0	8	NF-kappa	NF-kappa	NNP
9	10	B	B	NNP
11	21	activation	activation	NN
22	24	by	by	IN
25	30	tumor	tumor	NN
31	39	necrosis	necrosis	NN
40	46	factor	factor	NN
47	52	alpha	alpha	NN
53	55	in	in	IN
56	59	the	the	DT
60	66	Jurkat	Jurkat	NNP
67	68	T	t	NN
69	73	cell	cell	NN
74	78	line	line	NN
79	81	is	be	VBZ
82	93	independent	independent	JJ
94	96	of	of	IN
97	104	protein	protein	NN
105	111	kinase	kinase	NNP
112	113	A	A	NNP
113	114	,	,	,
115	122	protein	protein	NN
123	129	kinase	kinase	NNP
130	131	C	C	NNP
131	132	,	,	,
133	136	and	and	CC
137	153	Ca(2+)-regulated	ca(2+)-regulated	JJ
154	161	kinases	kinase	NNS
161	162	.	.	.

164	172	NF-kappa	NF-kappa	NNP
173	174	B	B	NNP
175	177	is	be	VBZ
178	179	a	a	DT
180	191	DNA-binding	dna-binding	JJ
192	202	regulatory	regulatory	JJ
203	209	factor	factor	NN
210	214	able	able	JJ
215	217	to	to	TO
218	225	control	control	VB
226	239	transcription	transcription	NN
240	242	of	of	IN
243	244	a	a	DT
245	251	number	number	NN
252	254	of	of	IN
255	260	genes	gene	NNS
260	261	,	,	,
262	271	including	include	VBG
272	277	human	human	JJ
278	294	immunodeficiency	immunodeficiency	NN
295	300	virus	virus	NN
301	302	(	(	(
302	305	HIV	HIV	NNP
305	306	)	)	)
307	312	genes	gene	NNS
312	313	.	.	.

314	316	In	in	IN
317	318	T	t	NN
319	324	cells	cell	NNS
324	325	,	,	,
326	334	NF-kappa	NF-kappa	NNP
335	336	B	B	NNP
337	339	is	be	VBZ
340	349	activated	activate	VBN
350	354	upon	upon	IN
355	363	cellular	cellular	JJ
364	373	treatment	treatment	NN
374	376	by	by	IN
377	384	phorbol	phorbol	NN
385	391	esters	ester	NNS
392	395	and	and	CC
396	399	the	the	DT
400	408	cytokine	cytokine	NN
409	414	tumor	tumor	NN
415	423	necrosis	necrosis	NN
424	430	factor	factor	NN
431	436	alpha	alpha	NN
437	438	(	(	(
438	441	TNF	TNF	NNP
442	447	alpha	alpha	NN
447	448	)	)	)
448	449	.	.	.

450	452	In	in	IN
453	456	the	the	DT
457	464	present	present	JJ
465	469	work	work	NN
469	470	,	,	,
471	473	we	we	PRP
474	486	investigated	investigate	VBD
487	490	the	the	DT
491	500	molecular	molecular	JJ
501	507	events	event	NNS
508	515	leading	lead	VBG
516	518	to	to	TO
519	527	NF-kappa	NF-kappa	NNP
528	529	B	B	NNP
530	540	activation	activation	NN
541	543	by	by	IN
544	547	TNF	TNF	NNP
548	553	alpha	alpha	NN
554	556	in	in	IN
557	558	a	a	DT
559	564	human	human	JJ
565	566	T	t	NN
567	571	cell	cell	NN
572	576	line	line	NN
577	578	(	(	(
578	584	Jurkat	Jurkat	NNP
584	585	)	)	)
586	589	and	and	CC
590	593	its	its	PRP$
594	602	subclone	subclone	NN
603	607	JCT6	jct6	NN
607	608	,	,	,
609	614	which	which	WDT
615	623	presents	present	VBZ
624	625	a	a	DT
626	636	deficiency	deficiency	NN
637	639	in	in	IN
640	643	the	the	DT
644	647	PKA	PKA	NNP
648	660	transduction	transduction	NN
661	668	pathway	pathway	NN
668	669	.	.	.

670	672	We	we	PRP
673	678	found	find	VBD
679	683	that	that	IN
684	686	in	in	IN
687	691	both	both	DT
692	696	cell	cell	NN
697	702	lines	line	NNS
702	703	,	,	,
704	708	both	both	CC
709	716	phorbol	phorbol	NN
717	722	ester	ester	NN
723	726	and	and	CC
727	730	TNF	tnf	NN
731	736	alpha	alpha	NN
737	741	were	be	VBD
742	746	able	able	JJ
747	749	to	to	TO
750	758	activate	activate	VB
759	767	NF-kappa	NF-kappa	NNP
768	770	B.	B.	NNP
771	778	Phorbol	Phorbol	NNP
779	789	activation	activation	NN
790	793	was	be	VBD
794	804	positively	positively	RB
805	814	modulated	modulate	VBN
815	817	by	by	IN
818	822	Ca2+	ca2+	NN
823	829	influx	influx	NN
830	835	while	while	IN
836	839	TNF	TNF	NNP
840	845	alpha	alpha	NN
846	856	activation	activation	NN
857	860	was	be	VBD
861	864	not	not	RB
864	865	.	.	.

