0 6 Signal Signal NNP 7 19 transduction transduction NN 20 28 pathways pathway NNS 29 38 triggered trigger VBN 39 41 by by IN 42 45 the the DT 46 59 FcepsilonRIIb FcepsilonRIIb NNP 60 68 receptor receptor NN 69 70 ( ( ( 70 74 CD23 CD23 NNP 74 75 ) ) ) 76 78 in in IN 79 84 human human JJ 85 94 monocytes monocyte NNS 95 99 lead lead VBP 100 102 to to TO 103 110 nuclear nuclear JJ 111 124 factor-kappaB factor-kappab NN 125 135 activation activation NN 135 136 . . . 138 148 BACKGROUND background NN 148 149 : : : 150 158 Alveolar alveolar JJ 159 170 macrophages macrophage NNS 171 175 play play VBP 176 177 a a DT 178 181 key key JJ 182 186 role role NN 187 189 in in IN 190 193 the the DT 194 204 initiation initiation NN 205 207 of of IN 208 211 the the DT 212 224 inflammatory inflammatory JJ 225 233 reaction reaction NN 234 236 of of IN 237 245 allergic allergic JJ 246 252 asthma asthma NN 252 253 . . . 254 262 Alveolar alveolar JJ 263 274 macrophages macrophage NNS 275 278 and and CC 279 289 peripheral peripheral JJ 290 295 blood blood NN 296 305 monocytes monocyte NNS 306 309 are be VBP 310 319 activated activate VBN 320 324 when when WRB 325 337 IgE/allergen IgE/allergen NNP 338 344 immune immune JJ 345 354 complexes complex NNS 355 359 bind bind VBP 360 362 to to TO 363 366 the the DT 367 371 CD23 cd23 NN 372 380 receptor receptor NN 380 381 , , , 382 387 which which WDT 388 393 leads lead VBZ 394 396 to to TO 397 400 the the DT 401 411 production production NN 412 414 of of IN 415 427 inflammatory inflammatory JJ 428 437 cytokines cytokine NNS 437 438 . . . 439 448 OBJECTIVE objective NN 448 449 : : : 450 452 We we PRP 453 459 sought seek VBD 460 462 to to TO 463 474 investigate investigate VB 475 478 the the DT 479 488 molecular molecular JJ 489 499 mechanisms mechanism NNS 500 510 regulating regulate VBG 511 515 this this DT 516 521 early early JJ 522 534 inflammatory inflammatory JJ 535 543 response response NN 543 544 . . . 545 547 We we PRP 548 552 have have VBP 553 560 focused focus VBN 561 563 on on IN 564 567 the the DT 568 573 study study NN 574 576 of of IN 577 580 the the DT 581 587 signal signal NN 588 600 transduction transduction NN 601 609 pathways pathway NNS 610 619 triggered trigger VBN 620 622 by by IN 623 627 CD23 cd23 NN 628 630 in in IN 631 636 human human JJ 637 646 monocytes monocyte NNS 647 650 and and CC 651 654 the the DT 655 667 promonocytic promonocytic JJ 668 672 cell cell NN 673 677 line line NN 678 682 U937 u937 NN 682 683 . . . 684 691 METHODS methods NNS 691 692 : : : 693 697 CD23 CD23 NNP 698 701 was be VBD 702 714 cross-linked cross-linked JJ 715 717 in in IN 718 723 human human JJ 724 733 monocytes monocyte NNS 734 737 and and CC 738 742 U937 u937 NN 743 748 cells cell NNS 749 753 with with IN 754 757 IgE ige NN 758 764 immune immune JJ 765 774 complexes complex NNS 774 775 . . . 776 783 Surface surface NN 784 794 expression expression NN 795 797 of of IN 798 802 CD23 CD23 NNP 803 806 was be VBD 807 817 determined determine VBN 818 820 by by IN 821 825 FACS FACS NNP 826 834 analysis analysis NN 834 835 . . . 836 849 Transcription Transcription NNP 850 856 factor factor NN 857 867 activation activation NN 868 871 and and CC 872 876 gene gene NN 877 890 transcription transcription NN 891 895 were be VBD 896 903 studied study VBN 904 906 by by IN 907 916 gel-shift gel-shift JJ 917 923 assays assay NNS 924 927 and and CC 928 936 Northern northern JJ 937 941 blot blot NN 942 950 analysis analysis NN 950 951 , , , 952 964 respectively respectively RB 964 965 . . . 