0 9 Isolation isolation NN 10 12 of of IN 13 14 a a DT 15 26 rel-related rel-related JJ 27 32 human human JJ 33 37 cDNA cdna NN 38 42 that that WDT 43 54 potentially potentially RB 55 62 encodes encode VBZ 63 66 the the DT 67 72 65-kD 65-kd JJ 73 80 subunit subunit NN 81 83 of of IN 84 92 NF-kappa NF-kappa NNP 93 94 B B NNP 95 96 [ [ ( 96 105 published publish VBN 106 113 erratum erratum NN 114 121 appears appear VBZ 122 124 in in IN 125 132 Science Science NNP 133 137 1991 1991 CD 138 141 Oct Oct NNP 142 156 4;254(5028):11 4;254(5028):11 CD 156 157 ] ] ) 159 160 A a NN 161 164 DNA dna NN 165 170 probe probe NN 171 175 that that WDT 176 183 spanned span VBD 184 185 a a DT 186 192 domain domain NN 193 202 conserved conserve VBN 203 208 among among IN 209 212 the the DT 213 227 proto-oncogene proto-oncogene NN 228 233 c-rel c-rel NN 233 234 , , , 235 238 the the DT 239 249 Drosophila Drosophila NNP 250 259 morphogen morphogen NN 260 266 dorsal dorsal NN 266 267 , , , 268 271 and and CC 272 275 the the DT 276 279 p50 p50 NN 280 283 DNA dna NN 284 291 binding binding NN 292 299 subunit subunit NN 300 302 of of IN 303 311 NF-kappa NF-kappa NNP 312 313 B B NNP 314 317 was be VBD 318 327 generated generate VBN 328 332 from from IN 333 339 Jurkat Jurkat NNP 340 341 T t NN 342 346 cell cell NN 347 360 complementary complementary JJ 361 364 DNA dna NN 365 369 with with IN 370 373 the the DT 374 384 polymerase polymerase NN 385 390 chain chain NN 391 399 reaction reaction NN 400 401 ( ( ( 401 404 PCR PCR NNP 404 405 ) ) ) 406 409 and and CC 410 420 degenerate degenerate JJ 421 437 oligonucleotides oligonucleotide NNS 437 438 . . . 439 443 This this DT 444 449 probe probe NN 450 453 was be VBD 454 458 used use VBN 459 461 to to TO 462 470 identify identify VB 471 472 a a DT 473 484 rel-related rel-related JJ 485 498 complementary complementary JJ 499 502 DNA dna NN 503 507 that that WDT 508 518 hybridized hybridize VBD 519 521 to to TO 522 523 a a DT 524 536 2.6-kilobase 2.6-kilobase JJ 537 546 messenger messenger NN 547 550 RNA RNA NNP 551 558 present present JJ 559 561 in in IN 562 567 human human JJ 568 569 T t NN 570 573 and and CC 574 575 B b NN 576 587 lymphocytes lymphocyte NNS 587 588 . . . 589 591 In in FW 592 597 vitro vitro FW 598 611 transcription transcription NN 612 615 and and CC 616 627 translation translation NN 628 630 of of IN 631 634 the the DT 635 648 complementary complementary JJ 649 652 DNA dna NN 653 661 resulted result VBD 662 664 in in IN 665 668 the the DT 669 678 synthesis synthesis NN 679 681 of of IN 682 683 a a DT 684 691 protein protein NN 692 696 with with IN 697 699 an an DT 700 708 apparent apparent JJ 709 718 molecular molecular JJ 719 723 size size NN 724 726 of of IN 727 729 65 65 CD 730 741 kilodaltons kilodalton NNS 742 743 ( ( ( 743 745 kD kd NN 745 746 ) ) ) 746 747 . . . 748 751 The the DT 752 762 translated translate VBN 763 770 protein protein NN 771 777 showed show VBD 778 782 weak weak JJ 783 786 DNA dna NN 787 794 binding binding NN 795 799 with with IN 800 801 a a DT 802 813 specificity specificity NN 814 817 for for IN 818 821 the the DT 822 827 kappa kappa NN 828 829 B B NNP 830 837 binding binding NN 838 843 motif motif NN 843 844 . . . 845 849 This this DT 850 861 protein-DNA protein-dna JJ 862 869 complex complex NN 870 880 comigrated comigrate VBD 881 885 with with IN 886 889 the the DT 890 897 complex complex NN 898 906 obtained obtain VBN 907 911 with with IN 912 915 the the DT 916 924 purified purify VBN 925 930 human human JJ 931 934 p65 p65 NN 935 943 NF-kappa NF-kappa NNP 944 945 B B NNP 946 953 subunit subunit NN 954 957 and and CC 958 965 binding binding NN 966 969 was be VBD 970 979 inhibited inhibit VBN 980 982 by by IN 983 984 I i NN 985 990 kappa kappa NN 991 998 B-alpha B-alpha NNP 999 1002 and and CC 1003 1008 -beta -beta NN 1009 1017 proteins protein NNS 1017 1018 . . . 1019 1021 In in IN 1022 1030 addition addition NN 1030 1031 , , , 1032 1035 the the DT 1036 1041 65-kD 65-kd JJ 1042 1049 protein protein NN 1050 1060 associated associate VBN 1061 1065 with with IN 1066 1069 the the DT 1070 1073 p50 p50 NN 1074 1081 subunit subunit NN 1082 1084 of of IN 1085 1093 NF-kappa NF-kappa NNP 1094 1095 B B NNP 1096 1099 and and CC 1100 1103 the the DT 1104 1109 kappa kappa NN 1110 1111 B B NNP 1112 1117 probe probe NN 1118 1120 to to TO 1121 1125 form form VB 1126 1127 a a DT 1128 1135 complex complex NN 1136 1140 with with IN 1141 1144 the the DT 1145 1149 same same JJ 1150 1165 electrophoretic electrophoretic JJ 1166 1174 mobility mobility NN 1175 1177 as as IN 1178 1181 the the DT 1182 1190 NF-kappa NF-kappa NNP 1191 1196 B-DNA B-DNA NNP 1197 1204 complex complex NN 1204 1205 . . . 1206 1215 Therefore therefore RB 1216 1219 the the DT 1220 1231 rel-related rel-related JJ 1232 1237 65-kD 65-kd JJ 1238 1245 protein protein NN 1246 1249 may may MD 1250 1259 represent represent VB 1260 1263 the the DT 1264 1267 p65 p65 NN 1268 1275 subunit subunit NN 1276 1278 of of IN 1279 1282 the the DT 1283 1289 active active JJ 1290 1298 NF-kappa NF-kappa NNP 1299 1300 B B NNP 1301 1314 transcription transcription NN 1315 1321 factor factor NN 1322 1329 complex complex NN 1329 1330 . . .