0 11 Stimulation stimulation NN 12 14 of of IN 15 25 neutrophil neutrophil NN 26 39 interleukin-8 interleukin-8 NN 40 50 production production NN 51 53 by by IN 54 64 eosinophil eosinophil NN 65 72 granule granule NN 73 78 major major JJ 79 84 basic basic JJ 85 92 protein protein NN 92 93 . . . 95 97 We we PRP 98 107 evaluated evaluate VBD 108 111 the the DT 112 119 ability ability NN 120 122 of of IN 123 133 eosinophil eosinophil NN 134 141 granule granule NN 142 147 major major JJ 148 153 basic basic JJ 154 161 protein protein NN 162 163 ( ( ( 163 166 MBP MBP NNP 166 167 ) ) ) 168 170 to to TO 171 180 stimulate stimulate VB 181 192 interleukin interleukin NN 193 194 ( ( ( 194 199 IL)-8 il)-8 NN 200 210 production production NN 211 213 by by IN 214 225 neutrophils neutrophil NNS 225 226 . . . 227 230 MBP MBP NNP 231 235 over over IN 236 239 the the DT 240 253 concentration concentration NN 254 259 range range NN 260 262 of of IN 263 266 0.1 0.1 CD 267 269 to to TO 270 272 10 10 CD 273 279 microM microm NN 280 290 stimulated stimulate VBD 291 294 the the DT 295 302 release release NN 303 305 of of IN 306 308 up up IN 309 311 to to TO 312 325 approximately approximately RB 326 327 8 8 CD 328 333 ng/ml ng/ml NN 334 338 IL-8 il-8 NN 338 339 . . . 340 350 Incubation Incubation NNP 351 355 with with IN 356 357 2 2 CD 358 364 microM microM NNP 365 368 MBP MBP NNP 369 375 showed show VBD 376 380 that that IN 380 381 , , , 382 387 after after IN 388 389 a a DT 390 391 1 1 CD 392 393 h h NN 394 397 lag lag NN 397 398 , , , 399 402 the the DT 403 408 level level NN 409 411 of of IN 412 416 IL-8 il-8 NN 417 424 release release NN 425 434 increased increase VBD 435 439 with with IN 440 444 time time NN 445 448 for for IN 449 462 approximately approximately RB 463 465 10 10 CD 466 467 h h NN 467 468 . . . 469 471 At at IN 472 475 the the DT 476 477 2 2 CD 478 484 microM microm NN 485 498 concentration concentration NN 498 499 , , , 500 510 eosinophil eosinophil NN 511 519 cationic cationic JJ 520 527 protein protein NN 527 528 , , , 529 547 eosinophil-derived eosinophil-derived JJ 548 558 neurotoxin neurotoxin NN 558 559 , , , 560 563 and and CC 564 574 eosinophil eosinophil NN 575 585 peroxidase peroxidase NN 586 589 did do VBD 590 593 not not RB 594 603 stimulate stimulate VB 604 615 significant significant JJ 616 622 levels level NNS 623 625 of of IN 626 630 IL-8 il-8 NN 631 641 production production NN 641 642 . . . 643 646 MBP MBP NNP 647 657 stimulated stimulate VBD 658 664 2-fold 2-fold JJ 665 674 increases increase NNS 675 677 in in IN 678 682 IL-8 il-8 NN 683 692 messenger messenger NN 693 696 RNA RNA NNP 697 698 ( ( ( 698 702 mRNA mRNA NNP 702 703 ) ) ) 704 709 after after IN 710 711 1 1 CD 712 715 and and CC 716 717 3 3 CD 718 719 h h NN 720 722 of of IN 723 733 incubation incubation NN 733 734 , , , 735 740 which which WDT 741 745 were be VBD 746 753 blocked block VBN 754 756 by by IN 757 769 pretreatment pretreatment NN 770 774 with with IN 775 786 actinomycin actinomycin NN 787 788 D D NNP 788 789 . . . 