0 6 Simple simple JJ 7 17 derivation derivation NN 18 20 of of IN 21 36 TFIID-dependent tfiid-dependent JJ 37 40 RNA RNA NNP 41 51 polymerase polymerase NN 52 54 II ii CD 55 68 transcription transcription NN 69 76 systems system NNS 77 81 from from IN 82 101 Schizosaccharomyces schizosaccharomyces FW 102 107 pombe pombe FW 108 111 and and CC 112 117 other other JJ 118 127 organisms organism NNS 127 128 , , , 129 132 and and CC 133 140 factors factor NNS 141 149 required require VBN 150 153 for for IN 154 169 transcriptional transcriptional JJ 170 180 activation activation NN 180 181 . . . 183 193 Resolution Resolution NNP 194 196 of of IN 197 202 whole whole JJ 203 207 cell cell NN 208 215 extract extract NN 216 223 through through IN 224 227 two two CD 228 243 chromatographic chromatographic JJ 244 249 steps step NNS 250 256 yields yield VBZ 257 258 a a DT 259 265 single single JJ 266 273 protein protein NN 274 282 fraction fraction NN 283 292 requiring require VBG 293 297 only only RB 298 301 the the DT 302 310 addition addition NN 311 313 of of IN 314 319 TFIID TFIID NNP 320 323 for for IN 324 327 the the DT 328 338 initiation initiation NN 339 341 of of IN 342 355 transcription transcription NN 356 358 at at IN 359 362 RNA RNA NNP 363 373 polymerase polymerase NN 374 376 II ii CD 377 386 promoters promoter NNS 386 387 . . . 388 392 This this DT 393 401 approach approach NN 402 408 allows allow VBZ 409 412 the the DT 413 423 convenient convenient JJ 424 434 generation generation NN 435 437 of of IN 438 441 RNA RNA NNP 442 452 polymerase polymerase NN 453 455 II ii CD 456 469 transcription transcription NN 470 477 systems system NNS 478 482 from from IN 483 496 Saccharomyces Saccharomyces NNP 497 507 cerevisiae cerevisiae NNP 507 508 , , , 509 514 human human JJ 515 526 lymphocytes lymphocyte NNS 526 527 , , , 528 531 and and CC 532 551 Schizosaccharomyces schizosaccharomyces FW 552 557 pombe pombe FW 557 558 . . . 559 565 TFIIDs tfiid NNS 566 570 from from IN 571 574 all all DT 575 580 three three CD 581 590 organisms organism NNS 591 594 are be VBP 595 610 interchangeable interchangeable JJ 611 616 among among IN 617 620 all all DT 621 626 three three CD 627 634 systems system NNS 634 635 . . . 636 639 The the DT 640 642 S. s. FW 643 653 cerevisiae cerevisiae FW 654 657 and and CC 658 662 Sch. sch. FW 663 668 pombe pombe FW 669 676 systems system NNS 677 684 support support VBP 685 692 effects effect NNS 693 695 of of IN 696 702 acidic acidic JJ 703 712 activator activator NN 713 721 proteins protein NNS 721 722 , , , 723 731 provided provide VBN 732 733 a a DT 734 741 further further JJ 742 749 protein protein NN 750 758 fraction fraction NN 759 763 from from IN 764 766 S. s. FW 767 777 cerevisiae cerevisiae FW 778 780 is be VBZ 781 789 supplied supply VBN 789 790 . . . 791 795 This this DT 796 803 further further JJ 804 812 fraction fraction NN 813 815 is be VBZ 816 824 distinct distinct JJ 825 829 from from IN 830 833 the the DT 834 842 mediator mediator NN 843 845 of of IN 846 861 transcriptional transcriptional JJ 862 872 activation activation NN 873 882 described describe VBN 883 893 previously previously RB 894 897 and and CC 898 908 represents represent VBZ 909 910 a a DT 911 917 second second JJ 918 927 component component NN 928 930 in in IN 931 939 addition addition NN 940 942 to to TO 943 950 general general JJ 951 961 initiation initiation NN 962 969 factors factor NNS 970 974 that that WDT 975 978 may may MD 979 989 facilitate facilitate VB 990 991 a a DT 992 1000 response response NN 1001 1003 to to TO 1004 1010 acidic acidic JJ 1011 1021 activators activator NNS 1021 1022 . . .