355 may may MD 1356 1366 contribute contribute VB 1367 1369 to to TO 1370 1382 PMN-mediated pmn-mediated JJ 1383 1387 lung lung NN 1388 1394 injury injury NN 1394 1395 , , , 1396 1405 including include VBG 1406 1410 ARDS ARDS NNPS 1411 1416 after after IN 1417 1419 HS HS NNP 1419 1420 . . .