0 5 STAT1 stat1 NN 6 16 activation activation NN 17 23 during during IN 24 32 monocyte monocyte NN 33 35 to to TO 36 46 macrophage macrophage NN 47 57 maturation maturation NN 57 58 : : : 59 63 role role NN 64 66 of of IN 67 75 adhesion adhesion NN 76 85 molecules molecule NNS 85 86 . . . 88 93 Human human JJ 94 103 monocytes monocyte NNS 104 112 isolated isolate VBN 113 117 from from IN 118 128 peripheral peripheral JJ 129 134 blood blood NN 135 137 of of IN 138 145 healthy healthy JJ 146 152 donors donor NNS 153 157 show show VBP 158 159 a a DT 160 174 time-dependent time-dependent JJ 175 190 differentiation differentiation NN 191 195 into into IN 196 207 macrophages macrophage NNS 208 212 upon upon IN 213 215 in in FW 216 221 vitro vitro FW 222 233 cultivation cultivation NN 233 234 , , , 235 242 closely closely RB 243 252 mimicking mimic VBG 253 258 their their PRP$ 259 261 in in FW 262 266 vivo vivo FW 267 276 migration migration NN 277 280 and and CC 281 291 maturation maturation NN 292 296 into into IN 297 310 extravascular extravascular JJ 311 318 tissues tissue NNS 318 319 . . . 320 323 The the DT 324 335 mediator(s) mediator(s) NN 336 338 of of IN 339 343 this this DT 344 354 maturation maturation NN 355 362 process process NN 363 366 has have VBZ 367 370 not not RB 371 375 been be VBN 376 379 yet yet RB 380 387 defined define VBN 387 388 . . . 389 391 We we PRP 392 404 investigated investigate VBD 405 408 the the DT 409 420 involvement involvement NN 421 423 of of IN 424 430 signal signal NN 431 442 transducers transducer NNS 443 446 and and CC 447 457 activators activator NNS 458 460 of of IN 461 474 transcription transcription NN 475 476 ( ( ( 476 480 STAT STAT NNP 480 481 ) ) ) 482 489 factors factor NNS 490 492 in in IN 493 497 this this DT 498 508 phenomenon phenomenon NN 509 512 and and CC 513 521 reported report VBD 522 525 the the DT 526 534 specific specific JJ 534 535 , , , 536 550 time-dependent time-dependent JJ 550 551 , , , 552 562 activation activation NN 563 565 of of IN 566 571 STAT1 stat1 NN 572 579 protein protein NN 580 588 starting start VBG 589 591 at at IN 592 595 day day NN 596 599 0/1 0/1 CD 600 602 of of IN 603 614 cultivation cultivation NN 615 618 and and CC 619 628 maximally maximally RB 629 638 expressed express VBN 639 641 at at IN 642 645 day day NN 646 647 5 5 CD 647 648 . . . 649 654 STAT1 stat1 NN 655 663 activity activity NN 664 667 was be VBD 668 675 evident evident JJ 676 678 on on IN 679 682 the the DT 683 687 STAT stat NN 688 695 binding binding NN 696 705 sequences sequence NNS 706 707 ( ( ( 707 710 SBE SBE NNP 710 711 ) ) ) 712 719 present present JJ 720 722 in in IN 723 726 the the DT 727 736 promoters promoter NNS 737 739 of of IN 740 745 genes gene NNS 746 751 which which WDT 752 755 are be VBP 756 768 up-regulated up-regulate VBN 769 775 during during IN 776 784 monocyte monocyte NN 785 787 to to TO 788 798 macrophage macrophage NN 799 809 maturation maturation NN 810 814 such such JJ 815 817 as as IN 818 827 FcgammaRI FcgammaRI NNP 828 831 and and CC 832 838 ICAM-1 ICAM-1 NNP 838 839 , , , 840 843 and and CC 844 846 in in IN 847 850 the the DT 851 859 promoter promoter NN 860 862 of of IN 863 866 the the DT 867 880 transcription transcription NN 881 887 factor factor NN 888 891 IFN ifn NN 892 902 regulatory regulatory JJ 903 911 factor-1 factor-1 NN 911 912 . . . 