790 797 However however RB 797 798 , , , 799 810 stimulation stimulation NN 811 815 with with IN 816 819 MBP MBP NNP 820 823 did do VBD 824 827 not not RB 828 835 produce produce VB 836 838 an an DT 839 847 increase increase NN 848 850 in in IN 851 854 the the DT 855 862 binding binding NN 863 871 activity activity NN 872 874 of of IN 875 882 nuclear nuclear JJ 883 889 factor factor NN 890 891 ( ( ( 891 901 NF)-kappaB NF)-kappaB NNP 902 904 or or CC 905 914 activator activator NN 915 924 protein-1 protein-1 NN 924 925 . . . 926 928 No no DT 929 936 NF-IL-6 nf-il-6 NN 937 944 binding binding NN 945 953 activity activity NN 954 957 was be VBD 958 966 detected detect VBN 967 969 in in IN 970 973 the the DT 974 978 same same JJ 979 986 nuclear nuclear JJ 987 995 extracts extract NNS 995 996 . . . 997 999 In in IN 1000 1008 addition addition NN 1008 1009 , , , 1010 1021 stimulation stimulation NN 1022 1026 with with IN 1027 1030 MBP MBP NNP 1031 1040 prolonged prolong VBD 1041 1044 the the DT 1045 1054 stability stability NN 1055 1057 of of IN 1058 1062 IL-8 IL-8 NNP 1063 1067 mRNA mRNA NNP 1067 1068 . . . 1069 1072 MBP MBP NNP 1073 1077 also also RB 1078 1085 induced induce VBD 1086 1095 transient transient JJ 1096 1105 increases increase NNS 1106 1108 in in IN 1109 1113 mRNA mrna NN 1114 1117 for for IN 1118 1128 macrophage macrophage NN 1129 1141 inflammatory inflammatory JJ 1142 1149 protein protein NN 1150 1151 ( ( ( 1151 1162 MIP)-1alpha MIP)-1alpha NNP 1163 1166 and and CC 1167 1176 MIP-1beta MIP-1beta NNP 1176 1177 , , , 1178 1181 but but CC 1182 1185 did do VBD 1186 1189 not not RB 1190 1199 stimulate stimulate VB 1200 1203 the the DT 1204 1211 release release NN 1212 1214 of of IN 1215 1221 either either DT 1222 1231 chemokine chemokine NN 1231 1232 . . . 1233 1238 These these DT 1239 1247 findings finding NNS 1248 1256 indicate indicate VBP 1257 1261 that that IN 1262 1265 MBP MBP NNP 1266 1268 is be VBZ 1269 1278 selective selective JJ 1279 1284 among among IN 1285 1288 the the DT 1289 1299 eosinophil eosinophil NN 1300 1307 granule granule NN 1308 1316 proteins protein NNS 1317 1319 as as IN 1320 1321 a a DT 1322 1330 stimulus stimulus NN 1331 1334 for for IN 1335 1345 neutrophil neutrophil NN 1346 1350 IL-8 il-8 NN 1351 1358 release release NN 1359 1362 and and CC 1362 1363 , , , 1364 1371 further further RB 1371 1372 , , , 1373 1377 that that IN 1378 1389 stimulation stimulation NN 1390 1392 of of IN 1393 1403 neutrophil neutrophil NN 1404 1408 IL-8 il-8 NN 1409 1416 release release NN 1417 1419 by by IN 1420 1423 MBP MBP NNP 1424 1432 involves involve VBZ 1433 1437 both both CC 1438 1453 transcriptional transcriptional JJ 1454 1457 and and CC 1458 1477 posttranscriptional posttranscriptional JJ 1478 1488 regulation regulation NN 1488 1489 . . . 1490 1492 We we PRP 1493 1502 postulate postulate VBP 1503 1507 that that IN 1508 1519 MBP-induced mbp-induced JJ 1520 1527 release release NN 1528 1530 of of IN 1531 1535 IL-8 il-8 NN 1536 1538 by by IN 1539 1550 neutrophils neutrophil NNS 1551 1554 may may MD 1555 1565 contribute contribute VB 1566 1568 to to TO 1569 1572 the the DT 1573 1588 pathophysiology pathophysiology NN 1589 1591 of of IN 1592 1597 acute acute JJ 1598 1604 asthma asthma NN 1605 1608 and and CC 1609 1614 other other JJ 1615 1627 inflammatory inflammatory JJ 1628 1632 lung lung NN 1633 1641 diseases disease NNS 1641 1642 . . .