0	8	Cellular	cellular	JJ
9	20	disposition	disposition	NN
21	23	of	of	IN
24	41	sulphamethoxazole	sulphamethoxazole	NN
42	45	and	and	CC
46	49	its	its	PRP$
50	61	metabolites	metabolite	NNS
61	62	:	:	:
63	75	implications	implication	NNS
76	79	for	for	IN
80	96	hypersensitivity	hypersensitivity	NN
96	97	.	.	.

99	100	1	1	LS
100	101	.	.	.

102	115	Bioactivation	Bioactivation	NNP
116	118	of	of	IN
119	136	sulphamethoxazole	sulphamethoxazole	NN
137	138	(	(	(
138	141	SMX	SMX	NNP
141	142	)	)	)
143	145	to	to	TO
146	165	chemically-reactive	chemically-reactive	JJ
166	177	metabolites	metabolite	NNS
178	181	and	and	CC
182	192	subsequent	subsequent	JJ
193	200	protein	protein	NN
201	212	conjugation	conjugation	NN
213	215	is	be	VBZ
216	223	thought	think	VBN
224	226	to	to	TO
227	229	be	be	VB
230	238	involved	involve	VBN
239	241	in	in	IN
242	245	SMX	SMX	NNP
246	262	hypersensitivity	hypersensitivity	NN
262	263	.	.	.

264	266	We	we	PRP
267	271	have	have	VBP
272	281	therefore	therefore	RB
282	290	examined	examine	VBN
291	294	the	the	DT
295	303	cellular	cellular	JJ
304	314	metabolism	metabolism	NN
314	315	,	,	,
316	327	disposition	disposition	NN
328	331	and	and	CC
332	343	conjugation	conjugation	NN
344	346	of	of	IN
347	350	SMX	SMX	NNP
351	354	and	and	CC
355	358	its	its	PRP$
359	370	metabolites	metabolite	NNS
371	373	in	in	FW
374	379	vitro	vitro	FW
379	380	.	.	.

381	382	2	2	LS
382	383	.	.	.

384	388	Flow	Flow	NNP
389	398	cytometry	cytometry	NN
399	407	revealed	reveal	VBD
408	415	binding	binding	NN
416	418	of	of	IN
419	428	N-hydroxy	N-hydroxy	NNP
429	430	(	(	(
430	438	SMX-NHOH	SMX-NHOH	NNP
438	439	)	)	)
440	443	and	and	CC
444	451	nitroso	nitroso	NN
452	453	(	(	(
453	459	SMX-NO	smx-no	NN
459	460	)	)	)
461	472	metabolites	metabolite	NNS
473	475	of	of	IN
476	479	SMX	SMX	NNP
479	480	,	,	,
481	484	but	but	CC
485	488	not	not	RB
489	491	of	of	IN
492	495	SMX	SMX	NNP
496	502	itself	itself	PRP
502	503	,	,	,
504	506	to	to	TO
507	510	the	the	DT
511	518	surface	surface	NN
519	521	of	of	IN
522	528	viable	viable	JJ
529	534	white	white	JJ
535	540	blood	blood	NN
541	546	cells	cell	NNS
546	547	.	.	.

548	556	Cellular	cellular	JJ
557	568	haptenation	haptenation	NN
569	571	by	by	IN
572	578	SMX-NO	SMX-NO	NNP
579	582	was	be	VBD
583	590	reduced	reduce	VBN
591	593	by	by	IN
594	603	exogenous	exogenous	JJ
604	615	glutathione	glutathione	NN
616	617	(	(	(
617	620	GSH	GSH	NNP
620	621	)	)	)
621	622	.	.	.

623	624	3	3	LS
624	625	.	.	.

626	634	SMX-NHOH	SMX-NHOH	NNP
635	638	and	and	CC
639	645	SMX-NO	SMX-NO	NNP
646	650	were	be	VBD
651	658	rapidly	rapidly	RB
659	666	reduced	reduce	VBN
667	671	back	back	RB
672	674	to	to	TO
675	678	the	the	DT
679	685	parent	parent	NN
686	694	compound	compound	NN
695	697	by	by	IN
698	706	cysteine	cysteine	NN
707	708	(	(	(
708	711	CYS	CYS	NNP
711	712	)	)	)
712	713	,	,	,
714	717	GSH	GSH	NNP
717	718	,	,	,
719	724	human	human	JJ
725	735	peripheral	peripheral	JJ
736	741	blood	blood	NN
742	747	cells	cell	NNS
748	751	and	and	CC
752	758	plasma	plasma	NN
758	759	,	,	,
760	770	suggesting	suggest	VBG
771	775	that	that	IN
776	780	this	this	DT
781	783	is	be	VBZ
784	786	an	an	DT
787	796	important	important	JJ
797	800	and	and	CC
801	811	ubiquitous	ubiquitous	JJ
812	827	bioinactivation	bioinactivation	NN
828	837	mechanism	mechanism	NN
837	838	.	.	.

839	840	4	4	LS
840	841	.	.	.

