0 3 The the DT 4 12 promoter promoter NN 13 15 of of IN 16 19 the the DT 20 24 CD19 cd19 NN 25 29 gene gene NN 30 32 is be VBZ 33 34 a a DT 35 41 target target NN 42 45 for for IN 46 49 the the DT 50 65 B-cell-specific b-cell-specific JJ 66 79 transcription transcription NN 80 86 factor factor NN 87 91 BSAP BSAP NNP 91 92 . . . 94 97 The the DT 98 102 CD19 cd19 NN 103 110 protein protein NN 111 113 is be VBZ 114 123 expressed express VBN 124 126 on on IN 127 130 the the DT 131 138 surface surface NN 139 141 of of IN 142 145 all all DT 146 156 B-lymphoid b-lymphoid JJ 157 162 cells cell NNS 163 167 with with IN 168 171 the the DT 172 181 exception exception NN 182 184 of of IN 185 195 terminally terminally RB 196 210 differentiated differentiate VBN 211 217 plasma plasma NN 218 223 cells cell NNS 224 227 and and CC 228 231 has have VBZ 232 236 been be VBN 237 247 implicated implicate VBN 248 250 as as IN 251 252 a a DT 253 271 signal-transducing signal-transducing JJ 272 280 receptor receptor NN 281 283 in in IN 284 287 the the DT 288 295 control control NN 296 298 of of IN 299 312 proliferation proliferation NN 313 316 and and CC 317 332 differentiation differentiation NN 332 333 . . . 334 338 Here here RB 339 341 we we PRP 342 353 demonstrate demonstrate VBP 354 362 complete complete JJ 363 374 correlation correlation NN 375 382 between between IN 383 386 the the DT 387 397 expression expression NN 398 405 pattern pattern NN 406 408 of of IN 409 412 the the DT 413 417 CD19 cd19 NN 418 422 gene gene NN 423 426 and and CC 427 430 the the DT 431 446 B-cell-specific b-cell-specific JJ 447 460 transcription transcription NN 461 467 factor factor NN 468 472 BSAP BSAP NNP 473 475 in in IN 476 477 a a DT 478 483 large large JJ 484 489 panel panel NN 490 492 of of IN 493 503 B-lymphoid b-lymphoid JJ 504 508 cell cell NN 509 514 lines line NNS 514 515 . . . 516 519 The the DT 520 525 human human JJ 526 530 CD19 cd19 NN 531 535 gene gene NN 536 539 has have VBZ 540 544 been be VBN 545 551 cloned clone VBN 551 552 , , , 553 556 and and CC 557 564 several several JJ 565 577 BSAP-binding bsap-binding JJ 578 583 sites site NNS 584 588 have have VBP 589 593 been be VBN 594 600 mapped map VBN 601 603 by by IN 604 606 in in FW 607 612 vitro vitro FW 613 624 protein-DNA protein-dna JJ 625 632 binding binding NN 633 640 studies study NNS 640 641 . . . 642 644 In in IN 645 655 particular particular JJ 655 656 , , , 657 658 a a DT 659 672 high-affinity high-affinity JJ 673 685 BSAP-binding bsap-binding JJ 686 690 site site NN 691 698 instead instead RB 699 701 of of IN 702 703 a a DT 704 708 TATA TATA NNP 709 717 sequence sequence NN 718 720 is be VBZ 721 728 located locate VBN 729 731 in in IN 732 735 the the DT 736 739 -30 -30 CD 740 748 promoter promoter NN 749 755 region region NN 756 764 upstream upstream JJ 765 767 of of IN 768 769 a a DT 770 777 cluster cluster NN 778 780 of of IN 781 794 heterogeneous heterogeneous JJ 795 808 transcription transcription NN 809 814 start start NN 815 820 sites site NNS 820 821 . . . 822 830 Moreover moreover RB 830 831 , , , 832 836 this this DT 837 841 site site NN 842 844 is be VBZ 845 853 occupied occupy VBN 854 856 by by IN 857 861 BSAP BSAP NNP 862 864 in in FW 865 869 vivo vivo FW 870 872 in in IN 873 874 a a DT 875 890 CD19-expressing cd19-expressing JJ 891 897 B-cell b-cell NN 898 902 line line NN 903 906 but but CC 907 910 not not RB 911 913 in in IN 914 920 plasma plasma NN 921 923 or or CC 924 928 HeLa HeLa NNP 929 934 cells cell NNS 934 935 . . . 936 940 This this DT 941 954 high-affinity high-affinity JJ 955 959 site site NN 960 963 has have VBZ 964 968 been be VBN 969 978 conserved conserve VBN 979 981 in in IN 982 985 the the DT 986 995 promoters promoter NNS 996 998 of of IN 999 1003 both both CC 1004 1009 human human JJ 1010 1013 and and CC 1014 1019 mouse mouse NN 1020 1024 CD19 cd19 NN 1025 1030 genes gene NNS 1031 1034 and and CC 1035 1038 was be VBD 1039 1050 furthermore furthermore RB 1051 1056 shown show VBN 1057 1059 to to TO 1060 1066 confer confer VB 1067 1073 B-cell b-cell NN 1074 1085 specificity specificity NN 1086 1088 to to TO 1089 1090 a a DT 1091 1102 beta-globin beta-globin NN 1103 1111 reporter reporter NN 1112 1116 gene gene NN 1117 1119 in in IN 1120 1129 transient transient JJ 1130 1142 transfection transfection NN 1143 1154 experiments experiment NNS 1154 1155 . . . 1156 1158 In in IN 1159 1167 addition addition NN 1167 1168 , , , 1169 1173 BSAP BSAP NNP 1174 1177 was be VBD 1178 1183 found find VBN 1184 1186 to to TO 1187 1189 be be VB 1190 1193 the the DT 1194 1198 only only JJ 1199 1207 abundant abundant JJ 1208 1219 DNA-binding dna-binding JJ 1220 1228 activity activity NN 1229 1231 of of IN 1232 1238 B-cell b-cell NN 1239 1246 nuclear nuclear JJ 1247 1255 extracts extract NNS 1256 1260 that that WDT 1261 1270 interacts interact VBZ 1271 1275 with with IN 1276 1279 the the DT 1280 1284 CD19 cd19 NN 1285 1293 promoter promoter NN 1293 1294 . . . 1295 1303 Together together RB 1303 1304 , , , 1305 1309 this this DT 1310 1318 evidence evidence NN 1319 1327 strongly strongly RB 1328 1338 implicates implicate VBZ 1339 1343 BSAP BSAP NNP 1344 1346 in in IN 1347 1350 the the DT 1351 1361 regulation regulation NN 1362 1364 of of IN 1365 1368 the the DT 1369 1373 CD19 cd19 NN 1374 1378 gene gene NN 1378 1379 . . .