0 1 A a DT 2 7 novel novel JJ 8 25 mitogen-inducible mitogen-inducible JJ 26 30 gene gene NN 31 38 product product NN 39 46 related relate VBN 47 49 to to TO 50 67 p50/p105-NF-kappa p50/p105-nf-kappa NN 68 69 B B NNP 70 82 participates participate VBZ 83 85 in in IN 86 101 transactivation transactivation NN 102 109 through through IN 110 111 a a DT 112 117 kappa kappa NN 118 119 B B NNP 120 124 site site NN 124 125 . . . 127 128 A a DT 129 140 Rel-related rel-related JJ 140 141 , , , 142 159 mitogen-inducible mitogen-inducible JJ 159 160 , , , 161 166 kappa kappa NN 167 176 B-binding b-binding JJ 177 184 protein protein NN 185 188 has have VBZ 189 193 been be VBN 194 200 cloned clone VBN 201 203 as as IN 204 206 an an DT 207 222 immediate-early immediate-early JJ 223 233 activation activation NN 234 238 gene gene NN 239 241 of of IN 242 247 human human JJ 248 258 peripheral peripheral JJ 259 264 blood blood NN 265 266 T t NN 267 272 cells cell NNS 272 273 . . . 274 277 The the DT 278 282 cDNA cDNA NNP 283 286 has have VBZ 287 289 an an DT 290 294 open open JJ 295 302 reading reading NN 303 308 frame frame NN 309 311 of of IN 312 315 900 900 CD 316 321 amino amino NN 322 327 acids acid NNS 328 335 capable capable JJ 336 338 of of IN 339 347 encoding encode VBG 348 349 a a DT 350 356 97-kDa 97-kda JJ 357 364 protein protein NN 364 365 . . . 366 370 This this DT 371 378 protein protein NN 379 381 is be VBZ 382 386 most most RBS 387 394 similar similar JJ 395 397 to to TO 398 401 the the DT 402 409 105-kDa 105-kda JJ 410 419 precursor precursor NN 420 431 polypeptide polypeptide NN 432 434 of of IN 435 447 p50-NF-kappa p50-nf-kappa NN 448 450 B. B. NNP 451 455 Like like IN 456 459 the the DT 460 467 105-kDa 105-kda JJ 468 477 precursor precursor NN 477 478 , , , 479 481 it it PRP 482 490 contains contain VBZ 491 493 an an DT 494 508 amino-terminal amino-terminal JJ 509 520 Rel-related rel-related JJ 521 527 domain domain NN 528 530 of of IN 531 536 about about IN 537 540 300 300 CD 541 546 amino amino NN 547 552 acids acid NNS 553 556 and and CC 557 558 a a DT 559 575 carboxy-terminal carboxy-terminal JJ 576 582 domain domain NN 583 593 containing contain VBG 594 597 six six CD 598 602 full full JJ 603 607 cell cell NN 608 613 cycle cycle NN 614 616 or or CC 617 624 ankyrin ankyrin NN 625 632 repeats repeat NNS 632 633 . . . 634 636 In in IN 637 653 vitro-translated vitro-translated JJ 654 662 proteins protein NNS 662 663 , , , 664 673 truncated truncate VBN 674 684 downstream downstream RB 685 687 of of IN 688 691 the the DT 692 695 Rel Rel NNP 696 702 domain domain NN 703 706 and and CC 707 716 excluding exclude VBG 717 720 the the DT 721 728 repeats repeat NNS 728 729 , , , 730 734 bind bind VBP 735 740 kappa kappa NN 741 742 B B NNP 743 748 sites site NNS 748 749 . . . 750 752 We we PRP 753 758 refer refer VBP 759 761 to to TO 762 765 the the DT 766 771 kappa kappa NN 772 781 B-binding b-binding NN 781 782 , , , 783 792 truncated truncate VBN 793 800 protein protein NN 801 803 as as IN 804 808 p50B p50B NNP 809 811 by by IN 812 819 analogy analogy NN 820 824 with with IN 825 837 p50-NF-kappa p50-nf-kappa NN 838 839 B B NNP 840 843 and and CC 844 846 to to TO 847 850 the the DT 851 862 full-length full-length JJ 863 870 protein protein NN 871 873 as as IN 874 878 p97. p97. NNP 879 883 p50B p50B NNP 884 886 is be VBZ 887 891 able able JJ 892 894 to to TO 895 899 form form VB 900 911 heteromeric heteromeric JJ 912 917 kappa kappa NN 918 927 B-binding b-binding JJ 928 937 complexes complex NNS 938 942 with with IN 943 947 RelB RelB NNP 947 948 , , , 949 951 as as RB 952 956 well well RB 957 959 as as IN 960 964 with with IN 965 968 p65 p65 NN 969 972 and and CC 973 976 p50 p50 NN 976 977 , , , 978 981 the the DT 982 985 two two CD 986 994 subunits subunit NNS 995 997 of of IN 998 1006 NF-kappa NF-kappa NNP 1007 1009 B. B. NNP 1010 1032 Transient-transfection Transient-transfection NNP 1033 1044 experiments experiment NNS 1045 1047 in in IN 1048 1057 embryonal embryonal JJ 1058 1067 carcinoma carcinoma NN 1068 1073 cells cell NNS 1074 1085 demonstrate demonstrate VBP 1086 1087 a a DT 1088 1098 functional functional JJ 1099 1110 cooperation cooperation NN 1111 1118 between between IN 1119 1123 p50B p50B NNP 1124 1127 and and CC 1128 1132 RelB RelB NNP 1133 1135 or or CC 1136 1139 p65 p65 NN 1140 1142 in in IN 1143 1158 transactivation transactivation NN 1159 1161 of of IN 1162 1163 a a DT 1164 1172 reporter reporter NN 1173 1180 plasmid plasmid NN 1181 1190 dependent dependent JJ 1191 1193 on on IN 1194 1195 a a DT 1196 1201 kappa kappa NN 1202 1203 B B NNP 1204 1208 site site NN 1208 1209 . . . 1210 1213 The the DT 1214 1218 data datum NNS 1219 1224 imply imply VBP 1225 1228 the the DT 1229 1238 existence existence NN 1239 1241 of of IN 1242 1243 a a DT 1244 1251 complex complex JJ 1252 1258 family family NN 1259 1261 of of IN 1262 1270 NF-kappa NF-kappa NNP 1271 1277 B-like b-like JJ 1278 1291 transcription transcription NN 1292 1299 factors factor NNS 1299 1300 . . .