0 11 Paradoxical paradoxical JJ 12 19 priming priming NN 20 27 effects effect NNS 28 30 of of IN 31 36 IL-10 il-10 NN 37 39 on on IN 40 48 cytokine cytokine NN 49 59 production production NN 59 60 . . . 62 67 IL-10 IL-10 NNP 68 70 is be VBZ 71 72 a a DT 73 83 well-known well-known JJ 84 101 immunosuppressive immunosuppressive JJ 102 108 and/or and/or CC 109 126 anti-inflammatory anti-inflammatory JJ 127 135 cytokine cytokine NN 135 136 . . . 137 144 However however RB 144 145 , , , 146 148 we we PRP 149 155 report report VBP 156 158 in in FW 159 164 vitro vitro FW 165 177 experimental experimental JJ 178 185 studies study NNS 186 188 in in IN 189 194 which which WDT 195 200 IL-10 il-10 NN 201 207 primed prim VBD 208 218 leukocytes leukocyte NNS 219 222 and and CC 223 226 led lead VBD 227 229 to to TO 230 232 an an DT 233 241 enhanced enhance VBN 242 252 production production NN 253 255 of of IN 256 261 tumor tumor NN 262 270 necrosis necrosis NN 271 277 factor factor NN 278 279 ( ( ( 279 282 TNF TNF NNP 282 283 ) ) ) 284 288 upon upon IN 289 296 further further JJ 297 308 stimulation stimulation NN 309 311 by by IN 312 330 lipopolysaccharide lipopolysaccharide NN 331 332 ( ( ( 332 335 LPS LPS NNP 335 336 ) ) ) 336 337 . . . 338 347 Monocytes Monocyte NNP 348 351 and and CC 352 362 peripheral peripheral JJ 363 368 blood blood NN 369 380 mononuclear mononuclear JJ 381 386 cells cell NNS 387 388 ( ( ( 388 392 PBMC PBMC NNP 392 393 ) ) ) 394 402 prepared prepare VBN 403 407 from from IN 408 413 whole whole JJ 414 419 blood blood NN 420 430 maintained maintain VBN 431 434 for for IN 435 437 20 20 CD 438 439 h h NN 440 442 at at IN 443 445 37 37 CD 446 453 degrees degree NNS 454 455 C c JJ 456 458 in in IN 459 462 the the DT 463 471 presence presence NN 472 474 of of IN 475 486 recombinant recombinant JJ 487 492 human human JJ 493 498 IL-10 il-10 NN 499 502 had have VBD 503 505 an an DT 506 514 enhanced enhance VBN 515 523 capacity capacity NN 524 526 to to TO 527 534 produce produce VB 535 538 TNF tnf NN 539 541 in in IN 542 550 response response NN 551 553 to to TO 554 557 LPS LPS NNP 557 558 . . . 559 561 In in IN 562 570 addition addition NN 571 573 to to TO 574 577 TNF TNF NNP 577 578 , , , 579 590 LPS-induced lps-induced JJ 591 595 IL-6 IL-6 NNP 596 599 and and CC 600 611 spontaneous spontaneous JJ 612 618 IL-1ra IL-1ra NNP 619 629 production production NN 630 634 were be VBD 635 639 also also RB 640 648 enhanced enhance VBN 648 649 . . . 650 654 When when WRB 655 663 isolated isolate VBN 664 668 PBMC PBMC NNP 669 673 were be VBD 674 679 first first RB 680 688 cultured culture VBN 689 692 for for IN 693 695 20 20 CD 696 697 h h NN 698 700 in in IN 701 704 the the DT 705 713 presence presence NN 714 716 of of IN 717 722 IL-10 IL-10 NNP 723 725 on on IN 726 732 Teflon Teflon NNP 733 735 to to TO 736 743 prevent prevent VB 744 753 adherence adherence NN 753 754 , , , 755 761 washed wash VBD 762 764 to to TO 765 771 remove remove VB 772 777 IL-10 il-10 NN 778 781 and and CC 782 786 then then RB 787 794 further further RB 795 803 cultured culture VBN 804 806 in in IN 807 814 plastic plastic JJ 815 821 dishes dish NNS 822 825 for for IN 826 828 an an DT 829 839 additional additional JJ 840 842 20 20 CD 843 844 h h NN 845 847 in in IN 848 851 the the DT 852 860 presence presence NN 861 863 of of IN 864 867 LPS LPS NNP 868 870 or or CC 871 879 IL-1beta IL-1beta NNP 879 880 , , , 881 883 an an DT 884 892 enhanced enhance VBN 893 900 release release NN 901 903 of of IN 904 907 TNF TNF NNP 908 911 was be VBD 912 920 observed observe VBN 920 921 . . . 