966 978 IkappaBalpha IkappaBalpha NNP 979 994 phosphorylation phosphorylation NN 995 998 and and CC 999 1010 degradation degradation NN 1011 1014 was be VBD 1015 1023 analyzed analyze VBN 1024 1026 by by IN 1027 1034 Western western JJ 1035 1039 blot blot NN 1039 1040 . . . 1041 1048 RESULTS results NNS 1048 1049 : : : 1050 1057 Nuclear nuclear JJ 1058 1064 factor factor NN 1065 1066 ( ( ( 1066 1076 NF)-kappaB NF)-kappaB NNP 1077 1079 is be VBZ 1080 1083 the the DT 1084 1088 main main JJ 1089 1102 transcription transcription NN 1103 1109 factor factor NN 1110 1118 involved involve VBN 1119 1121 in in IN 1122 1125 the the DT 1126 1130 gene gene NN 1131 1141 activation activation NN 1142 1146 that that WDT 1147 1154 follows follow VBZ 1155 1159 CD23 cd23 NN 1160 1173 cross-linking cross-linking NN 1174 1176 in in IN 1177 1186 monocytes monocyte NNS 1186 1187 . . . 1188 1200 CD23-induced cd23-induced JJ 1201 1210 NF-kappaB NF-kappaB NNP 1211 1213 is be VBZ 1214 1215 a a DT 1216 1227 heterodimer heterodimer NN 1228 1236 composed compose VBN 1237 1239 of of IN 1240 1247 p65/p50 p65/p50 NN 1248 1256 subunits subunit NNS 1256 1257 . . . 1258 1267 NF-kappaB NF-kappaB NNP 1268 1275 nuclear nuclear JJ 1276 1289 translocation translocation NN 1290 1292 is be VBZ 1293 1302 secondary secondary JJ 1303 1305 to to TO 1306 1309 the the DT 1310 1325 phosphorylation phosphorylation NN 1326 1329 and and CC 1330 1340 subsequent subsequent JJ 1341 1352 degradation degradation NN 1353 1355 of of IN 1356 1359 the the DT 1360 1369 NF-kappaB NF-kappaB NNP 1370 1380 inhibitory inhibitory JJ 1381 1389 molecule molecule NN 1390 1402 IkappaBalpha IkappaBalpha NNP 1402 1403 . . . 1404 1412 Tyrosine Tyrosine NNP 1413 1429 kinase-dependent kinase-dependent JJ 1429 1430 , , , 1431 1434 and and CC 1435 1438 not not RB 1439 1446 protein protein NN 1447 1453 kinase kinase NNP 1454 1465 C-dependent c-dependent JJ 1465 1466 , , , 1467 1475 pathways pathway NNS 1476 1483 mediate mediate VBP 1484 1498 CD23-triggered cd23-triggered JJ 1499 1508 NF-kappaB NF-kappaB NNP 1509 1519 activation activation NN 1520 1523 but but CC 1524 1526 do do VBP 1527 1530 not not RB 1531 1542 participate participate VB 1543 1545 in in IN 1546 1549 the the DT 1550 1556 direct direct JJ 1557 1572 phosphorylation phosphorylation NN 1573 1575 of of IN 1576 1588 IkappaBalpha IkappaBalpha NNP 1588 1589 . . . 1590 1602 IkappaBalpha IkappaBalpha NNP 1603 1614 degradation degradation NN 1615 1618 and and CC 1619 1628 NF-kappaB NF-kappaB NNP 1629 1636 nuclear nuclear JJ 1637 1650 translocation translocation NN 1651 1660 correlate correlate VBP 1661 1665 with with IN 1666 1681 transcriptional transcriptional JJ 1682 1692 activation activation NN 1693 1695 of of IN 1696 1699 the the DT 1700 1712 inflammatory inflammatory JJ 1713 1722 cytokines cytokine NNS 1723 1732 TNF-alpha TNF-alpha NNP 1733 1736 and and CC 1737 1745 IL-1beta IL-1beta NNP 1745 1746 . . . 1747 1758 CONCLUSIONS conclusions NNS 1758 1759 : : : 1760 1769 NF-kappaB NF-kappaB NNP 1770 1772 is be VBZ 1773 1776 the the DT 1777 1781 main main JJ 1782 1795 transcription transcription NN 1796 1802 factor factor NN 1803 1811 involved involve VBN 1812 1814 in in IN 1815 1818 the the DT 1819 1825 signal signal NN 1826 1838 transduction transduction NN 1839 1846 pathway pathway NN 1847 1849 of of IN 1850 1854 CD23 cd23 NN 1855 1857 in in IN 1858 1867 monocytes monocyte NNS 1867 1868 . . .