0 8 Reactive reactive JJ 9 15 oxygen oxygen NN 16 36 intermediate-release intermediate-release NN 37 39 of of IN 40 53 fibre-exposed fibre-exposed JJ 54 63 monocytes monocyte NNS 64 73 increases increase VBZ 74 86 inflammatory inflammatory JJ 87 100 cytokine-mRNA cytokine-mrna NN 101 106 level level NN 106 107 , , , 108 115 protein protein NN 116 124 tyrosine tyrosine NN 125 131 kinase kinase NNP 132 135 and and CC 136 145 NF-kappaB NF-kappaB NNP 146 154 activity activity NN 155 157 in in IN 158 169 co-cultured co-culture VBN 170 179 bronchial bronchial JJ 180 190 epithelial epithelial JJ 191 196 cells cell NNS 197 198 ( ( ( 198 205 BEAS-2B BEAS-2B NNP 205 206 ) ) ) 206 207 . . . 209 213 Some some DT 214 223 pulmonary pulmonary JJ 224 232 diseases disease NNS 233 237 like like IN 238 248 bronchitis bronchitis NN 249 251 or or CC 252 258 asthma asthma NN 259 269 bronchiale bronchiale NN 270 273 are be VBP 274 282 mediated mediate VBN 283 285 by by IN 286 298 inflammatory inflammatory JJ 299 309 mechanisms mechanism NNS 310 312 in in IN 313 322 bronchial bronchial JJ 323 333 epithelial epithelial JJ 334 339 cells cell NNS 339 340 . . . 341 349 Alveolar alveolar JJ 350 361 macrophages macrophage NNS 362 365 are be VBP 366 373 located located JJ 374 382 directly directly RB 383 385 in in IN 386 389 the the DT 390 401 surrounding surrounding NN 402 404 of of IN 405 410 these these DT 411 416 cells cell NNS 416 417 , , , 418 420 so so IN 421 425 that that IN 426 428 we we PRP 429 436 suppose suppose VBP 437 439 an an DT 440 451 interaction interaction NN 452 459 between between IN 460 470 epithelial epithelial JJ 471 476 cells cell NNS 477 480 and and CC 481 492 macrophages macrophage NNS 493 502 regarding regard VBG 503 505 to to TO 506 509 the the DT 510 517 release release NN 518 520 of of IN 521 533 inflammatory inflammatory JJ 534 543 mediators mediator NNS 543 544 . . . 545 548 For for IN 549 558 measuring measure VBG 559 562 the the DT 563 575 contribution contribution NN 576 578 of of IN 579 590 macrophages macrophage NNS 591 593 to to TO 594 597 the the DT 598 605 release release NN 606 608 of of IN 609 621 inflammatory inflammatory JJ 622 631 mediators mediator NNS 632 634 by by IN 635 644 bronchial bronchial JJ 645 655 epithelial epithelial JJ 656 661 cells cell NNS 661 662 , , , 663 665 we we PRP 666 677 established establish VBD 678 680 an an DT 681 683 in in FW 684 689 vitro vitro FW 690 695 model model NN 696 698 of of IN 699 710 co-cultured co-culture VBN 711 716 blood blood NN 717 726 monocytes monocyte NNS 727 728 ( ( ( 728 730 BM BM NNP 730 731 ) ) ) 732 735 and and CC 736 743 BEAS-2B BEAS-2B NNP 744 749 cells cell NNS 750 752 in in IN 753 754 a a DT 755 764 transwell transwell NN 765 771 system system NN 772 773 ( ( ( 773 779 Costar Costar NNP 779 780 ) ) ) 780 781 . . . 782 784 BM BM NNP 785 789 were be VBD 790 797 exposed expose VBN 798 800 to to TO 801 811 Chrysotile Chrysotile NNP 812 813 B B NNP 814 817 and and CC 818 822 soot soot NN 823 831 particle particle NN 832 834 FR FR NNP 835 838 101 101 CD 839 841 in in IN 842 843 a a DT 844 857 concentration concentration NN 858 860 of of IN 861 864 100 100 CD 865 877 microg/10(6) microg/10(6) NN 878 883 cells cell NNS 883 884 . . . 