0 7 Nuclear nuclear JJ 8 20 localization localization NN 21 24 and and CC 25 34 formation formation NN 35 37 of of IN 38 59 beta-catenin-lymphoid beta-catenin-lymphoid JJ 60 68 enhancer enhancer NN 69 75 factor factor NN 76 77 1 1 CD 78 87 complexes complex NNS 88 91 are be VBP 92 95 not not RB 96 106 sufficient sufficient JJ 107 110 for for IN 111 121 activation activation NN 122 124 of of IN 125 129 gene gene NN 130 140 expression expression NN 140 141 . . . 143 145 In in IN 146 154 response response NN 155 157 to to TO 158 168 activation activation NN 169 171 of of IN 172 175 the the DT 176 179 Wnt wnt NN 180 189 signaling signaling NN 190 197 pathway pathway NN 197 198 , , , 199 211 beta-catenin beta-catenin NN 212 223 accumulates accumulate VBZ 224 226 in in IN 227 230 the the DT 231 238 nucleus nucleus NN 238 239 , , , 240 245 where where WRB 246 248 it it PRP 249 259 cooperates cooperate VBZ 260 264 with with IN 265 272 LEF/TCF LEF/TCF NNP 273 274 ( ( ( 274 277 for for IN 278 286 lymphoid lymphoid JJ 287 295 enhancer enhancer NN 296 302 factor factor NN 303 306 and and CC 307 313 T-cell t-cell NN 314 320 factor factor NN 320 321 ) ) ) 322 335 transcription transcription NN 336 343 factors factor NNS 344 346 to to TO 347 355 activate activate VB 356 360 gene gene NN 361 371 expression expression NN 371 372 . . . 373 376 The the DT 377 387 mechanisms mechanism NNS 388 390 by by IN 391 396 which which WDT 397 409 beta-catenin beta-catenin NN 410 419 undergoes undergo VBZ 420 424 this this DT 425 430 shift shift NN 431 433 in in IN 434 442 location location NN 443 446 and and CC 447 459 participates participate VBZ 460 462 in in IN 463 473 activation activation NN 474 476 of of IN 477 481 gene gene NN 482 495 transcription transcription NN 496 499 are be VBP 500 507 unknown unknown JJ 507 508 . . . 509 511 We we PRP 512 523 demonstrate demonstrate VBP 524 528 here here RB 529 533 that that IN 534 546 beta-catenin beta-catenin NN 547 550 can can MD 551 553 be be VB 554 562 imported import VBN 563 567 into into IN 568 571 the the DT 572 579 nucleus nucleus NN 580 593 independently independently RB 594 596 of of IN 597 604 LEF/TCF LEF/TCF NNP 605 612 binding binding NN 612 613 , , , 614 617 and and CC 618 620 it it PRP 621 624 may may MD 625 629 also also RB 630 632 be be VB 633 641 exported export VBN 642 646 from from IN 647 653 nuclei nucleus NNS 653 654 . . . 655 657 We we PRP 658 662 have have VBP 663 673 introduced introduce VBN 674 675 a a DT 676 681 small small JJ 682 690 deletion deletion NN 691 697 within within IN 698 710 beta-catenin beta-catenin NN 711 712 ( ( ( 712 719 Delta19 delta19 NN 719 720 ) ) ) 721 725 that that WDT 726 734 disrupts disrupt VBZ 735 742 binding binding NN 743 745 to to TO 746 751 LEF-1 LEF-1 NNP 751 752 , , , 753 763 E-cadherin E-cadherin NNP 763 764 , , , 765 768 and and CC 769 772 APC APC NNP 773 776 but but CC 777 780 not not RB 781 785 axin axin NN 785 786 . . . 787 791 This this DT 792 799 Delta19 delta19 NN 800 812 beta-catenin beta-catenin NN 813 819 mutant mutant NN 820 829 localizes localize VBZ 830 832 to to TO 833 836 the the DT 837 844 nucleus nucleus NN 845 852 because because IN 853 855 it it PRP 856 859 may may MD 860 863 not not RB 864 866 be be VB 867 878 efficiently efficiently RB 879 890 sequestered sequester VBN 891 893 in in IN 894 897 the the DT 898 907 cytoplasm cytoplasm NN 907 908 . . . 