0	16	Thymocyte-thymic	thymocyte-thymic	JJ
17	27	epithelial	epithelial	JJ
28	32	cell	cell	NN
33	44	interaction	interaction	NN
45	50	leads	lead	VBZ
51	53	to	to	TO
54	64	high-level	high-level	JJ
65	76	replication	replication	NN
77	79	of	of	IN
80	85	human	human	JJ
86	102	immunodeficiency	immunodeficiency	NN
103	108	virus	virus	NN
109	120	exclusively	exclusively	RB
121	123	in	in	IN
124	130	mature	mature	JJ
131	137	CD4(+)	cd4(+)	JJ
138	144	CD8(-)	cd8(-)	JJ
145	151	CD3(+)	cd3(+)	JJ
152	162	thymocytes	thymocyte	NNS
162	163	:	:	:
164	165	a	a	DT
166	174	critical	critical	JJ
175	179	role	role	NN
180	183	for	for	IN
184	189	tumor	tumor	NN
190	198	necrosis	necrosis	NN
199	205	factor	factor	NN
206	209	and	and	CC
210	223	interleukin-7	interleukin-7	NN
223	224	.	.	.

226	230	This	this	DT
231	235	work	work	NN
236	240	aims	aim	VBZ
241	243	at	at	IN
244	255	identifying	identify	VBG
256	259	the	the	DT
260	269	thymocyte	thymocyte	NN
270	283	subpopulation	subpopulation	NN
284	288	able	able	JJ
289	291	to	to	TO
292	299	support	support	VB
300	305	human	human	JJ
306	322	immunodeficiency	immunodeficiency	NN
323	328	virus	virus	NN
329	330	(	(	(
330	333	HIV	HIV	NNP
333	334	)	)	)
335	346	replication	replication	NN
347	352	under	under	IN
353	356	the	the	DT
357	367	biological	biological	JJ
368	375	stimuli	stimulus	NNS
376	378	of	of	IN
379	382	the	the	DT
383	389	thymic	thymic	JJ
390	406	microenvironment	microenvironment	NN
406	407	.	.	.

408	410	In	in	IN
411	415	this	this	DT
416	422	report	report	NN
423	425	we	we	PRP
426	437	demonstrate	demonstrate	VBP
438	442	that	that	IN
443	454	interaction	interaction	NN
455	459	with	with	IN
460	466	thymic	thymic	JJ
467	477	epithelial	epithelial	JJ
478	483	cells	cell	NNS
484	485	(	(	(
485	488	TEC	TEC	NNP
488	489	)	)	)
490	497	induces	induce	VBZ
498	499	a	a	DT
500	510	high-level	high-level	JJ
511	522	replication	replication	NN
523	525	of	of	IN
526	529	the	the	DT
530	538	T-tropic	t-tropic	JJ
539	546	primary	primary	JJ
547	554	isolate	isolate	JJ
555	568	HIV-1(B-LAIp)	hiv-1(b-laip)	NN
569	580	exclusively	exclusively	RB
581	583	in	in	IN
584	587	the	the	DT
588	594	mature	mature	JJ
595	601	CD4(+)	cd4(+)	JJ
602	608	CD8(-)	cd8(-)	JJ
609	615	CD3(+)	cd3(+)	JJ
616	626	thymocytes	thymocyte	NNS
626	627	.	.	.

628	633	Tumor	tumor	NN
634	642	necrosis	necrosis	NN
643	649	factor	factor	NN
650	651	(	(	(
651	654	TNF	TNF	NNP
654	655	)	)	)
656	659	and	and	CC
660	673	interleukin-7	interleukin-7	NN
674	675	(	(	(
675	679	IL-7	IL-7	NNP
679	680	)	)	)
680	681	,	,	,
682	690	secreted	secrete	VBN
691	697	during	during	IN
698	702	this	this	DT
703	714	interaction	interaction	NN
714	715	,	,	,
716	719	are	be	VBP
720	728	critical	critical	JJ
729	738	cytokines	cytokine	NNS
739	742	for	for	IN
743	746	HIV	HIV	NNP
747	751	long	long	JJ
752	760	terminal	terminal	JJ
761	767	repeat	repeat	NN
768	783	transactivation	transactivation	NN
784	791	through	through	IN
792	811	NF-kappaB-dependent	nf-kappab-dependent	JJ
812	822	activation	activation	NN
822	823	.	.	.