922 926 This this DT 927 930 was be VBD 931 934 not not RB 935 938 the the DT 939 943 case case NN 944 948 when when WRB 949 953 PBMC PBMC NNP 954 958 were be VBD 959 971 pre-cultured pre-cultured JJ 972 974 in in IN 975 982 plastic plastic JJ 983 994 multidishes multidish NNS 995 997 in in IN 998 1001 the the DT 1002 1010 presence presence NN 1011 1013 of of IN 1014 1019 IL-10 IL-10 NNP 1019 1020 . . . 1021 1024 TNF TNF NNP 1025 1029 mRNA mRNA NNP 1030 1040 expression expression NN 1041 1048 induced induce VBN 1049 1051 by by IN 1052 1055 LPS LPS NNP 1056 1059 was be VBD 1060 1069 decreased decrease VBN 1070 1074 when when WRB 1075 1078 the the DT 1079 1092 pre-treatment pre-treatment NN 1093 1095 of of IN 1096 1100 PBMC PBMC NNP 1101 1105 with with IN 1106 1111 IL-10 IL-10 NNP 1112 1115 was be VBD 1116 1125 performed perform VBN 1126 1128 on on IN 1129 1136 plastic plastic NN 1136 1137 , , , 1138 1145 whereas whereas IN 1146 1150 this this DT 1151 1154 was be VBD 1155 1158 not not RB 1159 1162 the the DT 1163 1167 case case NN 1168 1172 when when WRB 1173 1178 cells cell NNS 1179 1183 were be VBD 1184 1196 pre-cultured pre-cultured JJ 1197 1201 with with IN 1202 1207 IL-10 IL-10 NNP 1208 1210 on on IN 1211 1217 Teflon Teflon NNP 1217 1218 . . . 1219 1230 Furthermore furthermore RB 1230 1231 , , , 1232 1240 NFkappaB NFkappaB NNP 1241 1254 translocation translocation NN 1255 1264 following follow VBG 1265 1268 LPS LPS NNP 1269 1279 activation activation NN 1280 1283 was be VBD 1284 1290 higher high JJR 1291 1296 after after IN 1297 1302 IL-10 il-10 NN 1303 1316 pre-treatment pre-treatment NN 1317 1319 on on IN 1320 1326 Teflon Teflon NNP 1327 1331 than than IN 1332 1334 on on IN 1335 1342 plastic plastic NN 1342 1343 . . . 1344 1357 Interestingly interestingly RB 1357 1358 , , , 1359 1361 an an DT 1362 1370 enhanced enhance VBN 1371 1380 frequency frequency NN 1381 1383 of of IN 1384 1388 CD16 CD16 NNP 1389 1392 and and CC 1393 1400 CD68(+) cd68(+) JJ 1401 1406 cells cell NNS 1407 1412 among among IN 1413 1416 the the DT 1417 1424 CD14(+) cd14(+) JJ 1425 1430 cells cell NNS 1431 1434 was be VBD 1435 1443 observed observe VBN 1444 1446 in in IN 1447 1450 the the DT 1451 1459 presence presence NN 1460 1462 of of IN 1463 1468 IL-10 IL-10 NNP 1468 1469 , , , 1470 1483 independently independently RB 1484 1486 of of IN 1487 1490 the the DT 1491 1502 pre-culture pre-culture JJ 1503 1513 conditions condition NNS 1514 1516 of of IN 1517 1520 the the DT 1521 1525 PBMC PBMC NNP 1525 1526 . . . 1527 1537 Altogether altogether RB 1537 1538 , , , 1539 1544 these these DT 1545 1552 results result NNS 1553 1561 indicate indicate VBP 1562 1566 that that IN 1567 1570 the the DT 1571 1584 IL-10-induced il-10-induced JJ 1585 1598 up-regulation up-regulation NN 1599 1601 of of IN 1602 1610 cytokine cytokine NN 1611 1621 production production NN 1622 1629 depends depend VBZ 1630 1632 on on IN 1633 1636 the the DT 1637 1647 prevention prevention NN 1648 1650 of of IN 1651 1659 monocyte monocyte NN 1660 1669 adherence adherence NN 1670 1672 by by IN 1673 1676 red red JJ 1677 1682 cells cell NNS 1683 1685 in in IN 1686 1689 the the DT 1690 1695 whole whole JJ 1696 1701 blood blood NN 1702 1708 assays assay NNS 1709 1711 or or CC 1712 1714 by by IN 1715 1723 cultures culture NNS 1724 1726 of of IN 1727 1731 PBMC PBMC NNP 1732 1734 on on IN 1735 1741 Teflon Teflon NNP 1741 1742 . . . 1743 1745 In in IN 1746 1754 contrast contrast NN 1754 1755 , , , 1756 1759 the the DT 1760 1769 adherence adherence NN 1770 1779 parameter parameter NN 1780 1783 has have VBZ 1784 1786 no no DT 1787 1793 effect effect NN 1794 1796 on on IN 1797 1800 the the DT 1801 1814 IL-10-induced il-10-induced JJ 1815 1825 modulation modulation NN 1826 1828 of of IN 1829 1833 some some DT 1834 1842 monocyte monocyte NN 1843 1850 surface surface NN 1851 1858 markers marker NNS 1858 1859 . . .