0 5 Redox Redox NNP 6 12 status status NN 13 15 of of IN 16 21 cells cell NNS 22 32 influences influence VBZ 33 45 constitutive constitutive JJ 46 48 or or CC 49 56 induced induce VBN 57 65 NF-kappa NF-kappa NNP 66 67 B B NNP 68 81 translocation translocation NN 82 85 and and CC 86 89 HIV HIV NNP 90 94 long long JJ 95 103 terminal terminal JJ 104 110 repeat repeat NN 111 119 activity activity NN 120 122 in in IN 123 128 human human JJ 129 130 T t NN 131 134 and and CC 135 144 monocytic monocytic JJ 145 149 cell cell NN 150 155 lines line NNS 155 156 . . . 158 160 We we PRP 161 165 have have VBP 166 172 tested test VBN 173 176 the the DT 177 187 hypothesis hypothesis NN 188 192 that that IN 193 201 cellular cellular JJ 202 212 activation activation NN 213 219 events event NNS 220 229 occurring occur VBG 230 232 in in IN 233 234 T t NN 235 246 lymphocytes lymphocyte NNS 247 250 and and CC 251 260 monocytes monocyte NNS 261 264 and and CC 265 273 mediated mediate VBN 274 281 through through IN 282 295 translocation translocation NN 296 298 of of IN 299 302 the the DT 303 316 transcription transcription NN 317 323 factor factor NN 324 332 NF-kappa NF-kappa NNP 333 334 B B NNP 335 338 are be VBP 339 348 dependent dependent JJ 349 353 upon upon IN 354 357 the the DT 358 370 constitutive constitutive JJ 371 376 redox redox NN 377 383 status status NN 384 386 of of IN 387 392 these these DT 393 398 cells cell NNS 398 399 . . . 400 402 We we PRP 403 407 used use VBD 408 416 phenolic phenolic JJ 416 417 , , , 418 431 lipid-soluble lipid-soluble JJ 431 432 , , , 433 447 chain-breaking chain-breaking JJ 448 460 antioxidants antioxidant NNS 461 462 ( ( ( 462 471 butylated butylate VBN 472 486 hydroxyanisole hydroxyanisole NN 487 488 ( ( ( 488 491 BHA BHA NNP 491 492 ) ) ) 492 493 , , , 494 513 nordihydroquairetic nordihydroquairetic JJ 514 518 acid acid NN 518 519 , , , 520 522 or or CC 523 539 alpha-tocopherol alpha-tocopherol NN 540 541 ( ( ( 541 548 vitamin vitamin NN 549 550 E e NN 550 551 ) ) ) 552 554 to to TO 555 559 show show VB 560 564 that that IN 565 572 peroxyl peroxyl NN 573 580 radical radical NN 581 591 scavenging scavenging NN 592 594 in in IN 595 607 unstimulated unstimulated JJ 608 611 and and CC 612 616 PMA- pma- NN 617 619 or or CC 620 634 TNF-stimulated tnf-stimulated JJ 635 640 cells cell NNS 641 647 blocks block VBZ 648 651 the the DT 652 661 functions function NNS 662 671 depending depend VBG 672 674 on on IN 675 683 NF-kappa NF-kappa NNP 684 685 B B NNP 686 696 activation activation NN 696 697 . . . 698 701 BHA BHA NNP 702 705 was be VBD 706 711 found find VBN 712 714 to to TO 715 723 suppress suppress VB 724 727 not not RB 728 732 only only RB 733 737 PMA- pma- NN 738 740 or or CC 741 752 TNF-induced tnf-induced JJ 752 753 , , , 754 757 but but CC 758 762 also also RB 763 775 constitutive constitutive JJ 775 776 , , , 777 789 HIV-enhancer hiv-enhancer NN 790 798 activity activity NN 799 810 concomitant concomitant JJ 811 813 to to TO 814 816 an an DT 817 827 inhibition inhibition NN 828 830 of of IN 831 839 NF-kappa NF-kappa NNP 840 841 B B NNP 842 849 binding binding NN 850 858 activity activity NN 859 861 in in IN 862 866 both both CC 867 881 lymphoblastoid lymphoblastoid JJ 882 883 T t NN 884 885 ( ( ( 885 891 J.Jhan J.Jhan NNP 891 892 ) ) ) 893 896 and and CC 897 906 monocytic monocytic JJ 907 908 ( ( ( 908 912 U937 u937 NN 912 913 ) ) ) 914 918 cell cell NN 919 924 lines line NNS 924 925 . . . 926 930 This this DT 931 934 was be VBD 935 939 also also RB 940 944 true true JJ 945 948 for for IN 949 952 KBF KBF NNP 953 954 ( ( ( 954 957 p50 p50 NN 958 967 homodimer homodimer NN 967 968 ) ) ) 969 976 binding binding NN 977 985 activity activity NN 986 988 in in IN 989 993 U937 u937 NN 994 999 cells cell NNS 999 1000 . . . 