824	827	TNF	TNF	NNP
828	830	is	be	VBZ
831	834	the	the	DT
835	840	major	major	JJ
841	848	inducer	inducer	NN
849	851	of	of	IN
852	861	NF-kappaB	NF-kappaB	NNP
862	865	and	and	CC
866	878	particularly	particularly	RB
879	881	of	of	IN
882	885	the	the	DT
886	893	p50-p65	p50-p65	NN
894	901	complex	complex	NN
901	902	,	,	,
903	910	whereas	whereas	IN
911	915	IL-7	il-7	NN
916	920	acts	act	VBZ
921	923	as	as	IN
924	925	a	a	DT
926	934	cofactor	cofactor	NN
935	937	by	by	IN
938	948	sustaining	sustain	VBG
949	952	the	the	DT
953	963	expression	expression	NN
964	966	of	of	IN
967	970	the	the	DT
971	974	p75	p75	NN
975	978	TNF	tnf	NN
979	987	receptor	receptor	NN
987	988	.	.	.

989	992	The	the	DT
993	1004	requirement	requirement	NN
1005	1008	for	for	IN
1009	1012	TNF	TNF	NNP
1013	1015	is	be	VBZ
1016	1023	further	further	RBR
1024	1033	confirmed	confirm	VBN
1034	1036	by	by	IN
1037	1040	the	the	DT
1041	1052	observation	observation	NN
1053	1057	that	that	IN
1058	1061	the	the	DT
1062	1071	inability	inability	NN
1072	1074	of	of	IN
1075	1078	the	the	DT
1079	1091	intermediate	intermediate	JJ
1092	1098	CD4(+)	cd4(+)	JJ
1099	1105	CD8(-)	cd8(-)	JJ
1106	1112	CD3(-)	cd3(-)	JJ
1113	1123	thymocytes	thymocyte	NNS
1124	1126	to	to	TO
1127	1136	replicate	replicate	VB
1137	1140	the	the	DT
1141	1146	virus	virus	NN
1147	1149	is	be	VBZ
1150	1160	associated	associate	VBN
1161	1165	with	with	IN
1166	1167	a	a	DT
1168	1174	defect	defect	NN
1175	1177	in	in	IN
1178	1181	TNF	TNF	NNP
1182	1192	production	production	NN
1193	1199	during	during	IN
1200	1205	their	their	PRP$
1206	1217	interaction	interaction	NN
1218	1222	with	with	IN
1223	1226	TEC	TEC	NNP
1227	1230	and	and	CC
1231	1241	correlates	correlate	VBZ
1242	1246	with	with	IN
1247	1250	the	the	DT
1251	1258	absence	absence	NN
1259	1261	of	of	IN
1262	1269	nuclear	nuclear	JJ
1270	1279	NF-kappaB	NF-kappaB	NNP
1280	1288	activity	activity	NN
1289	1291	in	in	IN
1292	1297	these	these	DT
1298	1305	freshly	freshly	RB
1306	1314	isolated	isolate	VBN
1315	1325	thymocytes	thymocyte	NNS
1325	1326	.	.	.

1327	1335	Addition	addition	NN
1336	1338	of	of	IN
1339	1348	exogenous	exogenous	JJ
1349	1352	TNF	tnf	NN
1353	1355	to	to	TO
1356	1359	the	the	DT
1360	1372	intermediate	intermediate	JJ
1373	1382	thymocyte	thymocyte	NN
1383	1391	cultures	culture	NNS
1392	1399	induces	induce	VBZ
1400	1409	NF-kappaB	NF-kappaB	NNP
1410	1418	activity	activity	NN
1419	1422	and	and	CC
1423	1425	is	be	VBZ
1426	1436	sufficient	sufficient	JJ
1437	1439	to	to	TO
1440	1447	promote	promote	VB
1448	1451	HIV	HIV	NNP
1452	1463	replication	replication	NN
1464	1466	in	in	IN
1467	1470	the	the	DT
1471	1481	cocultures	coculture	NNS
1482	1486	with	with	IN
1487	1490	TEC	TEC	NNP
1490	1491	.	.	.

