0 10 Regulation regulation NN 11 13 of of IN 14 17 low low JJ 18 23 shear shear NN 24 36 flow-induced flow-induced JJ 37 41 HAEC HAEC NNP 42 48 VCAM-1 vcam-1 NN 49 59 expression expression NN 60 63 and and CC 64 72 monocyte monocyte NN 73 81 adhesion adhesion NN 81 82 . . . 84 86 We we PRP 87 95 recently recently RB 96 104 reported report VBD 105 109 that that IN 110 119 prolonged prolonged JJ 120 128 exposure exposure NN 129 131 of of IN 132 137 human human JJ 138 144 aortic aortic JJ 145 156 endothelial endothelial JJ 157 162 cells cell NNS 163 164 ( ( ( 164 168 HAEC HAEC NNP 168 169 ) ) ) 170 172 to to TO 173 176 low low JJ 177 182 shear shear NN 183 189 stress stress NN 190 194 flow flow NN 195 203 patterns pattern NNS 204 206 is be VBZ 207 217 associated associate VBN 218 222 with with IN 223 224 a a DT 225 234 sustained sustained JJ 235 243 increase increase NN 244 246 in in IN 247 250 the the DT 251 260 activated activated JJ 261 265 form form NN 266 268 of of IN 269 272 the the DT 273 288 transcriptional transcriptional JJ 289 298 regulator regulator NN 299 306 nuclear nuclear JJ 307 320 factor-kappaB factor-kappaB NNP 321 322 ( ( ( 322 331 NF-kappaB NF-kappaB NNP 331 332 ) ) ) 332 333 . . . 334 338 Here here RB 339 341 we we PRP 342 353 investigate investigate VBP 354 357 the the DT 358 368 hypothesis hypothesis NN 369 373 that that IN 374 377 low low JJ 378 391 shear-induced shear-induced JJ 392 402 activation activation NN 403 405 of of IN 406 415 NF-kappaB NF-kappaB NNP 416 418 is be VBZ 419 430 responsible responsible JJ 431 434 for for IN 435 443 enhanced enhance VBN 444 454 expression expression NN 455 457 of of IN 458 466 vascular vascular JJ 467 471 cell cell NN 472 480 adhesion adhesion NN 481 489 molecule molecule NN 490 491 ( ( ( 491 497 VCAM-1 vcam-1 NN 497 498 ) ) ) 499 508 resulting result VBG 509 511 in in IN 512 521 augmented augmented JJ 522 533 endothelial endothelial JJ 534 547 cell-monocyte cell-monocyte NN 548 549 ( ( ( 549 554 EC-Mn EC-Mn NNP 554 555 ) ) ) 556 564 adhesion adhesion NN 565 568 and and CC 569 573 that that IN 574 578 this this DT 579 589 activation activation NN 590 592 is be VBZ 593 602 dependent dependent JJ 603 605 on on IN 606 619 intracellular intracellular JJ 620 627 oxidant oxidant JJ 628 636 activity activity NN 636 637 . . . 638 644 Before before IN 645 653 exposure exposure NN 654 656 to to TO 657 660 low low JJ 661 666 shear shear NN 667 668 ( ( ( 668 669 2 2 CD 670 677 dyn/cm2 dyn/cm2 NN 677 678 ) ) ) 679 682 for for IN 683 684 6 6 CD 685 686 h h NN 686 687 , , , 688 692 HAEC HAEC NNP 693 697 were be VBD 698 710 preincubated preincubate VBN 711 715 with with IN 716 718 or or CC 719 726 without without IN 727 730 the the DT 731 743 antioxidants antioxidant NNS 744 755 pyrrolidine pyrrolidine NN 756 771 dithiocarbamate dithiocarbamate NN 772 773 ( ( ( 773 777 PDTC PDTC NNP 777 778 ) ) ) 779 781 or or CC 782 801 N-acetyl-L-cysteine N-acetyl-L-cysteine NNP 802 803 ( ( ( 803 806 NAC NAC NNP 806 807 ) ) ) 807 808 . . . 809 813 PDTC PDTC NNP 814 822 strongly strongly RB 823 832 inhibited inhibit VBD 833 836 low low JJ 837 850 shear-induced shear-induced JJ 851 861 activation activation NN 862 864 of of IN 865 874 NF-kappaB NF-kappaB NNP 874 875 , , , 876 886 expression expression NN 887 889 of of IN 890 896 VCAM-1 vcam-1 NN 896 897 , , , 898 901 and and CC 902 907 EC-Mn EC-Mn NNP 908 916 adhesion adhesion NN 916 917 . . . 918 931 Paradoxically paradoxically RB 931 932 , , , 933 936 NAC NAC NNP 937 944 exerted exert VBD 945 946 a a DT 947 955 positive positive JJ 956 962 effect effect NN 963 965 on on IN 966 969 low low JJ 970 983 shear-induced shear-induced JJ 984 990 VCAM-1 vcam-1 NN 991 1001 expression expression NN 1002 1005 and and CC 1006 1011 EC-Mn ec-mn NN 1012 1020 adhesion adhesion NN 1021 1024 and and CC 1025 1029 only only RB 1030 1038 slightly slightly RB 1039 1052 downregulated downregulate VBD 1053 1062 NF-kappaB NF-kappaB NNP 1063 1073 activation activation NN 1073 1074 . . . 1075 1082 However however RB 1082 1083 , , , 1084 1100 cytokine-induced cytokine-induced JJ 1101 1110 NF-kappaB NF-kappaB NNP 1111 1121 activation activation NN 1122 1125 and and CC 1126 1132 VCAM-1 vcam-1 NN 1133 1143 expression expression NN 1144 1147 are be VBP 1148 1155 blocked block VBN 1156 1158 by by IN 1159 1163 both both CC 1164 1168 PDTC PDTC NNP 1169 1172 and and CC 1173 1176 NAC NAC NNP 1176 1177 . . . 1178 1183 These these DT 1184 1188 data datum NNS 1189 1196 suggest suggest VBP 1197 1201 that that IN 1202 1211 NF-kappaB NF-kappaB NNP 1212 1217 plays play VBZ 1218 1219 a a DT 1220 1223 key key JJ 1224 1228 role role NN 1229 1231 in in IN 1232 1235 low low JJ 1236 1249 shear-induced shear-induced JJ 1250 1256 VCAM-1 vcam-1 NN 1257 1267 expression expression NN 1268 1271 and and CC 1272 1276 that that IN 1277 1285 pathways pathway NNS 1286 1295 mediating mediate VBG 1296 1299 low low JJ 1300 1306 shear- shear- NN 1307 1310 and and CC 1311 1327 cytokine-induced cytokine-induced JJ 1328 1333 EC-Mn EC-Mn NNP 1334 1342 adhesion adhesion NN 1343 1346 may may MD 1347 1349 be be VB 1350 1364 differentially differentially RB 1365 1374 regulated regulate VBN 1374 1375 . . .