842	854	Fluorescence	Fluorescence	NNP
855	859	HPLC	HPLC	NNP
860	866	showed	show	VBD
867	871	that	that	IN
872	880	SMX-NHOH	SMX-NHOH	NNP
881	884	and	and	CC
885	891	SMX-NO	SMX-NO	NNP
892	900	depleted	deplete	VBD
901	904	CYS	cys	NN
905	908	and	and	CC
909	912	GSH	gsh	NN
913	915	in	in	IN
916	922	buffer	buffer	NN
922	923	,	,	,
924	927	and	and	CC
928	930	to	to	TO
931	932	a	a	DT
933	939	lesser	less	JJR
940	946	extent	extent	NN
946	947	,	,	,
948	950	in	in	IN
951	956	cells	cell	NNS
957	960	and	and	CC
961	967	plasma	plasma	NN
967	968	.	.	.

969	970	5	5	LS
970	971	.	.	.

972	982	Neutrophil	Neutrophil	NNP
983	992	apoptosis	apoptosis	NN
993	996	and	and	CC
997	1007	inhibition	inhibition	NN
1008	1010	of	of	IN
1011	1021	neutrophil	neutrophil	NN
1022	1030	function	function	NN
1031	1035	were	be	VBD
1036	1043	induced	induce	VBN
1044	1046	at	at	IN
1047	1052	lower	low	JJR
1053	1067	concentrations	concentration	NNS
1068	1070	of	of	IN
1071	1079	SMX-NHOH	SMX-NHOH	NNP
1080	1083	and	and	CC
1084	1090	SMX-NO	SMX-NO	NNP
1091	1095	than	than	IN
1096	1101	those	those	DT
1102	1110	inducing	induce	VBG
1111	1115	loss	loss	NN
1116	1118	of	of	IN
1119	1127	membrane	membrane	NN
1128	1137	viability	viability	NN
1137	1138	,	,	,
1139	1143	with	with	IN
1144	1147	SMX	SMX	NNP
1148	1154	having	have	VBG
1155	1157	no	no	DT
1158	1164	effect	effect	NN
1164	1165	.	.	.

1166	1177	Lymphocytes	Lymphocyte	NNP
1178	1182	were	be	VBD
1183	1196	significantly	significantly	RB
1197	1198	(	(	(
1198	1204	P<0.05	p<0.05	NN
1204	1205	)	)	)
1206	1210	more	more	RBR
1211	1220	sensitive	sensitive	JJ
1221	1223	to	to	TO
1224	1227	the	the	DT
1228	1234	direct	direct	JJ
1235	1244	cytotoxic	cytotoxic	JJ
1245	1252	effects	effect	NNS
1253	1255	of	of	IN
1256	1262	SMX-NO	SMX-NO	NNP
1263	1267	than	than	IN
1268	1279	neutrophils	neutrophil	NNS
1279	1280	.	.	.

1281	1282	6	6	CD
1282	1283	.	.	.

1284	1296	Partitioning	partitioning	NN
1297	1299	of	of	IN
1300	1308	SMX-NHOH	SMX-NHOH	NNP
1309	1313	into	into	IN
1314	1317	red	red	JJ
1318	1323	blood	blood	NN
1324	1329	cells	cell	NNS
1330	1333	was	be	VBD
1334	1347	significantly	significantly	RB
1348	1349	(	(	(
1349	1355	P<0.05	p<0.05	NN
1355	1356	)	)	)
1357	1362	lower	low	JJR
1363	1367	than	than	IN
1368	1372	with	with	IN
1373	1376	the	the	DT
1377	1390	hydroxylamine	hydroxylamine	NN
1391	1393	of	of	IN
1394	1401	dapsone	dapsone	NN
1401	1402	.	.	.

1403	1404	7	7	CD
1404	1405	.	.	.

1406	1409	Our	our	PRP$
1410	1417	results	result	NNS
1418	1425	suggest	suggest	VBP
1426	1430	that	that	IN
1431	1434	the	the	DT
1435	1442	balance	balance	NN
1443	1450	between	between	IN
1451	1460	oxidation	oxidation	NN
1461	1463	of	of	IN
1464	1467	SMX	SMX	NNP
1468	1470	to	to	TO
1471	1474	its	its	PRP$
1475	1480	toxic	toxic	JJ
1481	1492	metabolites	metabolite	NNS
1493	1496	and	and	CC
1497	1502	their	their	PRP$
1503	1512	reduction	reduction	NN
1513	1515	is	be	VBZ
1516	1518	an	an	DT
1519	1528	important	important	JJ
1529	1539	protective	protective	JJ
1540	1548	cellular	cellular	JJ
1549	1558	mechanism	mechanism	NN
1558	1559	.	.	.

1560	1562	If	if	IN
1563	1565	an	an	DT
1566	1575	imbalance	imbalance	NN
1576	1582	exists	exist	VBZ
1582	1583	,	,	,
1584	1595	haptenation	haptenation	NN
1596	1598	of	of	IN
1599	1602	the	the	DT
1603	1608	toxic	toxic	JJ
1609	1620	metabolites	metabolite	NNS
1621	1623	to	to	TO
1624	1630	bodily	bodily	VB
1631	1639	proteins	protein	NNS
1640	1649	including	include	VBG
1650	1653	the	the	DT
1654	1661	surface	surface	NN
1662	1664	of	of	IN
1665	1671	viable	viable	JJ
1672	1677	cells	cell	NNS
1678	1681	can	can	MD
1682	1687	occur	occur	VB
1687	1688	,	,	,
1689	1692	and	and	CC
1693	1696	may	may	MD
1697	1703	result	result	VB
1704	1706	in	in	IN
1707	1711	drug	drug	NN
1712	1728	hypersensitivity	hypersensitivity	NN
1728	1729	.	.	.