0 8 Reactive reactive JJ 9 15 oxygen oxygen NN 16 38 intermediate-dependent intermediate-dependent JJ 39 48 NF-kappaB NF-kappaB NNP 49 59 activation activation NN 60 62 by by IN 63 80 interleukin-1beta interleukin-1beta NN 81 89 requires require VBZ 90 104 5-lipoxygenase 5-lipoxygenase NN 105 107 or or CC 108 113 NADPH NADPH NNP 114 121 oxidase oxidase NN 122 130 activity activity NN 130 131 . . . 133 135 We we PRP 136 146 previously previously RB 147 155 reported report VBD 156 160 that that IN 161 164 the the DT 165 169 role role NN 170 172 of of IN 173 181 reactive reactive JJ 182 188 oxygen oxygen NN 189 202 intermediates intermediate NNS 203 204 ( ( ( 204 208 ROIs ROI NNP 208 209 ) ) ) 210 212 in in IN 213 222 NF-kappaB NF-kappaB NNP 223 233 activation activation NN 234 236 by by IN 237 252 proinflammatory proinflammatory JJ 253 262 cytokines cytokine NNS 263 266 was be VBD 267 271 cell cell NN 272 280 specific specific JJ 280 281 . . . 282 289 However however RB 289 290 , , , 291 294 the the DT 295 302 sources source NNS 303 306 for for IN 307 311 ROIs roi NNS 312 314 in in IN 315 322 various various JJ 323 327 cell cell NN 328 333 types type NNS 334 337 are be VBP 338 341 yet yet RB 342 344 to to TO 345 347 be be VB 348 358 determined determine VBN 359 362 and and CC 363 368 might might MD 369 376 include include VB 377 391 5-lipoxygenase 5-lipoxygenase NN 392 393 ( ( ( 393 398 5-LOX 5-lox NN 398 399 ) ) ) 400 403 and and CC 404 409 NADPH NADPH NNP 410 417 oxidase oxidase NN 417 418 . . . 419 424 5-LOX 5-lox NN 425 428 and and CC 429 434 5-LOX 5-lox NN 435 445 activating activating NN 446 453 protein protein NN 454 455 ( ( ( 455 459 FLAP FLAP NNP 459 460 ) ) ) 461 464 are be VBP 465 476 coexpressed coexpresse VBN 477 479 in in IN 480 488 lymphoid lymphoid JJ 489 494 cells cell NNS 495 498 but but CC 499 502 not not RB 503 505 in in IN 506 515 monocytic monocytic JJ 516 518 or or CC 519 529 epithelial epithelial JJ 530 535 cells cell NNS 535 536 . . . 537 548 Stimulation stimulation NN 549 551 of of IN 552 560 lymphoid lymphoid JJ 561 566 cells cell NNS 567 571 with with IN 572 589 interleukin-1beta interleukin-1beta NN 590 591 ( ( ( 591 599 IL-1beta IL-1beta NNP 599 600 ) ) ) 601 604 led lead VBD 605 607 to to TO 608 611 ROI ROI NNP 612 622 production production NN 623 626 and and CC 627 636 NF-kappaB nf-kappab NN 637 647 activation activation NN 647 648 , , , 649 654 which which WDT 655 660 could could MD 661 665 both both DT 666 668 be be VB 669 676 blocked block VBN 677 679 by by IN 680 692 antioxidants antioxidant NNS 693 695 or or CC 696 700 FLAP FLAP NNP 701 711 inhibitors inhibitor NNS 711 712 , , , 713 723 confirming confirm VBG 724 728 that that IN 729 734 5-LOX 5-lox NN 735 738 was be VBD 739 742 the the DT 743 749 source source NN 750 752 of of IN 753 757 ROIs ROI NNP 758 761 and and CC 762 765 was be VBD 766 774 required require VBN 775 778 for for IN 779 788 NF-kappaB NF-kappaB NNP 789 799 activation activation NN 800 802 in in IN 803 808 these these DT 809 814 cells cell NNS 814 815 . . . 