0 3 The the DT 4 5 B B NNP 6 19 cell-specific cell-specific JJ 20 27 nuclear nuclear JJ 28 34 factor factor NN 35 40 OTF-2 otf-2 NN 41 51 positively positively RB 52 61 regulates regulate VBZ 62 75 transcription transcription NN 76 78 of of IN 79 82 the the DT 83 88 human human JJ 89 94 class class NN 95 97 II ii CD 98 113 transplantation transplantation NN 114 118 gene gene NN 118 119 , , , 120 123 DRA DRA NNP 123 124 . . . 126 129 The the DT 130 138 promoter promoter NN 139 141 of of IN 142 145 the the DT 146 151 major major JJ 152 170 histocompatibility histocompatibility NN 171 176 class class NN 177 179 II ii CD 180 184 gene gene NN 185 188 DRA DRA NNP 189 197 contains contain VBZ 198 200 an an DT 201 208 octamer octamer NN 209 216 element element NN 217 218 ( ( ( 218 226 ATTTGCAT ATTTGCAT NNP 226 227 ) ) ) 228 232 that that WDT 233 235 is be VBZ 236 244 required require VBN 245 248 for for IN 249 258 efficient efficient JJ 259 262 DRA DRA NNP 263 273 expression expression NN 274 276 in in IN 277 278 B B NNP 279 284 cells cell NNS 284 285 . . . 286 293 Several several JJ 294 305 DNA-binding dna-binding JJ 306 314 proteins protein NNS 315 318 are be VBP 319 324 known know VBN 325 327 to to TO 328 332 bind bind VB 333 337 this this DT 338 346 sequence sequence NN 346 347 . . . 348 351 The the DT 352 356 best good JJS 357 370 characterized characterize VBN 371 374 are be VBP 375 378 the the DT 379 380 B B NNP 381 394 cell-specific cell-specific JJ 395 400 OTF-2 otf-2 NN 401 404 and and CC 405 408 the the DT 409 419 ubiquitous ubiquitous JJ 420 425 OTF-1 otf-1 NN 425 426 . . . 427 431 This this DT 432 438 report report NN 439 447 directly directly RB 448 460 demonstrates demonstrate VBZ 461 465 that that IN 466 471 OTF-2 otf-2 NN 472 475 but but CC 476 479 not not RB 480 485 OTF-1 otf-1 NN 486 495 regulates regulate VBZ 496 499 the the DT 500 503 DRA DRA NNP 504 508 gene gene NN 508 509 . . . 510 512 In in FW 513 518 vitro vitro FW 519 532 transcription transcription NN 533 541 analysis analysis NN 542 547 using use VBG 548 555 protein protein NN 556 565 fractions fraction NNS 566 574 enriched enrich VBN 575 578 for for IN 579 582 the the DT 583 598 octamer-binding octamer-binding JJ 599 606 protein protein NN 607 612 OTF-2 otf-2 NN 613 624 demonstrate demonstrate VBP 625 626 a a DT 627 635 positive positive JJ 636 646 functional functional JJ 647 651 role role NN 652 655 for for IN 656 661 OTF-2 otf-2 NN 662 664 in in IN 665 668 DRA DRA NNP 669 673 gene gene NN 674 687 transcription transcription NN 687 688 . . . 689 691 In in IN 692 700 contrast contrast NN 700 701 , , , 702 716 OTF-1-enriched otf-1-enriched JJ 717 724 protein protein NN 725 734 fractions fraction NNS 735 738 did do VBD 739 742 not not RB 743 749 affect affect VB 750 753 DRA DRA NNP 754 758 gene gene NN 759 772 transcription transcription NN 773 781 although although IN 782 784 it it PRP 785 797 functionally functionally RB 798 806 enhanced enhance VBD 807 810 the the DT 811 824 transcription transcription NN 825 827 of of IN 828 835 another another DT 836 840 gene gene NN 840 841 . . . 842 853 Recombinant recombinant JJ 854 859 OTF-2 otf-2 NN 860 867 protein protein NN 868 876 produced produce VBN 877 879 by by IN 880 882 in in FW 883 888 vitro vitro FW 889 914 transcription/translation transcription/translation NN 915 920 could could MD 921 925 also also RB 926 933 enhance enhance VB 934 937 DRA DRA NNP 938 942 gene gene NN 943 956 transcription transcription NN 957 959 in in FW 960 965 vitro vitro FW 965 966 . . . 967 969 In in FW 970 974 vivo vivo FW 975 984 transient transient JJ 985 997 transfection transfection NN 998 1005 studies study NNS 1006 1015 utilizing utilize VBG 1016 1018 an an DT 1019 1024 OTF-2 otf-2 NN 1025 1035 expression expression NN 1036 1042 vector vector NN 1043 1051 resulted result VBD 1052 1054 in in IN 1055 1062 similar similar JJ 1063 1071 findings finding NNS 1071 1072 : : : 1073 1077 that that IN 1078 1083 OTF-2 otf-2 NN 1084 1091 protein protein NN 1092 1100 enhanced enhance VBD 1101 1104 DRA DRA NNP 1105 1109 gene gene NN 1110 1123 transcription transcription NN 1123 1124 , , , 1125 1128 and and CC 1129 1133 that that IN 1134 1138 this this DT 1139 1145 effect effect NN 1146 1154 requires require VBZ 1155 1157 an an DT 1158 1164 intact intact JJ 1165 1172 octamer octamer NN 1173 1180 element element NN 1180 1181 . . . 1182 1190 Together together RB 1191 1196 these these DT 1197 1204 results result NNS 1205 1215 constitute constitute VBP 1216 1219 the the DT 1220 1225 first first JJ 1226 1232 direct direct JJ 1233 1241 evidence evidence NN 1242 1244 of of IN 1245 1246 a a DT 1247 1255 positive positive JJ 1256 1260 role role NN 1261 1264 for for IN 1265 1268 the the DT 1269 1286 lymphoid-specific lymphoid-specific JJ 1287 1302 octamer-binding octamer-binding JJ 1303 1309 factor factor NN 1310 1312 in in IN 1313 1316 DRA DRA NNP 1317 1321 gene gene NN 1322 1335 transcription transcription NN 1335 1336 . . .