1001 1010 Secretion Secretion NNP 1011 1013 of of IN 1014 1017 TNF TNF NNP 1017 1018 , , , 1019 1022 the the DT 1023 1030 product product NN 1031 1033 of of IN 1034 1041 another another DT 1042 1050 NF-kappa NF-kappa NNP 1051 1062 B-dependent b-dependent JJ 1063 1067 gene gene NN 1067 1068 , , , 1069 1072 was be VBD 1073 1082 abolished abolish VBN 1083 1085 by by IN 1086 1089 BHA BHA NNP 1090 1092 in in IN 1093 1107 PMA-stimulated pma-stimulated JJ 1108 1112 U937 u937 NN 1113 1118 cells cell NNS 1118 1119 . . . 1120 1123 The the DT 1124 1138 anti-oxidative anti-oxidative JJ 1139 1145 effect effect NN 1146 1148 of of IN 1149 1152 BHA BHA NNP 1153 1156 was be VBD 1157 1168 accompanied accompany VBN 1169 1171 by by IN 1172 1174 an an DT 1175 1183 increase increase NN 1184 1186 in in IN 1187 1192 thiol thiol NN 1192 1193 , , , 1194 1197 but but CC 1198 1201 not not RB 1202 1213 glutathione glutathione NN 1213 1214 , , , 1215 1222 content content NN 1223 1225 in in IN 1226 1236 stimulated stimulate VBN 1237 1240 and and CC 1241 1253 unstimulated unstimulated JJ 1254 1255 T t NN 1256 1260 cell cell NN 1260 1261 , , , 1262 1269 whereas whereas IN 1270 1273 TNF TNF NNP 1274 1285 stimulation stimulation NN 1286 1292 itself itself PRP 1293 1299 barely barely RB 1300 1308 modified modify VBD 1309 1312 the the DT 1313 1321 cellular cellular JJ 1322 1327 thiol thiol NN 1328 1333 level level NN 1333 1334 . . . 1335 1344 Oxidative oxidative JJ 1345 1351 stress stress NN 1352 1360 obtained obtain VBN 1361 1363 by by IN 1364 1367 the the DT 1368 1376 addition addition NN 1377 1379 of of IN 1380 1384 H2O2 h2o2 NN 1385 1387 to to TO 1388 1391 the the DT 1392 1399 culture culture NN 1400 1406 medium medium NN 1407 1409 of of IN 1410 1416 J.Jhan J.Jhan NNP 1417 1419 or or CC 1420 1424 U937 u937 NN 1425 1430 cells cell NNS 1431 1436 could could MD 1437 1440 not not RB 1441 1443 by by IN 1444 1450 itself itself PRP 1451 1457 induce induce VBP 1458 1466 NF-kappa NF-kappa NNP 1467 1468 B B NNP 1469 1479 activation activation NN 1479 1480 . . . 1481 1486 These these DT 1487 1499 observations observation NNS 1500 1507 suggest suggest VBP 1508 1512 that that IN 1513 1516 TNF TNF NNP 1517 1520 and and CC 1521 1524 PMA PMA NNP 1525 1527 do do VBP 1528 1531 not not RB 1532 1536 lead lead VB 1537 1539 to to TO 1540 1548 NF-kappa NF-kappa NNP 1549 1550 B B NNP 1551 1561 activation activation NN 1562 1569 through through IN 1570 1579 induction induction NN 1580 1582 of of IN 1583 1590 changes change NNS 1591 1593 in in IN 1594 1597 the the DT 1598 1602 cell cell NN 1603 1608 redox redox NN 1609 1615 status status NN 1615 1616 . . . 1617 1623 Rather rather RB 1623 1624 , , , 1625 1628 TNF TNF NNP 1629 1632 and and CC 1633 1636 PMA PMA NNP 1637 1640 can can MD 1641 1646 exert exert VB 1647 1652 their their PRP$ 1653 1659 effect effect NN 1660 1664 only only RB 1665 1667 if if IN 1668 1673 cells cell NNS 1674 1677 are be VBP 1678 1680 in in IN 1681 1683 an an DT 1684 1695 appropriate appropriate JJ 1696 1701 redox redox NN 1702 1708 status status NN 1708 1709 , , , 1710 1717 because because IN 1718 1723 prior prior JJ 1724 1736 modification modification NN 1737 1743 toward toward IN 1744 1753 reduction reduction NN 1754 1758 with with IN 1759 1762 BHA BHA NNP 1763 1772 treatment treatment NN 1773 1781 prevents prevent VBZ 1782 1786 this this DT 1787 1797 activation activation NN 1797 1798 . . . 1799 1801 It it PRP 1802 1809 appears appear VBZ 1810 1814 that that IN 1815 1816 a a DT 1817 1822 basal basal JJ 1823 1828 redox redox NN 1829 1840 equilibrium equilibrium NN 1841 1848 tending tend VBG 1849 1855 toward toward IN 1856 1865 oxidation oxidation NN 1866 1868 is be VBZ 1869 1870 a a DT 1871 1883 prerequisite prerequisite NN 1884 1887 for for IN 1888 1892 full full JJ 1893 1903 activation activation NN 1904 1906 of of IN 1907 1919 transduction transduction NN 1920 1928 pathways pathway NNS 1929 1939 regulating regulate VBG 1940 1943 the the DT 1944 1952 activity activity NN 1953 1955 of of IN 1956 1964 NF-kappa NF-kappa NNP 1965 1976 B-dependent b-dependent JJ 1977 1982 genes gene NNS 1982 1983 . . .