1492	1495	The	the	DT
1496	1501	other	other	JJ
1502	1507	major	major	JJ
1508	1521	subpopulation	subpopulation	NN
1522	1532	expressing	express	VBG
1533	1536	the	the	DT
1537	1540	CD4	cd4	NN
1541	1549	receptor	receptor	NN
1549	1550	,	,	,
1551	1557	namely	namely	RB
1557	1558	,	,	,
1559	1562	the	the	DT
1563	1578	double-positive	double-positive	JJ
1579	1580	(	(	(
1580	1582	DP	DP	NNP
1582	1583	)	)	)
1584	1590	CD4(+)	cd4(+)	JJ
1591	1597	CD8(+)	cd8(+)	JJ
1598	1606	CD3(+/-)	cd3(+/-)	JJ
1607	1617	thymocytes	thymocyte	NNS
1617	1618	,	,	,
1619	1626	despite	despite	IN
1627	1630	the	the	DT
1631	1636	entry	entry	NN
1637	1639	of	of	IN
1640	1643	the	the	DT
1644	1649	virus	virus	NN
1649	1650	,	,	,
1651	1653	do	do	VBP
1654	1657	not	not	RB
1658	1665	produce	produce	VB
1666	1667	a	a	DT
1668	1679	significant	significant	JJ
1680	1685	level	level	NN
1686	1688	of	of	IN
1689	1694	virus	virus	NN
1694	1695	,	,	,
1696	1706	presumably	presumably	RB
1707	1714	because	because	IN
1715	1719	they	they	PRP
1720	1723	are	be	VBP
1724	1736	unresponsive	unresponsive	JJ
1737	1739	to	to	TO
1740	1743	TNF	TNF	NNP
1744	1747	and	and	CC
1748	1752	IL-7	IL-7	NNP
1752	1753	.	.	.

1754	1762	Together	together	RB
1762	1763	,	,	,
1764	1769	these	these	DT
1770	1774	data	datum	NNS
1775	1782	suggest	suggest	VBP
1783	1787	that	that	IN
1788	1790	in	in	FW
1791	1795	vivo	vivo	FW
1795	1796	,	,	,
1797	1804	despite	despite	IN
1805	1807	an	an	DT
1808	1817	efficient	efficient	JJ
1818	1823	entry	entry	NN
1824	1826	of	of	IN
1827	1830	the	the	DT
1831	1836	virus	virus	NN
1837	1839	in	in	IN
1840	1843	all	all	PDT
1844	1847	the	the	DT
1848	1854	CD4(+)	cd4(+)	JJ
1855	1869	subpopulations	subpopulation	NNS
1869	1870	,	,	,
1871	1872	a	a	DT
1873	1877	high	high	JJ
1878	1883	viral	viral	JJ
1884	1888	load	load	NN
1889	1892	may	may	MD
1893	1895	be	be	VB
1896	1905	generated	generate	VBN
1906	1917	exclusively	exclusively	RB
1918	1924	within	within	IN
1925	1928	the	the	DT
1929	1935	mature	mature	JJ
1936	1942	CD4(+)	cd4(+)	JJ
1943	1949	CD8(-)	cd8(-)	JJ
1950	1956	CD3(+)	cd3(+)	JJ
1957	1963	subset	subset	NN
1964	1966	of	of	IN
1967	1977	thymocytes	thymocyte	NNS
1977	1978	.	.	.

1979	1986	However	however	RB
1986	1987	,	,	,
1988	1993	under	under	IN
1994	2004	conditions	condition	NNS
2005	2007	of	of	IN
2008	2020	inflammatory	inflammatory	JJ
2021	2029	response	response	NN
2030	2035	after	after	IN
2036	2045	infection	infection	NN
2045	2046	,	,	,
2047	2050	TNF	TNF	NNP
2051	2056	might	might	MD
2057	2061	also	also	RB
2062	2064	be	be	VB
2065	2072	present	present	JJ
2073	2075	in	in	IN
2076	2079	the	the	DT
2080	2092	intermediate	intermediate	JJ
2093	2102	thymocyte	thymocyte	NN
2103	2114	compartment	compartment	NN
2114	2115	,	,	,
2116	2123	leading	lead	VBG
2124	2126	to	to	TO
2127	2136	efficient	efficient	JJ
2137	2140	HIV	HIV	NNP
2141	2152	replication	replication	NN
2153	2155	in	in	IN
2156	2161	these	these	DT
2162	2167	cells	cell	NNS
2167	2168	.	.	.