913 921 Moreover moreover RB 921 922 , , , 923 926 the the DT 927 933 effect effect NN 934 936 of of IN 937 941 cell cell NN 942 950 adhesion adhesion NN 951 953 to to TO 954 965 fibronectin fibronectin NN 966 968 or or CC 969 976 laminin laminin NN 977 980 was be VBD 981 988 studied study VBN 989 991 to to TO 992 1003 investigate investigate VB 1004 1014 mechanisms mechanism NNS 1015 1023 involved involve VBN 1024 1026 in in IN 1027 1032 STAT1 stat1 NN 1033 1043 activation activation NN 1043 1044 . . . 1045 1053 Compared compare VBN 1054 1058 with with IN 1059 1068 monocytes monocyte NNS 1069 1077 adherent adherent JJ 1078 1080 on on IN 1081 1088 plastic plastic JJ 1089 1097 surfaces surface NNS 1097 1098 , , , 1099 1106 freshly freshly RB 1107 1115 isolated isolate VBN 1116 1121 cells cell NNS 1122 1129 allowed allow VBD 1130 1132 to to TO 1133 1139 adhere adhere VB 1140 1146 either either CC 1147 1149 to to TO 1150 1162 fibronectin- fibronectin- NN 1163 1165 or or CC 1166 1180 laminin-coated laminin-coated JJ 1181 1187 flasks flask NNS 1188 1197 exhibited exhibit VBD 1198 1200 an an DT 1201 1210 increased increase VBN 1211 1216 STAT1 stat1 NN 1217 1224 binding binding NN 1225 1233 activity activity NN 1234 1238 both both CC 1239 1241 in in IN 1242 1249 control control NN 1250 1253 and and CC 1254 1256 in in IN 1257 1274 IFN-gamma-treated ifn-gamma-treated JJ 1275 1280 cells cell NNS 1280 1281 . . . 1282 1285 The the DT 1286 1295 molecular molecular JJ 1296 1302 events event NNS 1303 1310 leading lead VBG 1311 1313 to to TO 1314 1322 enhanced enhance VBN 1323 1328 STAT1 stat1 NN 1329 1339 activation activation NN 1340 1343 and and CC 1344 1352 cytokine cytokine NN 1353 1367 responsiveness responsiveness NN 1368 1377 concerned concern VBD 1378 1382 both both CC 1383 1387 Y701 y701 NN 1388 1391 and and CC 1392 1396 S727 s727 NN 1397 1402 STAT1 stat1 NN 1403 1418 phosphorylation phosphorylation NN 1418 1419 . . . 1420 1429 Exogenous exogenous JJ 1430 1438 addition addition NN 1439 1441 of of IN 1442 1454 transforming transform VBG 1455 1461 growth growth NN 1462 1473 factor-beta factor-beta NN 1473 1474 , , , 1475 1480 which which WDT 1481 1487 exerts exert VBZ 1488 1490 an an DT 1491 1501 inhibitory inhibitory JJ 1502 1508 effect effect NN 1509 1511 on on IN 1512 1516 some some DT 1517 1526 monocytic monocytic JJ 1527 1542 differentiation differentiation NN 1543 1550 markers marker NNS 1550 1551 , , , 1552 1561 inhibited inhibit VBD 1562 1572 macrophage macrophage NN 1573 1583 maturation maturation NN 1583 1584 , , , 1585 1593 integrin integrin NN 1594 1604 expression expression NN 1605 1608 and and CC 1609 1614 STAT1 stat1 NN 1615 1622 binding binding NN 1623 1631 activity activity NN 1631 1632 . . . 1633 1638 Taken take VBN 1639 1647 together together RB 1648 1653 these these DT 1654 1661 results result NNS 1662 1670 indicate indicate VBP 1671 1675 that that IN 1676 1681 STAT1 stat1 NN 1682 1687 plays play VBZ 1688 1689 a a DT 1690 1697 pivotal pivotal JJ 1698 1702 role role NN 1703 1705 in in IN 1706 1709 the the DT 1710 1736 differentiation/maturation differentiation/maturation NN 1737 1744 process process NN 1745 1747 of of IN 1748 1757 monocytes monocyte NNS 1758 1760 as as IN 1761 1763 an an DT 1764 1769 early early JJ 1770 1783 transcription transcription NN 1784 1790 factor factor NN 1791 1800 initially initially RB 1801 1810 activated activate VBN 1811 1813 by by IN 1814 1823 adherence adherence NN 1824 1827 and and CC 1828 1832 then then RB 1833 1837 able able JJ 1838 1840 to to TO 1841 1849 modulate modulate VB 1850 1853 the the DT 1854 1864 expression expression NN 1865 1867 of of IN 1868 1878 functional functional JJ 1879 1884 genes gene NNS 1884 1885 , , , 1886 1890 such such JJ 1891 1893 as as IN 1894 1900 ICAM-1 ICAM-1 NNP 1901 1904 and and CC 1905 1914 FcgammaRI FcgammaRI NNP 1914 1915 . . .