0	15	Transcriptional	transcriptional	JJ
16	25	targeting	targeting	NN
26	28	of	of	IN
29	39	retroviral	retroviral	JJ
40	47	vectors	vector	NNS
48	50	to	to	TO
51	54	the	the	DT
55	69	erythroblastic	erythroblastic	JJ
70	77	progeny	progeny	NN
78	80	of	of	IN
81	91	transduced	transduce	VBN
92	105	hematopoietic	hematopoietic	JJ
106	110	stem	stem	NN
111	116	cells	cell	NNS
116	117	.	.	.

119	127	Targeted	target	VBN
128	138	expression	expression	NN
139	141	to	to	TO
142	150	specific	specific	JJ
151	158	tissues	tissue	NNS
159	161	or	or	CC
162	166	cell	cell	NN
167	175	lineages	lineage	NNS
176	178	is	be	VBZ
179	180	a	a	DT
181	190	necessary	necessary	JJ
191	198	feature	feature	NN
199	201	of	of	IN
202	203	a	a	DT
204	208	gene	gene	NN
209	216	therapy	therapy	NN
217	223	vector	vector	NN
224	227	for	for	IN
228	232	many	many	JJ
233	241	clinical	clinical	JJ
242	254	applications	application	NNS
254	255	,	,	,
256	260	such	such	JJ
261	263	as	as	IN
264	274	correction	correction	NN
275	277	of	of	IN
278	296	hemoglobinopathies	hemoglobinopathy	NNS
297	299	or	or	CC
300	312	thalassemias	thalassemia	NNS
313	315	by	by	IN
316	331	transplantation	transplantation	NN
332	334	of	of	IN
335	346	genetically	genetically	RB
347	355	modified	modify	VBN
356	369	hematopoietic	hematopoietic	JJ
370	374	stem	stem	NN
375	380	cells	cell	NNS
380	381	.	.	.

382	384	We	we	PRP
385	394	developed	develop	VBD
395	405	retroviral	retroviral	JJ
406	413	vectors	vector	NNS
414	416	in	in	IN
417	422	which	which	WDT
423	426	the	the	DT
427	439	constitutive	constitutive	JJ
440	445	viral	viral	JJ
446	454	enhancer	enhancer	NN
455	457	in	in	IN
458	461	the	the	DT
462	464	U3	u3	NN
465	471	region	region	NN
472	474	of	of	IN
475	478	the	the	DT
479	480	3	3	CD
480	481	’	'	SYM
482	485	LTR	ltr	NN
486	488	is	be	VBZ
489	497	replaced	replace	VBN
498	500	by	by	IN
501	503	an	an	DT
504	518	autoregulatory	autoregulatory	JJ
519	527	enhancer	enhancer	NN
528	530	of	of	IN
531	534	the	the	DT
535	553	erythroid-specific	erythroid-specific	JJ
554	560	GATA-1	gata-1	NN
561	574	transcription	transcription	NN
575	581	factor	factor	NN
582	586	gene	gene	NN
586	587	.	.	.

588	591	The	the	DT
592	600	replaced	replace	VBN
601	609	enhancer	enhancer	NN
610	612	is	be	VBZ
613	623	propagated	propagate	VBN
624	626	to	to	TO
627	630	the	the	DT
631	632	5	5	CD
632	633	’	'	SYM
634	637	LTR	ltr	NN
638	642	upon	upon	IN
643	654	integration	integration	NN
655	659	into	into	IN
660	663	the	the	DT
664	670	target	target	NN
671	675	cell	cell	NN
676	682	genome	genome	NN
682	683	.	.	.

684	687	The	the	DT
688	696	modified	modify	VBN
697	704	vectors	vector	NNS
705	709	were	be	VBD
710	714	used	use	VBN
715	717	to	to	TO
718	727	transduce	transduce	VB
728	733	human	human	JJ
734	747	hematopoietic	hematopoietic	JJ
748	752	cell	cell	NN
753	758	lines	line	NNS
758	759	,	,	,
760	764	cord	cord	NN
765	778	blood-derived	blood-derived	JJ
779	786	CD34(+)	cd34(+)	JJ
787	802	stem/progenitor	stem/progenitor	NN
803	808	cells	cell	NNS
808	809	,	,	,
810	813	and	and	CC
814	820	murine	murine	JJ
821	825	bone	bone	NN
826	832	marrow	marrow	NN
833	845	repopulating	repopulate	VBG
846	850	stem	stem	NN
851	856	cells	cell	NNS
856	857	.	.	.

