0 13 Transcription Transcription NNP 14 20 factor factor NN 21 25 AP-2 ap-2 NN 26 35 activates activate VBZ 36 40 gene gene NN 41 51 expression expression NN 52 54 of of IN 55 61 HTLV-I HTLV-I NNP 61 62 . . . 64 67 The the DT 68 74 HTLV-I HTLV-I NNP 75 78 LTR LTR NNP 79 87 contains contain VBZ 88 93 three three CD 94 103 conserved conserve VBN 104 114 regulatory regulatory JJ 115 123 elements element NNS 124 129 known know VBN 130 132 as as IN 133 135 21 21 CD 136 140 base base NN 141 145 pair pair NN 146 153 repeats repeat NNS 154 159 which which WDT 160 163 are be VBP 164 172 required require VBN 173 176 for for IN 177 188 stimulation stimulation NN 189 191 of of IN 192 196 gene gene NN 197 207 expression expression NN 208 210 by by IN 211 214 the the DT 215 229 transactivator transactivator NN 230 237 protein protein NN 238 241 tax tax NN 241 242 . . . 243 254 Mutagenesis Mutagenesis NNP 255 264 indicates indicate VBZ 265 269 that that IN 270 273 the the DT 274 276 21 21 CD 277 279 bp bp NN 280 287 repeats repeat NNS 288 291 can can MD 292 294 be be VB 295 305 subdivided subdivide VBN 306 310 into into IN 311 316 three three CD 317 323 motifs motif NNS 323 324 , , , 325 326 A A NNP 326 327 , , , 328 329 B B NNP 330 333 and and CC 334 335 C C NNP 335 336 , , , 337 341 each each DT 342 344 of of IN 345 350 which which WDT 351 361 influences influence VBZ 362 365 the the DT 366 371 level level NN 372 374 of of IN 375 378 tax tax NN 379 389 activation activation NN 389 390 . . . 391 394 The the DT 395 396 A a NN 397 401 site site NN 402 404 in in IN 405 408 the the DT 409 411 21 21 CD 412 414 bp bp NN 415 421 repeat repeat NN 422 425 has have VBZ 426 432 strong strong JJ 433 441 homology homology NN 442 446 with with IN 447 457 previously previously RB 458 467 described describe VBN 468 475 binding bind VBG 476 481 sites site NNS 482 485 for for IN 486 489 the the DT 490 503 transcription transcription NN 504 510 factor factor NN 511 515 AP-2 ap-2 NN 515 516 . . . 517 519 We we PRP 520 532 demonstrated demonstrate VBD 533 537 that that IN 538 542 AP-2 AP-2 NNP 543 547 mRNA mRNA NNP 548 551 was be VBD 552 559 present present JJ 560 562 in in IN 563 576 T-lymphocytes T-lymphocyte NNP 577 580 and and CC 581 585 that that IN 586 594 cellular cellular JJ 595 602 factors factor NNS 603 607 from from IN 608 612 both both CC 613 628 non-transformed non-transformed JJ 629 632 and and CC 633 644 transformed transform VBN 645 658 T-lymphocytes t-lymphocyte NNS 659 671 specifically specifically RB 672 677 bound bind VBD 678 680 to to TO 681 684 the the DT 685 694 consensus consensus NN 695 700 motif motif NN 701 704 for for IN 705 709 AP-2 ap-2 NN 710 712 in in IN 713 717 each each DT 718 720 21 21 CD 721 723 bp bp NN 723 724 . . . 725 727 To to TO 728 737 determine determine VB 738 741 the the DT 742 746 role role NN 747 749 of of IN 750 754 AP-2 ap-2 NN 755 757 in in IN 758 761 the the DT 762 772 regulation regulation NN 773 775 of of IN 776 779 the the DT 780 786 HTLV-I HTLV-I NNP 787 790 LTR LTR NNP 791 795 gene gene NN 796 806 expression expression NN 806 807 , , , 808 810 we we PRP 811 815 used use VBD 816 818 an an DT 819 823 AP-2 ap-2 NN 824 828 cDNA cdna NN 829 831 in in IN 832 835 DNA DNA NNP 836 843 binding binding NN 844 847 and and CC 848 857 transient transient JJ 858 868 expression expression NN 869 875 assays assay NNS 875 876 . . . 