866	877	Furthermore	furthermore	RB
877	878	,	,	,
879	884	while	while	IN
885	888	PMA	PMA	NNP
889	899	activation	activation	NN
900	903	was	be	VBD
904	913	inhibited	inhibit	VBN
914	916	by	by	IN
917	920	the	the	DT
921	924	PKC	PKC	NNP
925	934	inhibitor	inhibitor	NN
935	947	staurosporin	staurosporin	NN
947	948	,	,	,
949	952	the	the	DT
953	956	TNF	TNF	NNP
957	962	alpha	alpha	NN
963	969	effect	effect	NN
970	973	was	be	VBD
974	983	unchanged	unchanged	JJ
983	984	.	.	.

985	988	TNF	TNF	NNP
989	994	alpha	alpha	NN
995	998	did	do	VBD
999	1002	not	not	RB
1003	1011	activate	activate	VB
1012	1016	cAMP	camp	NN
1017	1027	production	production	NN
1028	1031	and	and	CC
1032	1035	its	its	PRP$
1036	1042	signal	signal	NN
1043	1046	was	be	VBD
1047	1050	not	not	RB
1051	1060	modulated	modulate	VBN
1061	1063	by	by	IN
1064	1068	cAMP	camp	NN
1069	1079	activators	activator	NNS
1079	1080	.	.	.

1081	1089	Moreover	moreover	RB
1089	1090	,	,	,
1091	1095	cAMP	camp	NN
1096	1106	activators	activator	NNS
1107	1110	did	do	VBD
1111	1114	not	not	RB
1115	1123	activate	activate	VB
1124	1132	NF-kappa	NF-kappa	NNP
1133	1134	B	B	NNP
1135	1137	in	in	IN
1138	1144	Jurkat	Jurkat	NNP
1145	1150	cells	cell	NNS
1150	1151	.	.	.

1152	1156	Thus	thus	RB
1156	1157	,	,	,
1158	1161	TNF	TNF	NNP
1162	1175	alpha-induced	alpha-induced	JJ
1176	1184	NF-kappa	NF-kappa	NNP
1185	1186	B	B	NNP
1187	1197	activation	activation	NN
1198	1201	was	be	VBD
1202	1207	found	find	VBN
1208	1210	to	to	TO
1211	1213	be	be	VB
1214	1222	mediated	mediate	VBN
1223	1225	by	by	IN
1226	1230	none	none	NN
1231	1233	of	of	IN
1234	1237	the	the	DT
1238	1243	major	major	JJ
1244	1260	signal-mediating	signal-mediating	JJ
1261	1268	kinases	kinase	NNS
1269	1273	such	such	JJ
1274	1276	as	as	IN
1277	1284	protein	protein	NN
1285	1291	kinase	kinase	NNP
1292	1293	C	C	NNP
1294	1295	(	(	(
1295	1298	PKC	PKC	NNP
1298	1299	)	)	)
1299	1300	,	,	,
1301	1308	protein	protein	NN
1309	1315	kinase	kinase	NNP
1316	1317	A	A	NNP
1317	1318	,	,	,
1319	1321	or	or	CC
1322	1338	Ca(2+)-regulated	ca(2+)-regulated	JJ
1339	1346	kinases	kinase	NNS
1346	1347	.	.	.

1348	1359	Furthermore	furthermore	RB
1359	1360	,	,	,
1361	1363	we	we	PRP
1364	1369	found	find	VBD
1370	1374	that	that	IN
1375	1386	cytoplasmic	cytoplasmic	JJ
1387	1400	acidification	acidification	NN
1401	1412	facilitated	facilitate	VBD
1413	1421	NF-kappa	NF-kappa	NNP
1422	1423	B	B	NNP
1424	1434	activation	activation	NN
1435	1437	by	by	IN
1438	1442	both	both	CC
1443	1446	TNF	TNF	NNP
1447	1452	alpha	alpha	NN
1453	1456	and	and	CC
1457	1460	PKC	PKC	NNP
1460	1461	,	,	,
1462	1464	by	by	IN
1465	1466	a	a	DT
1467	1476	mechanism	mechanism	NN
1477	1481	that	that	WDT
1482	1491	increases	increase	VBZ
1492	1500	NF-kappa	NF-kappa	NNP
1501	1504	B/I	B/I	NNP
1505	1510	kappa	kappa	NN
1511	1512	B	B	NNP
1513	1525	dissociation	dissociation	NN
1526	1533	without	without	IN
1534	1543	affecting	affect	VBG
1544	1547	the	the	DT
1548	1556	NF-kappa	NF-kappa	NNP
1557	1558	B	B	NNP
1559	1572	translocation	translocation	NN
1573	1577	step	step	NN
1577	1578	.	.	.