0 5 Clone Clone NNP 6 9 pAT pat NN 10 13 133 133 CD 14 24 identifies identify VBZ 25 26 a a DT 27 31 gene gene NN 32 36 that that WDT 37 44 encodes encode VBZ 45 52 another another DT 53 58 human human JJ 59 65 member member NN 66 68 of of IN 69 70 a a DT 71 76 class class NN 77 79 of of IN 80 86 growth growth NN 87 101 factor-induced factor-induced JJ 102 107 genes gene NNS 108 112 with with IN 113 119 almost almost RB 120 129 identical identical JJ 130 141 zinc-finger zinc-finger NN 142 149 domains domain NNS 149 150 . . . 152 154 We we PRP 155 161 report report VBP 162 165 the the DT 166 175 structure structure NN 176 179 and and CC 180 190 regulation regulation NN 191 193 of of IN 194 195 a a DT 196 200 gene gene NN 201 212 represented represent VBN 213 215 by by IN 216 221 clone clone NN 222 225 pAT pat NN 226 229 133 133 CD 229 230 , , , 231 236 which which WDT 237 239 is be VBZ 240 247 induced induce VBN 248 252 upon upon IN 253 263 transition transition NN 264 268 from from IN 269 270 a a DT 271 278 resting resting JJ 279 284 state state NN 285 286 ( ( ( 286 288 G0 G0 NNP 288 289 ) ) ) 290 297 through through IN 298 301 the the DT 302 307 early early JJ 308 313 phase phase NN 314 316 of of IN 317 320 the the DT 321 325 cell cell NN 326 331 cycle cycle NN 332 333 ( ( ( 333 335 G1 G1 NNP 335 336 ) ) ) 336 337 . . . 338 341 The the DT 342 345 pAT pat NN 346 349 133 133 CD 350 354 gene gene NN 355 357 is be VBZ 358 369 immediately immediately RB 370 377 induced induce VBN 377 378 , , , 379 383 with with IN 384 392 FOS-like fos-like JJ 393 401 kinetics kinetic NNS 401 402 , , , 403 405 in in IN 406 411 human human JJ 412 413 T t NN 414 419 cells cell NNS 420 423 and and CC 424 426 in in IN 427 438 fibroblasts fibroblast NNS 438 439 . . . 440 447 Primary primary JJ 448 457 structure structure NN 458 466 analysis analysis NN 467 473 showed show VBD 474 478 that that IN 479 482 the the DT 483 490 encoded encode VBN 491 498 protein protein NN 499 507 contains contain VBZ 508 513 three three CD 514 520 tandem tandem JJ 521 532 zinc-finger zinc-finger NN 533 542 sequences sequence NNS 543 545 of of IN 546 549 the the DT 550 554 type type NN 555 570 Cys2-Xaa12-His2 cys2-xaa12-his2 NN 570 571 . . . 572 576 This this DT 577 588 zinc-finger zinc-finger NN 589 595 region region NN 595 596 , , , 597 602 which which WDT 603 605 is be VBZ 606 613 thought think VBN 614 616 to to TO 617 621 bind bind VB 622 625 DNA dna NN 626 628 in in IN 629 630 a a DT 631 648 sequence-specific sequence-specific JJ 649 655 manner manner NN 655 656 , , , 657 659 is be VBZ 660 667 similar similar JJ 668 669 ( ( ( 669 676 greater great JJR 677 681 than than IN 682 684 80 80 CD 684 685 % % NN 686 688 on on IN 689 692 the the DT 693 698 amino amino JJ 699 703 acid acid NN 704 709 level level NN 709 710 ) ) ) 711 713 to to TO 714 717 two two CD 718 728 previously previously RB 729 738 described describe VBN 739 752 transcription transcription NN 753 760 factors factor NNS 761 764 pAT pat NN 765 773 225/EGR1 225/egr1 NN 774 777 and and CC 778 781 pAT pat NN 782 790 591/EGR2 591/egr2 NN 790 791 . . . 792 798 Except except IN 799 802 for for IN 803 806 the the DT 807 816 conserved conserve VBN 817 828 zinc-finger zinc-finger NN 829 836 domains domain NNS 836 837 , , , 838 841 the the DT 842 847 amino amino JJ 848 852 acid acid NN 853 862 sequences sequence NNS 863 865 of of IN 866 869 the the DT 870 875 three three CD 876 884 proteins protein NNS 885 888 are be VBP 889 897 distinct distinct JJ 897 898 . . . 