885 890 After after IN 891 893 up up IN 894 896 to to TO 897 899 90 90 CD 900 903 min min NN 904 912 exposure exposure NN 913 917 time time NN 918 923 ELISA ELISA NNP 923 924 , , , 925 929 EMSA EMSA NNP 930 931 ( ( ( 931 946 electromobility electromobility NN 947 952 shift shift NN 953 958 assay assay NN 958 959 ) ) ) 960 963 and and CC 964 970 RT-PCR RT-PCR NNP 971 975 were be VBD 976 980 used use VBN 981 983 to to TO 984 991 measure measure VB 992 999 protein protein NN 1000 1008 tyrosine tyrosine NN 1009 1015 kinase kinase NNP 1016 1024 activity activity NN 1024 1025 , , , 1026 1033 protein protein NN 1034 1042 activity activity NN 1043 1045 of of IN 1046 1055 NF-kappaB NF-kappaB NNP 1056 1059 and and CC 1060 1068 cytokine cytokine NN 1069 1070 ( ( ( 1070 1078 IL-1beta IL-1beta NNP 1078 1079 , , , 1080 1084 IL-6 IL-6 NNP 1084 1085 , , , 1086 1095 TNF-alpha TNF-alpha NNP 1095 1096 ) ) ) 1097 1105 specific specific JJ 1106 1110 mRNA mrna NN 1111 1117 levels level NNS 1118 1120 in in IN 1121 1128 BEAS-2B BEAS-2B NNP 1129 1134 cells cell NNS 1134 1135 . . . 1136 1138 We we PRP 1139 1147 observed observe VBD 1148 1150 an an DT 1151 1159 increase increase NN 1160 1162 in in IN 1163 1170 protein protein NN 1171 1179 tyrosine tyrosine NN 1180 1186 kinase kinase NNP 1187 1195 activity activity NN 1196 1197 ( ( ( 1197 1199 up up RB 1200 1202 to to TO 1203 1206 1.8 1.8 CD 1207 1210 +/- +/- CC 1211 1219 0.5-fold 0.5-fold JJ 1219 1220 ) ) ) 1221 1224 and and CC 1225 1234 NF-kappaB NF-kappaB NNP 1235 1242 protein protein NN 1243 1251 activity activity NN 1252 1254 in in IN 1255 1262 BEAS-2B BEAS-2B NNP 1263 1268 cells cell NNS 1269 1274 after after IN 1275 1283 particle particle NN 1284 1286 or or CC 1287 1292 fibre fibre NN 1293 1301 exposure exposure NN 1302 1304 of of IN 1305 1316 co-cultured co-culture VBN 1317 1319 BM bm NN 1319 1320 . . . 1321 1332 Consecutive consecutive JJ 1333 1342 IL-1beta- il-1beta- NN 1342 1343 , , , 1344 1349 IL-6- IL-6- NNP 1350 1353 and and CC 1354 1368 TNF-alpha-mRNA TNF-alpha-mRNA NNP 1369 1373 were be VBD 1374 1382 elevated elevate VBN 1383 1384 ( ( ( 1384 1386 up up RB 1387 1389 to to TO 1390 1393 1.9 1.9 CD 1394 1397 +/- +/- CC 1398 1407 0.58-fold 0.58-fold JJ 1407 1408 ) ) ) 1408 1409 . . . 1410 1417 Protein protein NN 1418 1426 tyrosine tyrosine NN 1427 1433 kinase kinase NNP 1434 1442 activity activity NN 1442 1443 , , , 1444 1453 NF-kappaB NF-kappaB NNP 1454 1462 activity activity NN 1462 1463 , , , 1464 1467 and and CC 1468 1471 the the DT 1472 1481 synthesis synthesis NN 1482 1484 of of IN 1485 1502 cytokine-specific cytokine-specific JJ 1503 1507 mRNA mRNA NNP 1508 1512 were be VBD 1513 1522 inhibited inhibit VBN 1523 1525 by by IN 1526 1538 antioxidants antioxidant NNS 1538 1539 . . . 1540 1545 These these DT 1546 1550 data datum NNS 1551 1558 suggest suggest VBP 1559 1560 a a DT 1561 1574 ROI-dependent roi-dependent JJ 1575 1584 NF-kappaB NF-kappaB NNP 1585 1593 mediated mediate VBD 1594 1607 transcription transcription NN 1608 1610 of of IN 1611 1623 inflammatory inflammatory JJ 1624 1633 cytokines cytokine NNS 1634 1636 in in IN 1637 1646 bronchial bronchial JJ 1647 1657 epithelial epithelial JJ 1658 1663 cells cell NNS 1663 1664 . . .