0 11 Stimulation stimulation NN 12 14 of of IN 15 25 interferon interferon NN 26 30 beta beta NN 31 35 gene gene NN 36 49 transcription transcription NN 50 52 in in FW 53 58 vitro vitro FW 59 61 by by IN 62 70 purified purify VBN 71 79 NF-kappa NF-kappa NNP 80 81 B B NNP 82 85 and and CC 86 87 a a DT 88 93 novel novel JJ 94 96 TH th NN 97 104 protein protein NN 104 105 . . . 107 110 The the DT 111 116 human human JJ 117 127 interferon interferon NN 128 132 beta beta NN 133 134 ( ( ( 134 142 IFN-beta IFN-beta NNP 142 143 ) ) ) 144 154 regulatory regulatory JJ 155 162 element element NN 163 171 consists consist VBZ 172 174 of of IN 175 183 multiple multiple JJ 184 192 enhanson enhanson NN 193 200 domains domain NNS 201 206 which which WDT 207 210 are be VBP 211 218 targets target NNS 219 222 for for IN 223 236 transcription transcription NN 237 244 factors factor NNS 245 253 involved involve VBN 254 256 in in IN 257 266 inducible inducible JJ 267 277 expression expression NN 278 280 of of IN 281 284 the the DT 285 293 promoter promoter NN 293 294 . . . 295 297 To to TO 298 305 further further RB 306 318 characterize characterize VB 319 322 the the DT 323 334 protein-DNA protein-dna JJ 335 347 interactions interaction NNS 348 357 mediating mediate VBG 358 366 IFN-beta IFN-beta NNP 367 376 induction induction NN 376 377 , , , 378 386 positive positive JJ 387 397 regulatory regulatory JJ 398 404 domain domain NN 405 406 ( ( ( 406 409 PRD PRD NNP 409 410 ) ) ) 411 413 II ii CD 414 421 binding bind VBG 422 430 proteins protein NNS 431 435 were be VBD 436 444 purified purify VBN 445 449 from from IN 450 457 phorbol phorbol NN 458 463 ester ester NN 464 471 induced induce VBD 472 478 Jurkat Jurkat NNP 479 486 T-cells T-cell NNP 487 490 and and CC 491 495 from from IN 496 499 IFN IFN NNP 500 506 primed prim VBN 506 507 , , , 508 548 cycloheximide/polyinosinic-polycytidylic cycloheximide/polyinosinic-polycytidylic JJ 549 553 acid acid NN 554 561 treated treat VBN 562 566 HeLa HeLa NNP 567 569 S3 S3 NNP 570 575 cells cell NNS 575 576 . . . 577 581 From from IN 582 586 HeLa HeLa NNP 587 592 cells cell NNS 592 593 , , , 594 597 two two CD 598 603 major major JJ 604 612 proteins protein NNS 613 615 of of IN 616 618 52 52 CD 619 622 and and CC 623 625 45 45 CD 626 637 kilodaltons kilodalton NNS 638 639 ( ( ( 639 641 kD kd NN 641 642 ) ) ) 643 653 copurified copurifie VBN 654 658 with with IN 659 662 DNA DNA NNP 663 670 binding binding NN 671 679 activity activity NN 679 680 , , , 681 688 whereas whereas IN 689 693 from from IN 694 701 T-cells T-cell NNP 701 702 , , , 703 707 four four CD 708 719 proteins–a proteins–a NN 720 725 major major JJ 726 733 protein protein NN 734 736 of of IN 737 739 52 52 CD 740 742 kD kd NN 743 746 and and CC 747 752 three three CD 753 758 minor minor JJ 759 767 proteins protein NNS 768 770 of of IN 771 773 82 82 CD 773 774 , , , 775 777 67 67 CD 777 778 , , , 779 782 and and CC 783 788 43-47 43-47 CD 789 797 kD–were kd–were NN 798 806 purified purify VBN 806 807 . . . 808 812 Also also RB 812 813 , , , 814 816 an an DT 817 826 induction induction NN 827 835 specific specific JJ 836 839 DNA dna NN 840 847 binding binding NN 848 855 protein protein NN 856 859 was be VBD 860 868 purified purify VBN 869 873 from from IN 874 878 HeLa HeLa NNP 879 884 cells cell NNS 885 889 that that WDT 890 900 interacted interact VBD 901 905 with with IN 906 909 the the DT 910 911 ( ( ( 911 919 AAGTGA)4 aagtga)4 NN 920 932 tetrahexamer tetrahexamer NN 933 941 sequence sequence NN 942 945 and and CC 946 949 the the DT 950 954 PRDI PRDI NNP 955 961 domain domain NN 961 962 . . . 