899 903 This this DT 904 914 structural structural JJ 915 925 similarity similarity NN 926 934 suggests suggest VBZ 935 939 that that IN 940 943 the the DT 944 947 pAT pat NN 948 951 133 133 CD 952 956 gene gene NN 957 964 encodes encode VBZ 965 966 a a DT 967 980 transcription transcription NN 981 987 factor factor NN 988 992 with with IN 993 994 a a DT 995 1003 specific specific JJ 1004 1014 biological biological JJ 1015 1023 function function NN 1023 1024 . . . 1025 1034 Comparing compare VBG 1035 1038 the the DT 1039 1049 regulation regulation NN 1050 1052 of of IN 1053 1058 these these DT 1059 1066 related relate VBN 1067 1087 zinc-finger-encoding zinc-finger-encoding JJ 1088 1093 genes gene NNS 1094 1100 showed show VBD 1101 1111 coordinate coordinate JJ 1112 1121 induction induction NN 1122 1126 upon upon IN 1127 1136 mitogenic mitogenic JJ 1137 1148 stimulation stimulation NN 1149 1151 of of IN 1152 1159 resting rest VBG 1160 1161 T t NN 1162 1173 lymphocytes lymphocyte NNS 1174 1177 and and CC 1178 1180 of of IN 1181 1188 resting rest VBG 1189 1200 fibroblasts fibroblast NNS 1200 1201 . . . 1202 1209 However however RB 1209 1210 , , , 1211 1215 upon upon IN 1216 1226 transition transition NN 1227 1231 from from IN 1232 1233 a a DT 1234 1247 proliferating proliferate VBG 1248 1249 ( ( ( 1249 1251 G1 g1 NN 1251 1252 ) ) ) 1253 1255 to to TO 1256 1257 a a DT 1258 1265 resting resting JJ 1266 1271 state state NN 1272 1274 of of IN 1275 1278 the the DT 1279 1283 cell cell NN 1284 1289 cycle cycle NN 1290 1293 the the DT 1294 1299 three three CD 1300 1305 genes gene NNS 1306 1310 were be VBD 1311 1322 differently differently RB 1323 1332 regulated regulate VBN 1332 1333 . . . 1334 1336 In in IN 1337 1342 human human JJ 1343 1354 histiocytic histiocytic JJ 1355 1359 U937 u937 NN 1360 1365 cells cell NNS 1366 1370 mRNA mrna NN 1371 1373 of of IN 1374 1379 clone clone NN 1380 1383 pAT pat NN 1384 1387 133 133 CD 1388 1391 was be VBD 1392 1406 constitutively constitutively RB 1407 1416 expressed express VBN 1416 1417 , , , 1418 1425 whereas whereas IN 1426 1430 mRNA mrna NN 1431 1433 of of IN 1434 1437 pAT pat NN 1438 1446 225/EGR1 225/egr1 NN 1447 1450 was be VBD 1451 1458 induced induce VBN 1459 1463 upon upon IN 1464 1473 induction induction NN 1474 1476 of of IN 1477 1485 terminal terminal JJ 1486 1501 differentiation differentiation NN 1501 1502 . . . 1503 1505 In in IN 1506 1514 contrast contrast NN 1515 1519 mRNA mRNA NNP 1520 1532 representing represent VBG 1533 1536 pAT pat NN 1537 1545 591/EGR2 591/egr2 NN 1546 1549 was be VBD 1550 1553 not not RB 1554 1563 expressed express VBN 1564 1566 in in IN 1567 1572 these these DT 1573 1578 cells cell NNS 1578 1579 . . . 1580 1584 This this DT 1585 1595 difference difference NN 1596 1598 in in IN 1599 1603 gene gene NN 1604 1614 regulation regulation NN 1615 1623 suggests suggest VBZ 1624 1632 distinct distinct JJ 1633 1643 biological biological JJ 1644 1649 roles role NNS 1650 1652 in in IN 1653 1656 the the DT 1657 1664 control control NN 1665 1667 of of IN 1668 1672 cell cell NN 1673 1686 proliferation proliferation NN 1687 1690 for for IN 1691 1694 the the DT 1695 1705 respective respective JJ 1706 1714 proteins protein NNS 1714 1715 . . .