0 18 Heterodimerization Heterodimerization NNP 19 22 and and CC 23 38 transcriptional transcriptional JJ 39 49 activation activation NN 50 52 in in FW 53 58 vitro vitro FW 59 61 by by IN 62 70 NF-kappa NF-kappa NNP 71 72 B B NNP 73 81 proteins protein NNS 81 82 . . . 84 87 The the DT 88 96 NF-kappa NF-kappa NNP 97 98 B B NNP 99 105 family family NN 106 108 of of IN 109 122 transcription transcription NN 123 131 proteins protein NNS 132 142 represents represent VBZ 143 151 multiple multiple JJ 152 155 DNA dna NN 156 163 binding binding NN 163 164 , , , 165 168 rel rel NN 169 176 related relate VBN 177 189 polypeptides polypeptide NNS 190 194 that that WDT 195 205 contribute contribute VBP 206 208 to to TO 209 219 regulation regulation NN 220 222 of of IN 223 228 genes gene NNS 229 237 involved involve VBN 238 240 in in IN 241 247 immune immune JJ 248 262 responsiveness responsiveness NN 263 266 and and CC 267 279 inflammation inflammation NN 279 280 , , , 281 283 as as RB 284 288 well well RB 289 291 as as IN 292 302 activation activation NN 303 305 of of IN 306 309 the the DT 310 313 HIV HIV NNP 314 318 long long JJ 319 327 terminal terminal JJ 328 334 repeat repeat NN 334 335 . . . 336 338 In in IN 339 343 this this DT 344 349 study study NN 350 358 multiple multiple JJ 359 367 NF-kappa NF-kappa NNP 368 369 B B NNP 370 377 related related JJ 378 390 polypeptides polypeptide NNS 391 398 ranging range VBG 399 403 from from IN 404 406 85 85 CD 407 409 to to TO 410 412 45 45 CD 413 416 kDa kda NN 417 421 were be VBD 422 430 examined examine VBN 431 434 for for IN 435 440 their their PRP$ 441 449 capacity capacity NN 450 452 to to TO 453 461 interact interact VB 462 466 with with IN 467 470 the the DT 471 476 PRDII PRDII NNP 477 487 regulatory regulatory JJ 488 495 element element NN 496 498 of of IN 499 509 interferon interferon NN 510 514 beta beta NN 515 518 and and CC 519 523 were be VBD 524 529 shown show VBN 530 532 to to TO 533 540 possess possess VB 541 549 distinct distinct JJ 550 559 intrinsic intrinsic JJ 560 563 DNA dna NN 564 571 binding binding NN 572 582 affinities affinity NNS 583 586 for for IN 587 591 this this DT 592 600 NF-kappa NF-kappa NNP 601 602 B B NNP 603 607 site site NN 608 611 and and CC 612 616 form form VB 617 625 multiple multiple JJ 626 629 DNA dna NN 630 637 binding binding NN 638 643 homo- homo- JJ 644 647 and and CC 648 659 heterodimer heterodimer NN 660 669 complexes complex NNS 670 672 in in IN 673 688 co-renaturation co-renaturation NN 689 700 experiments experiment NNS 700 701 . . . 702 713 Furthermore furthermore RB 713 714 , , , 715 720 using use VBG 721 724 DNA DNA NNP 725 734 templates template NNS 735 745 containing contain VBG 746 749 two two CD 750 756 copies copy NNS 757 759 of of IN 760 763 the the DT 764 769 PRDII PRDII NNP 770 776 domain domain NN 777 783 linked link VBN 784 786 to to TO 787 790 the the DT 791 797 rabbit rabbit NN 798 802 beta beta NN 803 809 globin globin NN 810 814 gene gene NN 814 815 , , , 816 819 the the DT 820 828 purified purify VBN 829 841 polypeptides polypeptide NNS 842 854 specifically specifically RB 855 865 stimulated stimulate VBD 866 874 NF-kappa NF-kappa NNP 875 876 B B NNP 877 886 dependent dependent JJ 887 900 transcription transcription NN 901 903 in in IN 904 906 an an DT 907 909 in in FW 910 915 vitro vitro FW 916 930 reconstitution reconstitution NN 931 936 assay assay NN 937 939 as as IN 940 952 heterodimers heterodimer NNS 953 956 but but CC 957 960 not not RB 961 963 as as IN 964 967 p50 p50 NN 968 978 homodimers homodimer NNS 978 979 . . . 980 985 These these DT 986 997 experiments experiment NNS 998 1007 emphasize emphasize VBP 1008 1011 the the DT 1012 1016 role role NN 1017 1019 of of IN 1020 1028 NF-kappa NF-kappa NNP 1029 1030 B B NNP 1031 1043 dimerization dimerization NN 1044 1046 as as IN 1047 1048 a a DT 1049 1057 distinct distinct JJ 1058 1063 level level NN 1064 1066 of of IN 1067 1082 transcriptional transcriptional JJ 1083 1090 control control NN 1091 1095 that that WDT 1096 1099 may may MD 1100 1106 permit permit VB 1107 1117 functional functional JJ 1118 1133 diversification diversification NN 1134 1136 of of IN 1137 1138 a a DT 1139 1146 limited limited JJ 1147 1153 number number NN 1154 1156 of of IN 1157 1167 regulatory regulatory JJ 1168 1176 proteins protein NNS 1176 1177 . . .