0 7 Nuclear nuclear JJ 8 20 factor-kappa factor-kappa NN 21 22 B B NNP 23 31 activity activity NN 32 34 in in IN 35 36 T t NN 37 42 cells cell NNS 43 47 from from IN 48 56 patients patient NNS 57 61 with with IN 62 71 rheumatic rheumatic JJ 72 80 diseases disease NNS 80 81 : : : 82 83 a a DT 84 95 preliminary preliminary JJ 96 102 report report NN 102 103 . . . 105 114 OBJECTIVE objective NN 114 115 : : : 116 119 The the DT 120 128 NF-kappa NF-kappa NNP 129 134 B/Rel B/Rel NNP 135 141 family family NN 142 144 of of IN 145 158 transcription transcription NN 159 166 factors factor NNS 167 176 regulates regulate VBZ 177 180 the the DT 181 191 expression expression NN 192 194 of of IN 195 199 many many JJ 200 205 genes gene NNS 206 214 involved involve VBN 215 217 in in IN 218 221 the the DT 222 228 immune immune JJ 229 231 or or CC 232 244 inflammatory inflammatory JJ 245 253 response response NN 254 256 at at IN 257 260 the the DT 261 276 transcriptional transcriptional JJ 277 282 level level NN 282 283 . . . 284 287 The the DT 288 291 aim aim NN 292 294 of of IN 295 299 this this DT 300 305 study study NN 306 309 was be VBD 310 312 to to TO 313 322 determine determine VB 323 330 whether whether IN 331 342 distinctive distinctive JJ 343 351 patterns pattern NNS 352 354 of of IN 355 363 NF-kappa NF-kappa NNP 364 365 B B NNP 366 376 activation activation NN 377 380 are be VBP 381 385 seen see VBN 386 388 in in IN 389 398 different different JJ 399 404 forms form NNS 405 407 of of IN 408 413 joint joint JJ 414 421 disease disease NN 421 422 . . . 423 430 METHODS methods NNS 430 431 : : : 432 435 The the DT 436 439 DNA dna NN 440 447 binding binding NN 448 456 activity activity NN 457 459 of of IN 460 465 these these DT 466 480 nucleoproteins nucleoprotein NNS 481 484 was be VBD 485 493 examined examine VBN 494 496 in in IN 497 505 purified purify VBN 506 514 synovial synovial JJ 515 518 and and CC 519 529 peripheral peripheral JJ 530 531 T t NN 532 537 cells cell NNS 538 542 from from IN 543 551 patients patient NNS 552 556 with with IN 557 564 various various JJ 565 572 chronic chronic JJ 573 582 rheumatic rheumatic JJ 583 591 diseases disease NNS 592 593 ( ( ( 593 595 12 12 CD 595 596 : : : 597 601 four four CD 602 606 with with IN 607 617 rheumatoid rheumatoid JJ 618 627 arthritis arthritis NN 627 628 ; ; : 629 633 five five CD 634 638 with with IN 639 660 spondyloarthropathies spondyloarthropathy NNS 660 661 ; ; : 662 665 and and CC 666 671 three three CD 672 676 with with IN 677 691 osteoarthritis osteoarthritis NN 691 692 ) ) ) 692 693 . . . 694 701 RESULTS results NNS 701 702 : : : 703 718 Electrophoretic electrophoretic JJ 719 727 mobility mobility NN 728 733 shift shift NN 734 740 assays assay NNS 741 750 disclosed disclose VBD 751 754 two two CD 755 763 specific specific JJ 764 773 complexes complex NNS 774 779 bound bind VBN 780 782 to to TO 783 784 a a DT 785 793 NF-kappa NF-kappa NNP 794 795 B B NNP 796 804 specific specific JJ 805 817 32P-labelled 32p-labelled JJ 818 833 oligonucleotide oligonucleotide NN 834 836 in in IN 837 851 nucleoproteins nucleoprotein NNS 852 861 extracted extract VBN 862 866 from from IN 867 875 purified purify VBN 876 877 T t NN 878 883 cells cell NNS 884 892 isolated isolate VBN 893 897 from from IN 898 906 synovial synovial JJ 907 912 fluid fluid NN 913 916 and and CC 917 927 peripheral peripheral JJ 928 933 blood blood NN 934 936 of of IN 937 945 patients patient NNS 946 950 with with IN 951 961 rheumatoid rheumatoid JJ 962 971 arthritis arthritis NN 971 972 . . . 