909 912 The the DT 913 920 nuclear nuclear JJ 921 933 localization localization NN 934 936 of of IN 937 944 Delta19 delta19 NN 945 957 definitively definitively RB 958 970 demonstrates demonstrate VBZ 971 975 that that IN 976 979 the the DT 980 990 mechanisms mechanism NNS 991 993 by by IN 994 999 which which WDT 1000 1012 beta-catenin beta-catenin NN 1013 1022 localizes localize VBZ 1023 1025 in in IN 1026 1029 the the DT 1030 1037 nucleus nucleus NN 1038 1041 are be VBP 1042 1052 completely completely RB 1053 1064 independent independent JJ 1065 1067 of of IN 1068 1075 LEF/TCF LEF/TCF NNP 1076 1084 factors. factors. NN 1085 1097 beta-Catenin beta-catenin NN 1098 1101 and and CC 1102 1107 LEF-1 lef-1 NN 1108 1117 complexes complex NNS 1118 1121 can can MD 1122 1130 activate activate VB 1131 1139 reporter reporter NN 1140 1144 gene gene NN 1145 1155 expression expression NN 1156 1158 in in IN 1159 1160 a a DT 1161 1172 transformed transform VBN 1173 1185 T-lymphocyte T-lymphocyte NNP 1186 1190 cell cell NN 1191 1195 line line NN 1196 1197 ( ( ( 1197 1203 Jurkat Jurkat NNP 1203 1204 ) ) ) 1205 1208 but but CC 1209 1212 not not RB 1213 1215 in in IN 1216 1222 normal normal JJ 1223 1224 T t NN 1225 1236 lymphocytes lymphocyte NNS 1236 1237 , , , 1238 1242 even even RB 1243 1249 though though IN 1250 1254 both both DT 1255 1262 factors factor NNS 1263 1266 are be VBP 1267 1274 nuclear nuclear JJ 1274 1275 . . . 1276 1280 Thus thus RB 1280 1281 , , , 1282 1294 localization localization NN 1295 1297 of of IN 1298 1302 both both DT 1303 1310 factors factor NNS 1311 1313 to to TO 1314 1317 the the DT 1318 1325 nucleus nucleus NN 1326 1328 is be VBZ 1329 1332 not not RB 1333 1343 sufficient sufficient JJ 1344 1347 for for IN 1348 1358 activation activation NN 1359 1361 of of IN 1362 1366 gene gene NN 1367 1377 expression expression NN 1377 1378 . . . 1379 1385 Excess excess JJ 1386 1398 beta-catenin beta-catenin NN 1399 1402 can can MD 1403 1410 squelch squelch VB 1411 1419 reporter reporter NN 1420 1424 gene gene NN 1425 1435 activation activation NN 1436 1438 by by IN 1439 1457 LEF-1-beta-catenin LEF-1-beta-catenin NNP 1458 1467 complexes complex NNS 1468 1471 but but CC 1472 1475 not not RB 1476 1486 activation activation NN 1487 1489 by by IN 1490 1493 the the DT 1494 1507 transcription transcription NN 1508 1514 factor factor NN 1515 1519 VP16 vp16 NN 1519 1520 . . . 1521 1526 Taken take VBN 1527 1535 together together RB 1535 1536 , , , 1537 1542 these these DT 1543 1547 data datum NNS 1548 1555 suggest suggest VBP 1556 1560 that that IN 1561 1562 a a DT 1563 1568 third third JJ 1569 1578 component component NN 1579 1581 is be VBZ 1582 1591 necessary necessary JJ 1592 1595 for for IN 1596 1600 gene gene NN 1601 1611 activation activation NN 1612 1615 and and CC 1616 1620 that that IN 1621 1625 this this DT 1626 1631 third third JJ 1632 1641 component component NN 1642 1645 may may MD 1646 1650 vary vary VB 1651 1655 with with IN 1656 1660 cell cell NN 1661 1665 type type NN 1665 1666 . . .