0 2 An an DT 3 12 erythroid erythroid JJ 13 21 specific specific JJ 22 30 enhancer enhancer NN 31 39 upstream upstream JJ 40 42 to to TO 43 46 the the DT 47 51 gene gene NN 52 60 encoding encode VBG 61 64 the the DT 65 74 cell-type cell-type JJ 75 83 specific specific JJ 84 97 transcription transcription NN 98 104 factor factor NN 105 111 GATA-1 gata-1 NN 111 112 . . . 114 117 The the DT 118 131 transcription transcription NN 132 138 factor factor NN 139 145 GATA-1 gata-1 NN 146 148 is be VBZ 149 158 expressed express VBN 159 161 in in IN 162 163 a a DT 164 170 subset subset NN 171 173 of of IN 174 185 hemopoietic hemopoietic JJ 186 191 cells cell NNS 191 192 , , , 193 198 where where WRB 199 201 it it PRP 202 210 mediates mediate VBZ 211 214 the the DT 215 224 cell-type cell-type JJ 225 233 specific specific JJ 234 244 expression expression NN 245 247 of of IN 248 255 several several JJ 256 261 genes gene NNS 261 262 . . . 263 265 We we PRP 266 270 have have VBP 271 277 cloned clone VBN 278 281 the the DT 282 287 mouse mouse NN 288 291 and and CC 292 297 human human JJ 298 304 GATA-1 gata-1 NN 305 310 genes gene NNS 310 311 . . . 312 313 A a DT 314 320 region region NN 321 329 upstream upstream JJ 330 332 to to TO 333 336 the the DT 337 342 first first JJ 343 347 exon exon NN 347 348 , , , 349 352 and and CC 353 359 highly highly RB 360 369 conserved conserve VBN 370 377 between between IN 378 383 mouse mouse NN 384 387 and and CC 388 391 man man NN 391 392 , , , 393 397 acts act VBZ 398 400 as as IN 401 403 an an DT 404 413 erythroid erythroid JJ 414 422 specific specific JJ 423 431 enhancer enhancer NN 432 434 in in IN 435 444 transient transient JJ 445 451 assays assay NNS 451 452 , , , 453 455 if if IN 456 462 linked link VBN 463 465 to to TO 466 469 the the DT 470 476 GATA-1 gata-1 NN 477 479 or or CC 480 482 to to TO 483 486 the the DT 487 491 SV40 sv40 NN 492 500 promoter promoter NN 500 501 . . . 502 505 The the DT 506 514 activity activity NN 515 517 of of IN 518 521 the the DT 522 530 enhancer enhancer NN 531 533 is be VBZ 534 540 almost almost RB 541 551 completely completely RB 552 561 dependent dependent JJ 562 564 on on IN 565 568 the the DT 569 578 integrity integrity NN 579 581 of of IN 582 583 a a DT 584 591 dimeric dimeric JJ 592 598 GATA-1 gata-1 NN 599 606 binding binding NN 607 611 site site NN 611 612 . . .