0 9 Induction Induction NNP 10 12 of of IN 13 21 Bcl-x(L) bcl-x(l) NN 22 32 expression expression NN 33 35 by by IN 36 41 human human JJ 42 48 T-cell t-cell NN 49 57 leukemia leukemia NN 58 63 virus virus NN 64 68 type type NN 69 70 1 1 CD 71 74 Tax tax NN 75 82 through through IN 83 92 NF-kappaB NF-kappaB NNP 93 95 in in IN 96 115 apoptosis-resistant apoptosis-resistant JJ 116 122 T-cell t-cell NN 123 136 transfectants transfectant NNS 137 141 with with IN 142 145 Tax Tax NNP 145 146 . . . 148 153 Human human JJ 154 160 T-cell T-cell NNP 161 169 leukemia leukemia NN 170 175 virus virus NN 176 180 type type NN 181 182 1 1 CD 183 184 ( ( ( 184 190 HTLV-1 HTLV-1 NNP 190 191 ) ) ) 192 195 Tax Tax NNP 196 198 is be VBZ 199 206 thought think VBN 207 209 to to TO 210 214 play play VB 215 216 a a DT 217 224 pivotal pivotal JJ 225 229 role role NN 230 232 in in IN 233 248 immortalization immortalization NN 249 251 of of IN 252 253 T t NN 254 259 cells cell NNS 259 260 . . . 261 263 We we PRP 264 268 have have VBP 269 277 recently recently RB 278 283 shown show VBN 284 288 that that IN 289 292 the the DT 293 303 expression expression NN 304 306 of of IN 307 310 Tax Tax NNP 311 320 protected protect VBD 321 324 the the DT 325 330 mouse mouse NN 331 337 T-cell t-cell NN 338 342 line line NN 343 349 CTLL-2 ctll-2 NN 350 357 against against IN 358 367 apoptosis apoptosis NN 368 375 induced induce VBN 376 378 by by IN 379 392 interleukin-2 interleukin-2 NN 393 394 ( ( ( 394 398 IL-2 il-2 NN 398 399 ) ) ) 400 411 deprivation deprivation NN 412 415 and and CC 416 425 converted convert VBD 426 429 its its PRP$ 430 436 growth growth NN 437 441 from from IN 442 447 being be VBG 448 452 IL-2 il-2 NN 453 462 dependent dependent JJ 463 465 to to TO 466 471 being be VBG 472 476 IL-2 il-2 NN 477 488 independent independent JJ 488 489 . . . 490 492 In in IN 493 497 this this DT 498 503 study study NN 503 504 , , , 505 507 we we PRP 508 519 demonstrate demonstrate VBP 520 524 that that IN 525 537 constitutive constitutive JJ 538 548 expression expression NN 549 551 of of IN 552 558 bcl-xl bcl-xl NN 559 562 but but CC 563 566 not not RB 567 572 bcl-2 bcl-2 NN 572 573 , , , 574 580 bcl-xs bcl-x NNS 580 581 , , , 582 585 bak bak NN 585 586 , , , 587 590 bad bad NN 590 591 , , , 592 594 or or CC 595 598 bax bax NN 599 602 was be VBD 603 613 associated associate VBN 614 618 with with IN 619 628 apoptosis apoptosis NN 629 639 resistance resistance NN 640 645 after after IN 646 650 IL-2 il-2 NN 651 662 deprivation deprivation NN 663 665 in in IN 666 672 CTLL-2 ctll-2 NN 673 678 cells cell NNS 679 683 that that WDT 684 693 expressed express VBD 694 697 Tax Tax NNP 697 698 . . . 699 721 Transient-transfection Transient-transfection NNP 722 728 assays assay NNS 729 735 showed show VBD 736 740 that that IN 741 746 bcl-x bcl-x NN 747 755 promoter promoter NN 756 759 was be VBD 760 774 transactivated transactivate VBN 775 777 by by IN 778 787 wild-type wild-type JJ 788 791 Tax tax NN 791 792 . . . 793 800 Similar similar JJ 801 808 effects effect NNS 809 813 were be VBD 814 822 observed observe VBN 823 825 in in IN 826 832 mutant mutant JJ 833 836 Tax tax NN 837 846 retaining retain VBG 847 862 transactivating transactivate VBG 863 870 ability ability NN 871 878 through through IN 879 888 NF-kappaB NF-kappaB NNP 888 889 . . . 890 898 Deletion deletion NN 899 901 or or CC 902 914 substitution substitution NN 915 917 of of IN 918 919 a a DT 920 928 putative putative JJ 929 938 NF-kappaB nf-kappab NN 939 946 binding binding NN 947 951 site site NN 952 962 identified identify VBN 963 965 in in IN 966 969 the the DT 970 975 bcl-x bcl-x NN 976 984 promoter promoter NN 985 998 significantly significantly RB 999 1008 decreased decrease VBD 1009 1020 Tax-induced tax-induced JJ 1021 1036 transactivation transactivation NN 1036 1037 . . . 1038 1042 This this DT 1043 1057 NF-kappaB-like nf-kappab-like JJ 1058 1065 element element NN 1066 1069 was be VBD 1070 1074 able able JJ 1075 1077 to to TO 1078 1082 form form VB 1083 1084 a a DT 1085 1092 complex complex NN 1093 1097 with with IN 1098 1107 NF-kappaB NF-kappaB NNP 1108 1114 family family NN 1115 1123 proteins protein NNS 1124 1126 in in FW 1127 1132 vitro vitro FW 1132 1133 . . . 1134 1145 Furthermore furthermore RB 1145 1146 , , , 1147 1158 Tax-induced tax-induced JJ 1159 1174 transactivation transactivation NN 1175 1177 of of IN 1178 1181 the the DT 1182 1187 bcl-x bcl-x NN 1188 1196 promoter promoter NN 1197 1200 was be VBD 1201 1205 also also RB 1206 1216 diminished diminish VBN 1217 1219 by by IN 1220 1223 the the DT 1224 1230 mutant mutant JJ 1231 1243 IkappaBalpha IkappaBalpha NNP 1243 1244 , , , 1245 1250 which which WDT 1251 1263 specifically specifically RB 1264 1272 inhibits inhibit VBZ 1273 1282 NF-kappaB NF-kappaB NNP 1283 1291 activity activity NN 1291 1292 . . . 1293 1296 Our our PRP$ 1297 1305 findings finding NNS 1306 1313 suggest suggest VBP 1314 1318 that that IN 1319 1331 constitutive constitutive JJ 1332 1342 expression expression NN 1343 1345 of of IN 1346 1354 Bcl-x(L) bcl-x(l) NN 1355 1362 induced induce VBN 1363 1365 by by IN 1366 1369 Tax Tax NNP 1370 1377 through through IN 1378 1381 the the DT 1382 1391 NF-kappaB NF-kappaB NNP 1392 1399 pathway pathway NN 1400 1411 contributes contribute VBZ 1412 1414 to to TO 1415 1418 the the DT 1419 1429 inhibition inhibition NN 1430 1432 of of IN 1433 1442 apoptosis apoptosis NN 1443 1445 in in IN 1446 1452 CTLL-2 ctll-2 NN 1453 1458 cells cell NNS 1459 1464 after after IN 1465 1469 IL-2 il-2 NN 1470 1481 deprivation deprivation NN 1481 1482 . . .