0 15 Transcriptional transcriptional JJ 16 23 control control NN 24 26 of of IN 27 30 the the DT 31 35 IL-5 il-5 NN 36 40 gene gene NN 41 43 by by IN 44 49 human human JJ 50 56 helper helper NN 57 58 T t NN 59 64 cells cell NNS 64 65 : : : 66 70 IL-5 IL-5 NNP 71 80 synthesis synthesis NN 81 83 is be VBZ 84 93 regulated regulate VBN 94 107 independently independently RB 108 112 from from IN 113 117 IL-2 il-2 NN 118 120 or or CC 121 125 IL-4 il-4 NN 126 135 synthesis synthesis NN 135 136 . . . 138 148 BACKGROUND background NN 148 149 : : : 150 154 IL-5 IL-5 NNP 155 157 is be VBZ 158 171 fundamentally fundamentally RB 172 180 involved involve VBN 181 183 in in IN 184 196 eosinophilic eosinophilic JJ 197 209 inflammation inflammation NN 209 210 . . . 211 218 Control Control NNP 219 221 of of IN 222 226 IL-5 IL-5 NNP 227 237 production production NN 238 241 may may MD 242 244 be be VB 245 254 effective effective JJ 255 258 for for IN 259 262 the the DT 263 273 management management NN 274 276 of of IN 277 285 allergic allergic JJ 286 294 diseases disease NNS 294 295 . . . 296 305 OBJECTIVE objective NN 305 306 : : : 307 309 We we PRP 310 315 aimed aim VBD 316 318 to to TO 319 323 find find VB 324 327 the the DT 328 343 transcriptional transcriptional JJ 344 354 mechanisms mechanism NNS 355 359 that that WDT 360 368 regulate regulate VBP 369 372 the the DT 373 377 IL-5 il-5 NN 378 382 gene gene NN 383 385 to to TO 386 397 selectively selectively RB 398 405 control control VB 406 410 IL-5 il-5 NN 411 420 synthesis synthesis NN 420 421 . . . 422 429 METHODS methods NNS 429 430 : : : 431 448 Allergen-specific allergen-specific JJ 449 455 T-cell t-cell NN 456 462 clones clone NNS 463 466 and and CC 467 473 T-cell t-cell NN 474 484 hybridomas hybridoma NNS 485 489 were be VBD 490 501 established establish VBN 502 506 from from IN 507 510 the the DT 511 521 peripheral peripheral JJ 522 527 blood blood NN 528 539 lymphocytes lymphocyte NNS 540 542 of of IN 543 551 patients patient NNS 552 556 with with IN 557 563 asthma asthma NN 563 564 , , , 565 568 and and CC 569 572 the the DT 573 588 transcriptional transcriptional JJ 589 599 regulation regulation NN 600 602 of of IN 603 606 the the DT 607 611 IL-5 IL-5 NNP 612 616 gene gene NN 617 620 was be VBD 621 633 investigated investigate VBN 634 638 with with IN 639 648 transient transient JJ 649 661 transfection transfection NN 662 665 and and CC 666 681 electrophoretic electrophoretic JJ 682 690 mobility mobility NN 691 696 shift shift NN 697 705 analysis analysis NN 705 706 . . . 707 714 RESULTS results NNS 714 715 : : : 716 717 A a DT 718 723 human human JJ 724 728 IL-5 il-5 NN 729 757 promoter/enhancer-luciferase promoter/enhancer-luciferase NN 758 762 gene gene NN 763 772 construct construct NN 772 773 , , , 774 788 pIL-5(-511)Luc pil-5(-511)luc NN 788 789 , , , 790 793 was be VBD 794 805 transcribed transcribe VBN 806 808 on on IN 809 819 activation activation NN 820 822 of of IN 823 837 IL-5-producing il-5-producing JJ 838 844 T-cell t-cell NN 845 851 clones clone NNS 851 852 , , , 853 856 but but CC 857 860 not not RB 861 878 IL-5-nonproducing il-5-nonproducing JJ 879 886 clones. clones. NN 887 901 pIL-5(-511)Luc pil-5(-511)luc