963 967 This this DT 968 975 protein protein NN 976 978 is be VBZ 979 994 immunologically immunologically RB 995 1003 distinct distinct JJ 1004 1008 from from IN 1009 1020 IRF-1/ISGF2 irf-1/isgf2 NN 1020 1021 . . . 1022 1031 Uninduced Uninduced NNP 1032 1034 or or CC 1035 1041 Sendai Sendai NNP 1042 1047 virus virus NN 1048 1055 induced induce VBD 1056 1060 HeLa HeLa NNP 1061 1069 extracts extract NNS 1070 1074 were be VBD 1075 1079 used use VBN 1080 1082 to to TO 1083 1090 examine examine VB 1091 1104 transcription transcription NN 1105 1107 in in FW 1108 1113 vitro vitro FW 1114 1119 using use VBG 1120 1121 a a DT 1122 1128 series series NN 1129 1131 of of IN 1132 1135 IFN ifn NN 1136 1140 beta beta NN 1141 1149 promoter promoter NN 1150 1159 deletions deletion NNS 1159 1160 . . . 1161 1170 Deletions deletion NNS 1171 1179 upstream upstream RB 1180 1182 of of IN 1183 1186 the the DT 1187 1192 PRDII PRDII NNP 1193 1200 element element NN 1201 1210 increased increase VBD 1211 1224 transcription transcription NN 1225 1227 in in IN 1228 1231 the the DT 1232 1241 uninduced uninduced JJ 1242 1249 extract extract NN 1249 1250 , , , 1251 1261 indicating indicate VBG 1262 1275 predominantly predominantly RB 1276 1284 negative negative JJ 1285 1295 regulation regulation NN 1296 1298 of of IN 1299 1302 the the DT 1303 1311 promoter promoter NN 1311 1312 . . . 1313 1314 A a DT 1315 1323 2-4-fold 2-4-fold JJ 1324 1332 increase increase NN 1333 1335 in in IN 1336 1344 IFN-beta IFN-beta NNP 1345 1353 promoter promoter NN 1354 1367 transcription transcription NN 1368 1371 was be VBD 1372 1380 observed observe VBN 1381 1383 in in IN 1384 1390 Sendai Sendai NNP 1391 1396 virus virus NN 1397 1404 induced induce VBN 1405 1413 extracts extract NNS 1413 1414 , , , 1415 1418 and and CC 1419 1427 deletion deletion NN 1428 1430 of of IN 1431 1435 PRDI PRDI NNP 1436 1439 and and CC 1440 1445 PRDII PRDII NNP 1446 1454 elements element NNS 1455 1464 decreased decrease VBD 1465 1469 this this DT 1470 1477 induced induce VBN 1478 1483 level level NN 1484 1486 of of IN 1487 1500 transcription transcription NN 1500 1501 . . . 1502 1506 When when WRB 1507 1515 purified purify VBN 1516 1521 PRDII PRDII NNP 1522 1525 and and CC 1526 1538 tetrahexamer tetrahexamer NN 1539 1546 binding binding NN 1547 1555 proteins protein NNS 1556 1560 were be VBD 1561 1566 added add VBN 1567 1569 to to TO 1570 1573 the the DT 1574 1581 induced induce VBN 1582 1589 extract extract NN 1589 1590 , , , 1591 1592 a a DT 1593 1599 4-fold 4-fold JJ 1600 1608 increase increase NN 1609 1611 in in IN 1612 1625 transcription transcription NN 1626 1629 was be VBD 1630 1638 observed observe VBN 1638 1639 . . . 1640 1645 These these DT 1646 1657 experiments experiment NNS 1658 1669 demonstrate demonstrate VBP 1670 1674 that that IN 1675 1677 it it PRP 1678 1680 is be VBZ 1681 1689 possible possible JJ 1690 1692 to to TO 1693 1701 modulate modulate VB 1702 1710 IFN-beta IFN-beta NNP 1711 1724 transcription transcription NN 1725 1727 in in FW 1728 1733 vitro vitro FW 1734 1737 but but CC 1738 1746 indicate indicate VBP 1747 1751 that that IN 1752 1762 additional additional JJ 1763 1771 proteins protein NNS 1772 1775 may may MD 1776 1778 be be VB 1779 1787 required require VBN 1788 1790 to to TO 1791 1796 fully fully RB 1797 1805 activate activate VB 1806 1814 IFN-beta IFN-beta NNP 1815 1828 transcription transcription NN 1828 1829 . . .