0 4 Cell cell NN 5 21 growth-regulated growth-regulated JJ 22 32 expression expression NN 33 35 of of IN 36 45 mammalian mammalian JJ 46 50 MCM5 mcm5 NN 51 54 and and CC 55 59 MCM6 mcm6 NN 60 65 genes gene NNS 66 74 mediated mediate VBN 75 77 by by IN 78 81 the the DT 82 95 transcription transcription NN 96 102 factor factor NN 103 107 E2F. E2F. NNP 109 119 Initiation Initiation NNP 120 122 of of IN 123 126 DNA DNA NNP 127 138 replication replication NN 139 147 requires require VBZ 148 151 the the DT 152 160 function function NN 161 163 of of IN 164 167 MCM MCM NNP 168 172 gene gene NN 173 181 products product NNS 181 182 , , , 183 188 which which WDT 189 200 participate participate VBP 201 203 in in IN 204 212 ensuring ensure VBG 213 217 that that IN 218 221 DNA dna NN 222 233 replication replication NN 234 240 occurs occur VBZ 241 245 only only RB 246 250 once once RB 251 253 in in IN 254 257 the the DT 258 262 cell cell NN 263 268 cycle cycle NN 268 269 . . . 270 280 Expression expression NN 281 283 of of IN 284 287 all all DT 288 297 mammalian mammalian JJ 298 303 genes gene NNS 304 306 of of IN 307 310 the the DT 311 314 MCM MCM NNP 315 321 family family NN 322 324 is be VBZ 325 332 induced induce VBN 333 335 by by IN 336 342 growth growth NN 343 354 stimulation stimulation NN 354 355 , , , 356 362 unlike unlike IN 363 368 yeast yeast NN 368 369 , , , 370 373 and and CC 374 377 the the DT 378 382 mRNA mrna NN 383 389 levels level NNS 390 394 peak peak VBP 395 397 at at IN 398 402 G1/S G1/S NNP 403 411 boundary boundary NN 411 412 . . . 413 415 In in IN 416 420 this this DT 421 426 study study NN 426 427 , , , 428 430 we we PRP 431 439 examined examine VBD 440 443 the the DT 444 459 transcriptional transcriptional JJ 460 470 activities activity NNS 471 473 of of IN 474 482 isolated isolate VBN 483 488 human human JJ 489 492 MCM MCM NNP 493 497 gene gene NN 498 507 promoters promoter NNS 507 508 . . . 509 514 Human human JJ 515 519 MCM5 MCM5 NNP 520 523 and and CC 524 528 MCM6 MCM6 NNP 529 538 promoters promoter NNS 539 543 with with IN 544 552 mutation mutation NN 553 555 in in IN 556 559 the the DT 560 563 E2F E2F NNP 564 569 sites site NNS 570 576 failed fail VBD 577 579 in in IN 580 588 promoter promoter NN 589 599 regulation regulation NN 600 609 following follow VBG 610 615 serum serum NN 616 627 stimulation stimulation NN 628 631 and and CC 632 641 exogenous exogenous JJ 642 645 E2F e2f NN 646 656 expression expression NN 656 657 . . . 658 660 In in IN 661 669 addition addition NN 669 670 , , , 671 673 we we PRP 674 684 identified identify VBD 685 686 a a DT 687 692 novel novel JJ 693 701 E2F-like e2f-like JJ 702 710 sequence sequence NN 711 713 in in IN 714 719 human human JJ 720 724 MCM6 mcm6 NN 725 733 promoter promoter NN 734 739 which which WDT 740 750 cooperates cooperate VBZ 751 755 with with IN 756 759 the the DT 760 769 authentic authentic JJ 770 773 E2F e2f NN 774 779 sites site NNS 780 782 in in IN 783 796 E2F-dependent e2f-dependent JJ 797 807 regulation regulation NN 807 808 . . . 809 815 Forced force VBN 816 826 expression expression NN 827 829 of of IN 830 834 E2F1 E2F1 NNP 835 840 could could MD 841 847 induce induce VB 848 858 expression expression NN 859 861 of of IN 862 865 all all DT 866 873 members member NNS 874 876 of of IN 877 880 the the DT 881 891 endogenous endogenous JJ 892 895 MCM mcm NN 896 901 genes gene NNS 902 904 in in IN 905 908 rat rat NN 909 918 embryonal embryonal JJ 919 929 fibroblast fibroblast NN 930 935 REF52 ref52 NN 936 941 cells cell NNS 941 942 . . . 943 946 Our our PRP$ 947 954 results result NNS 955 967 demonstrated demonstrate VBD 968 972 that that IN 973 976 the the DT 977 993 growth-regulated growth-regulated JJ 994 1004 expression expression NN 1005 1007 of of IN 1008 1017 mammalian mammalian JJ 1018 1022 MCM5 mcm5 NN 1023 1026 and and CC 1027 1031 MCM6 mcm6 NN 1032 1037 genes gene NNS 1037 1038 , , , 1039 1042 and and CC 1043 1053 presumably presumably RB 1054 1059 other other JJ 1060 1063 MCM MCM NNP 1064 1071 members member NNS 1071 1072 , , , 1073 1075 is be VBZ 1076 1085 primarily primarily RB 1086 1095 regulated regulate VBN 1096 1098 by by IN 1099 1102 E2F E2F NNP 1103 1110 through through IN 1111 1118 binding binding NN 1119 1121 to to TO 1122 1130 multiple multiple JJ 1131 1134 E2F e2f NN 1135 1140 sites site NNS 1141 1143 in in IN 1144 1147 the the DT 1148 1157 promoters promoter NNS 1157 1158 . . .