877 880 Gel Gel NNP 881 892 retardation retardation NN 893 896 and and CC 897 908 methylation methylation NN 909 921 interference interference NN 922 929 studies study NNS 930 938 revealed reveal VBD 939 943 that that IN 944 955 bacterially bacterially RB 956 964 produced produce VBN 965 969 AP-2 ap-2 NN 970 975 bound bind VBD 976 988 specifically specifically RB 989 992 and and CC 993 997 with with IN 998 1002 high high JJ 1003 1011 affinity affinity NN 1012 1014 to to TO 1015 1018 all all DT 1019 1024 three three CD 1025 1027 21 21 CD 1028 1030 bp bp NN 1031 1038 repeats repeat NNS 1038 1039 , , , 1040 1043 and and CC 1044 1048 that that IN 1049 1051 it it PRP 1052 1060 required require VBD 1061 1064 the the DT 1065 1069 core core NN 1070 1078 sequence sequence NN 1079 1083 AGGC AGGC NNP 1084 1087 for for IN 1088 1096 specific specific JJ 1097 1104 binding binding NN 1104 1105 . . . 1106 1113 Binding binding NN 1114 1116 of of IN 1117 1121 AP-2 ap-2 NN 1122 1131 prevented prevent VBD 1132 1135 the the DT 1136 1146 subsequent subsequent JJ 1147 1154 binding binding NN 1155 1157 of of IN 1158 1165 members member NNS 1166 1168 of of IN 1169 1172 the the DT 1173 1181 CREB/ATF CREB/ATF NNP 1182 1188 family family NN 1189 1191 to to TO 1192 1194 an an DT 1195 1203 adjacent adjacent JJ 1204 1214 regulatory regulatory JJ 1215 1220 motif motif NN 1221 1223 in in IN 1224 1227 the the DT 1228 1230 21 21 CD 1231 1233 bp bp NN 1234 1240 repeat repeat NN 1240 1241 . . . 1242 1254 Transfection transfection NN 1255 1257 of of IN 1258 1260 an an DT 1261 1265 AP-2 ap-2 NN 1266 1276 expression expression NN 1277 1286 construct construct NN 1287 1291 into into IN 1292 1305 T-lymphocytes t-lymphocyte NNS 1306 1315 activated activate VBD 1316 1320 gene gene NN 1321 1331 expression expression NN 1332 1336 from from IN 1337 1340 the the DT 1341 1347 HTLV-I HTLV-I NNP 1348 1351 LTR LTR NNP 1351 1352 . . . 1353 1355 At at IN 1356 1361 least least JJS 1362 1365 two two CD 1366 1368 21 21 CD 1369 1371 bp bp NN 1372 1379 repeats repeat NNS 1380 1384 were be VBD 1385 1393 required require VBN 1394 1397 for for IN 1398 1402 high high JJ 1403 1409 levels level NNS 1410 1412 of of IN 1413 1417 AP-2 ap-2 NN 1418 1428 activation activation NN 1429 1432 and and CC 1433 1444 mutagenesis mutagenesis NN 1445 1447 of of IN 1448 1451 the the DT 1452 1456 AP-2 ap-2 NN 1457 1466 consensus consensus NN 1467 1474 binding binding NN 1475 1484 sequences sequence NNS 1485 1487 in in IN 1488 1491 the the DT 1492 1494 21 21 CD 1495 1497 bp bp NN 1498 1505 repeats repeat NNS 1506 1515 eliminate eliminate VBP 1516 1520 this this DT 1521 1531 activation activation NN 1531 1532 . . . 1533 1534 ( ( ( 1534 1542 ABSTRACT abstract NN 1543 1552 TRUNCATED truncated VBN 1553 1555 AT at IN 1556 1559 250 250 CD 1560 1565 WORDS words NNS 1565 1566 ) ) )