973 976 The the DT 977 986 complexes complex NNS 987 996 consisted consist VBD 997 999 of of IN 1000 1007 p50/p50 p50/p50 NN 1008 1018 homodimers homodimer NNS 1019 1022 and and CC 1023 1030 p50/p65 p50/p65 NN 1031 1043 heterodimers heterodimer NNS 1043 1044 . . . 1045 1054 Increased increase VBN 1055 1063 NF-kappa NF-kappa NNP 1064 1065 B B NNP 1066 1073 binding bind VBG 1074 1076 to to TO 1077 1080 DNA DNA NNP 1081 1083 in in IN 1084 1092 synovial synovial JJ 1093 1094 T t NN 1095 1100 cells cell NNS 1101 1104 was be VBD 1105 1113 observed observe VBN 1114 1122 relative relative JJ 1123 1125 to to TO 1126 1136 peripheral peripheral JJ 1137 1138 T t NN 1139 1144 cells cell NNS 1144 1145 . . . 1146 1148 In in IN 1149 1163 non-rheumatoid non-rheumatoid JJ 1164 1173 arthritis arthritis NN 1173 1174 , , , 1175 1182 binding binding NN 1183 1185 of of IN 1186 1194 NF-kappa NF-kappa NNP 1195 1196 B B NNP 1197 1199 in in IN 1200 1208 synovial synovial JJ 1209 1210 T t NN 1211 1216 cells cell NNS 1217 1220 was be VBD 1221 1232 exclusively exclusively RB 1233 1241 mediated mediate VBN 1242 1244 by by IN 1245 1252 p50/p50 p50/p50 NN 1253 1263 homodimers homodimer NNS 1263 1264 . . . 1265 1275 CONCLUSION conclusion NN 1275 1276 : : : 1277 1284 Overall overall JJ 1284 1285 , , , 1286 1289 the the DT 1290 1297 results result NNS 1298 1305 suggest suggest VBP 1306 1310 that that IN 1311 1319 NF-kappa NF-kappa NNP 1320 1321 B B NNP 1322 1325 may may MD 1326 1330 play play VB 1331 1332 a a DT 1333 1340 central central JJ 1341 1345 part part NN 1346 1348 in in IN 1349 1352 the the DT 1353 1363 activation activation NN 1364 1366 of of IN 1367 1379 infiltrating infiltrate VBG 1380 1381 T t NN 1382 1387 cells cell NNS 1388 1390 in in IN 1391 1398 chronic chronic JJ 1399 1409 rheumatoid rheumatoid JJ 1410 1419 arthritis arthritis NN 1419 1420 . . . 1421 1424 The the DT 1425 1435 activation activation NN 1436 1438 of of IN 1439 1443 this this DT 1444 1451 nuclear nuclear JJ 1452 1458 factor factor NN 1459 1461 is be VBZ 1462 1475 qualitatively qualitatively RB 1476 1485 different different JJ 1486 1488 in in IN 1489 1499 rheumatoid rheumatoid JJ 1500 1508 synovial synovial JJ 1509 1510 T t NN 1511 1516 cells cell NNS 1517 1519 to to TO 1520 1524 that that DT 1525 1527 in in IN 1528 1533 other other JJ 1534 1539 forms form NNS 1540 1542 of of IN 1543 1557 non-rheumatoid non-rheumatoid JJ 1558 1567 arthritis arthritis NN 1568 1569 ( ( ( 1569 1572 for for IN 1573 1580 example example NN 1580 1581 , , , 1582 1596 osteoarthritis osteoarthritis NN 1596 1597 , , , 1598 1619 spondyloarthropathies spondyloarthropathy NNS 1619 1620 ) ) ) 1620 1621 . . .