0 15 Phosphorylation phosphorylation NN 16 18 of of IN 19 24 TRAF2 TRAF2 NNP 25 33 inhibits inhibit VBZ 34 41 binding bind VBG 42 44 to to TO 45 48 the the DT 49 53 CD40 CD40 NNP 54 65 cytoplasmic cytoplasmic JJ 66 72 domain domain NN 72 73 . . . 75 80 TRAF2 TRAF2 NNP 81 83 is be VBZ 84 85 a a DT 86 92 signal signal NN 93 104 transducing transducing NN 105 112 adaptor adaptor NN 113 121 molecule molecule NN 122 127 which which WDT 128 133 binds bind VBZ 134 136 to to TO 137 140 the the DT 141 145 CD40 CD40 NNP 146 157 cytoplasmic cytoplasmic JJ 158 164 domain domain NN 164 165 . . . 166 168 We we PRP 169 173 have have VBP 174 179 found find VBN 180 184 that that IN 185 187 it it PRP 188 190 is be VBZ 191 205 phosphorylated phosphorylate VBN 205 206 , , , 207 220 predominantly predominantly RB 221 223 on on IN 224 230 serine serine NN 231 239 residues residue NNS 239 240 , , , 241 245 when when WRB 246 257 transiently transiently RB 258 271 overexpressed overexpresse VBN 272 274 in in IN 275 278 293 293 CD 279 284 cells cell NNS 284 285 . . . 286 289 The the DT 290 305 phosphorylation phosphorylation NN 306 313 appears appear VBZ 314 316 to to TO 317 319 be be VB 320 327 related related JJ 328 330 to to TO 331 334 the the DT 335 344 signaling signaling NN 345 351 events event NNS 352 356 that that WDT 357 360 are be VBP 361 370 activated activate VBN 371 373 by by IN 374 379 TRAF2 traf2 NN 380 385 under under IN 386 391 these these DT 392 405 circumstances circumstance NNS 405 406 , , , 407 412 since since IN 413 416 two two CD 417 430 nonfunctional nonfunctional JJ 431 438 mutants mutant NNS 439 443 were be VBD 444 449 found find VBN 450 452 to to TO 453 455 be be VB 456 470 phosphorylated phosphorylate VBN 471 484 significantly significantly RB 485 489 less less JJR 490 494 than than IN 495 498 the the DT 499 508 wild-type wild-type JJ 509 516 protein protein NN 516 517 . . . 518 529 Furthermore furthermore RB 529 530 , , , 531 534 the the DT 535 550 phosphorylation phosphorylation NN 551 557 status status NN 558 560 of of IN 561 566 TRAF2 TRAF2 NNP 567 570 had have VBD 571 582 significant significant JJ 583 590 effects effect NNS 591 593 on on IN 594 597 the the DT 598 605 ability ability NN 606 608 of of IN 609 612 the the DT 613 620 protein protein NN 621 623 to to TO 624 628 bind bind VB 629 631 to to TO 632 636 CD40 CD40 NNP 636 637 , , , 638 640 as as IN 641 650 evidenced evidence VBN 651 653 by by IN 654 657 our our PRP$ 658 670 observations observation NNS 671 675 that that IN 676 679 the the DT 680 684 CD40 CD40 NNP 685 696 cytoplasmic cytoplasmic JJ 697 703 domain domain NN 704 714 interacted interact VBD 715 729 preferentially preferentially RB 730 734 with with IN 735 754 underphosphorylated underphosphorylate VBN 755 760 TRAF2 traf2 NN 761 764 and and CC 765 769 that that IN 770 781 phosphatase phosphatase NN 782 791 treatment treatment NN 792 805 significantly significantly RB 806 814 enhanced enhance VBD 815 818 the the DT 819 826 binding binding NN 827 829 of of IN 830 835 TRAF2 traf2 NN 836 838 to to TO 839 843 CD40 CD40 NNP 843 844 . . . 845 847 We we PRP 848 856 conclude conclude VBP 857 861 from from IN 862 867 these these DT 868 875 studies study NNS 876 880 that that IN 881 884 the the DT 885 900 phosphorylation phosphorylation NN 901 903 of of IN 904 909 TRAF2 traf2 NN 910 912 is be VBZ 913 919 likely likely JJ 920 922 to to TO 923 927 play play VB 928 930 an an DT 931 940 important important JJ 941 945 role role NN 946 948 in in IN 949 959 regulating regulate VBG 960 969 signaling signaling NN 970 972 by by IN 973 979 virtue virtue NN 980 982 of of IN 983 986 its its PRP$ 987 994 ability ability NN 995 997 to to TO 998 1007 influence influence VB 1008 1011 the the DT 1012 1022 CD40-TRAF2 cd40-traf2 JJ 1023 1034 interaction interaction NN 1034 1035 . . . 1036 1045 Copyright Copyright NNP 1046 1050 1999 1999 CD 1051 1059 Academic Academic NNP 1060 1065 Press Press NNP 1065 1066 . . .