NN 902 905 was be VBD 906 917 transcribed transcribe VBN 918 920 by by IN 921 927 T-cell t-cell NN 928 938 hybridomas hybridoma NNS 939 946 derived derive VBN 947 951 from from IN 952 958 fusion fusion NN 959 966 between between IN 967 981 IL-5-producing il-5-producing JJ 982 988 T-cell t-cell NN 989 995 clones clone NNS 996 999 and and CC 1000 1002 an an DT 1003 1007 IL-5 il-5 NN 1008 1026 gene-nonexpressing gene-nonexpressing JJ 1027 1033 T-cell t-cell NN 1034 1038 line line NN 1038 1039 , , , 1040 1043 but but CC 1044 1047 not not RB 1048 1050 by by IN 1051 1061 hybridomas hybridoma NNS 1062 1069 derived derive VBN 1070 1074 from from IN 1075 1092 IL-5-nonproducing il-5-nonproducing JJ 1093 1099 T-cell t-cell NN 1100 1106 clones clone NNS 1106 1107 . . . 1108 1112 IL-5 IL-5 NNP 1113 1122 synthesis synthesis NN 1123 1126 was be VBD 1127 1130 not not RB 1131 1135 only only RB 1136 1143 induced induce VBN 1144 1146 by by IN 1147 1153 T-cell t-cell NN 1154 1162 receptor receptor NN 1163 1174 stimulation stimulation NN 1175 1178 but but CC 1179 1183 also also RB 1184 1186 by by IN 1187 1191 IL-2 il-2 NN 1192 1200 receptor receptor NN 1201 1212 stimulation stimulation NN 1212 1213 . . . 1214 1221 Binding binding NN 1222 1224 of of IN 1225 1230 NF-AT NF-AT NNP 1230 1231 , , , 1232 1241 NF-kappaB NF-kappaB NNP 1241 1242 , , , 1243 1246 and and CC 1247 1251 AP-1 ap-1 NN 1252 1255 was be VBD 1256 1263 induced induce VBN 1264 1266 by by IN 1267 1273 T-cell t-cell NN 1274 1282 receptor receptor NN 1283 1284 ( ( ( 1284 1287 TcR TcR NNP 1287 1288 ) ) ) 1289 1300 stimulation stimulation NN 1300 1301 , , , 1302 1310 although although IN 1311 1316 there there EX 1317 1320 was be VBD 1321 1323 no no DT 1324 1335 significant significant JJ 1336 1348 upregulation upregulation NN 1349 1351 of of IN 1352 1359 binding binding NN 1360 1362 by by IN 1363 1367 IL-2 il-2 NN 1368 1379 stimulation stimulation NN 1379 1380 . . . 1381 1391 CONCLUSION conclusion NN 1391 1392 : : : 1393 1397 IL-5 IL-5 NNP 1398 1407 synthesis synthesis NN 1408 1410 by by IN 1411 1416 human human JJ 1417 1423 helper helper NN 1424 1425 T t NN 1426 1431 cells cell NNS 1432 1434 is be VBZ 1435 1444 regulated regulate VBN 1445 1447 at at IN 1448 1451 the the DT 1452 1467 transcriptional transcriptional JJ 1468 1473 level level NN 1473 1474 . . . 1475 1476 A a DT 1477 1483 unique unique JJ 1484 1499 transcriptional transcriptional JJ 1500 1509 mechanism mechanism NN 1510 1518 distinct distinct JJ 1519 1523 from from IN 1524 1529 those those DT 1530 1540 regulating regulate VBG 1541 1544 the the DT 1545 1549 IL-2 il-2 NN 1550 1552 or or CC 1553 1557 IL-4 il-4 NN 1558 1563 genes gene NNS 1564 1569 seems seem VBZ 1570 1572 to to TO 1573 1580 control control VB 1581 1584 the the DT 1585 1589 IL-5 il-5 NN 1590 1594 gene gene NN 1594 1595 . . . 1596 1605 Selective selective JJ 1606 1616 regulation regulation NN 1617 1619 of of IN 1620 1624 IL-5 IL-5 NNP 1625 1629 gene gene NN 1630 1643 transcription transcription NN 1644 1647 may may MD 1648 1650 be be VB 1651 1657 useful useful JJ 1658 1661 for for IN 1662 1670 treating treat VBG 1671 1682 eosinophlic eosinophlic JJ 1683 1695 inflammation inflammation NN 1695 1696 . . .