858	861	The	the	DT
862	872	expression	expression	NN
873	875	of	of	IN
876	887	appropriate	appropriate	JJ
888	896	reporter	reporter	NN
897	902	genes	gene	NNS
903	904	(	(	(
904	912	triangle	triangle	NN
913	920	upLNGFR	upLNGFR	NNP
920	921	,	,	,
922	926	EGFP	EGFP	NNP
926	927	)	)	)
928	931	was	be	VBD
932	940	analyzed	analyze	VBN
941	943	in	in	IN
944	947	the	the	DT
948	962	differentiated	differentiate	VBN
963	970	progeny	progeny	NN
971	973	of	of	IN
974	984	transduced	transduce	VBN
985	989	stem	stem	NN
990	995	cells	cell	NNS
996	998	in	in	FW
999	1004	vitro	vitro	FW
1004	1005	,	,	,
1006	1008	in	in	IN
1009	1015	liquid	liquid	JJ
1016	1023	culture	culture	NN
1024	1026	as	as	RB
1027	1031	well	well	RB
1032	1034	as	as	IN
1035	1037	in	in	IN
1038	1048	clonogenic	clonogenic	JJ
1049	1054	assay	assay	NN
1054	1055	,	,	,
1056	1059	and	and	CC
1060	1062	in	in	FW
1063	1067	vivo	vivo	FW
1067	1068	,	,	,
1069	1074	after	after	IN
1075	1079	bone	bone	NN
1080	1086	marrow	marrow	NN
1087	1102	transplantation	transplantation	NN
1103	1105	in	in	IN
1106	1114	lethally	lethally	RB
1115	1125	irradiated	irradiated	JJ
1126	1130	mice	mouse	NNS
1130	1131	.	.	.

1132	1135	The	the	DT
1136	1142	GATA-1	gata-1	NN
1143	1157	autoregulatory	autoregulatory	JJ
1158	1166	enhancer	enhancer	NN
1167	1178	effectively	effectively	RB
1179	1188	restricts	restrict	VBZ
1189	1192	the	the	DT
1193	1203	expression	expression	NN
1204	1206	of	of	IN
1207	1210	the	the	DT
1211	1221	LTR-driven	ltr-driven	JJ
1222	1230	proviral	proviral	JJ
1231	1244	transcription	transcription	NN
1245	1249	unit	unit	NN
1250	1252	to	to	TO
1253	1256	the	the	DT
1257	1271	erythroblastic	erythroblastic	JJ
1272	1279	progeny	progeny	NN
1280	1282	of	of	IN
1283	1287	both	both	CC
1288	1293	human	human	JJ
1294	1305	progenitors	progenitor	NNS
1306	1309	and	and	CC
1310	1328	mouse-repopulating	mouse-repopulating	JJ
1329	1333	stem	stem	NN
1334	1339	cells	cell	NNS
1339	1340	.	.	.

1341	1350	Packaging	packaging	NN
1351	1353	of	of	IN
1354	1359	viral	viral	JJ
1360	1369	particles	particle	NNS
1369	1370	,	,	,
1371	1382	integration	integration	NN
1383	1387	into	into	IN
1388	1391	the	the	DT
1392	1398	target	target	NN
1399	1405	genome	genome	NN
1405	1406	,	,	,
1407	1410	and	and	CC
1411	1420	stability	stability	NN
1421	1423	of	of	IN
1424	1427	the	the	DT
1428	1438	integrated	integrate	VBN
1439	1447	provirus	provirus	NN
1448	1451	are	be	VBP
1452	1455	not	not	RB
1456	1464	affected	affect	VBN
1465	1467	by	by	IN
1468	1471	the	the	DT
1472	1475	LTR	LTR	NNP
1476	1488	modification	modification	NN
1488	1489	.	.	.

1490	1498	Enhancer	Enhancer	NNP
1499	1510	replacement	replacement	NN
1511	1513	is	be	VBZ
1514	1523	therefore	therefore	RB
1524	1526	an	an	DT
1527	1536	effective	effective	JJ
1537	1545	strategy	strategy	NN
1546	1548	to	to	TO
1549	1555	target	target	VB
1556	1566	expression	expression	NN
1567	1569	of	of	IN
1570	1571	a	a	DT
1572	1582	retroviral	retroviral	JJ
1583	1592	transgene	transgene	NN
1593	1595	to	to	TO
1596	1597	a	a	DT
1598	1606	specific	specific	JJ
1607	1614	progeny	progeny	NN
1615	1617	of	of	IN
1618	1628	transduced	transduce	VBN
1629	1642	hematopoietic	hematopoietic	JJ
1643	1647	stem	stem	NN
1648	1653	cells	cell	NNS
1653	1654	.	.	.