816 824 IL-1beta IL-1beta NNP 825 836 stimulation stimulation NN 837 839 of of IN 840 850 epithelial epithelial JJ 851 856 cells cell NNS 857 860 did do VBD 861 864 not not RB 865 873 generate generate VB 874 877 any any DT 878 882 ROIs ROI NNPS 883 886 and and CC 887 896 NF-kappaB NF-kappaB NNP 897 906 induction induction NN 907 910 was be VBD 911 914 not not RB 915 925 influenced influence VBN 926 928 by by IN 929 934 5-LOX 5-lox NN 935 945 inhibitors inhibitor NNS 945 946 . . . 947 954 However however RB 954 955 , , , 956 970 reintroduction reintroduction NN 971 973 of of IN 974 975 a a DT 976 986 functional functional JJ 987 992 5-LOX 5-lox NN 993 999 system system NN 1000 1002 in in IN 1003 1008 these these DT 1009 1014 cells cell NNS 1015 1022 allowed allow VBD 1023 1026 ROI ROI NNP 1027 1037 production production NN 1038 1041 and and CC 1042 1057 5-LOX-dependent 5-lox-dependent JJ 1058 1067 NF-kappaB NF-kappaB NNP 1068 1078 activation activation NN 1078 1079 . . . 1080 1082 In in IN 1083 1092 monocytic monocytic JJ 1093 1098 cells cell NNS 1098 1099 , , , 1100 1108 IL-1beta IL-1beta NNP 1109 1118 treatment treatment NN 1119 1122 led lead VBD 1123 1125 to to TO 1126 1127 a a DT 1128 1138 production production NN 1139 1141 of of IN 1142 1146 ROIs ROI NNP 1147 1152 which which WDT 1153 1155 is be VBZ 1156 1167 independent independent JJ 1168 1170 of of IN 1171 1174 the the DT 1175 1180 5-LOX 5-lox NN 1181 1187 enzyme enzyme NN 1188 1191 but but CC 1192 1200 requires require VBZ 1201 1204 the the DT 1205 1210 NADPH NADPH NNP 1211 1218 oxidase oxidase NN 1219 1227 activity activity NN 1227 1228 . . . 1229 1233 This this DT 1234 1241 pathway pathway NN 1242 1250 involves involve VBZ 1251 1254 the the DT 1255 1259 Rac1 rac1 NN 1260 1263 and and CC 1264 1269 Cdc42 cdc42 NN 1270 1277 GTPases gtpas NNS 1277 1278 , , , 1279 1282 two two CD 1283 1290 enzymes enzyme NNS 1291 1296 which which WDT 1297 1300 are be VBP 1301 1304 not not RB 1305 1313 required require VBN 1314 1317 for for IN 1318 1327 NF-kappaB NF-kappaB NNP 1328 1338 activation activation NN 1339 1341 by by IN 1342 1350 IL-1beta IL-1beta NNP 1351 1353 in in IN 1354 1364 epithelial epithelial JJ 1365 1370 cells cell NNS 1370 1371 . . . 1372 1374 In in IN 1375 1385 conclusion conclusion NN 1385 1386 , , , 1387 1392 three three CD 1393 1402 different different JJ 1403 1416 cell-specific cell-specific JJ 1417 1425 pathways pathway NNS 1426 1430 lead lead VBP 1431 1433 to to TO 1434 1443 NF-kappaB NF-kappaB NNP 1444 1454 activation activation NN 1455 1457 by by IN 1458 1466 IL-1beta IL-1beta NNP 1466 1467 : : : 1468 1469 a a DT 1470 1477 pathway pathway NN 1478 1487 dependent dependent JJ 1488 1490 on on IN 1491 1494 ROI ROI NNP 1495 1505 production production NN 1506 1508 by by IN 1509 1514 5-LOX 5-lox NN 1515 1517 in in IN 1518 1526 lymphoid lymphoid JJ 1527 1532 cells cell NNS 1532 1533 , , , 1534 1536 an an DT 1537 1541 ROI- ROI- NNP 1542 1545 and and CC 1546 1563 5-LOX-independent 5-lox-independent JJ 1564 1571 pathway pathway NN 1572 1574 in in IN 1575 1585 epithelial epithelial JJ 1586 1591 cells cell NNS 1591 1592 , , , 1593 1596 and and CC 1597 1598 a a DT 1599 1606 pathway pathway NN 1607 1616 requiring require VBG 1617 1620 ROI ROI NNP 1621 1631 production production NN 1632 1634 by by IN 1635 1640 NADPH NADPH NNP 1641 1648 oxidase oxidase NN 1649 1651 in in IN 1652 1661 monocytic monocytic JJ 1662 1667 cells cell NNS 1667 1668 . . .