0	5	Human	human	JJ
6	11	tumor	tumor	NN
12	20	necrosis	necrosis	NN
21	27	factor	factor	NN
28	33	alpha	alpha	NN
34	38	gene	gene	NN
39	49	regulation	regulation	NN
50	52	in	in	IN
53	60	phorbol	phorbol	NN
61	66	ester	ester	NN
67	77	stimulated	stimulate	VBD
78	79	T	t	NN
80	83	and	and	CC
84	85	B	B	NNP
86	90	cell	cell	NN
91	96	lines	line	NNS
96	97	.	.	.

99	102	The	the	DT
103	110	minimal	minimal	JJ
111	117	region	region	NN
118	120	of	of	IN
121	124	the	the	DT
125	130	human	human	JJ
131	136	tumor	tumor	NN
137	145	necrosis	necrosis	NN
146	152	factor	factor	NN
153	158	alpha	alpha	NN
159	160	(	(	(
160	169	TNF-alpha	TNF-alpha	NNP
169	170	)	)	)
171	175	gene	gene	NN
176	184	promoter	promoter	NN
185	194	necessary	necessary	JJ
195	198	for	for	IN
199	202	its	its	PRP$
203	218	transcriptional	transcriptional	JJ
219	228	induction	induction	NN
229	231	by	by	IN
232	239	phorbol	phorbol	NN
240	246	esters	ester	NNS
247	248	(	(	(
248	251	PMA	PMA	NNP
251	252	)	)	)
253	255	in	in	IN
256	261	human	human	JJ
262	263	T	t	NN
264	267	and	and	CC
268	269	B	B	NNP
270	280	lymphocyte	lymphocyte	NN
281	285	cell	cell	NN
286	291	lines	line	NNS
292	295	has	have	VBZ
296	300	been	be	VBN
301	310	localized	localized	JJ
311	318	between	between	IN
319	322	-52	-52	CD
323	326	and	and	CC
327	330	+89	+89	CD
331	342	nucleotides	nucleotide	NNS
343	344	(	(	(
344	346	nt	nt	NN
346	347	)	)	)
348	356	relative	relative	JJ
357	359	to	to	TO
360	363	the	the	DT
364	368	gene	gene	NN
368	370	’s	's	POS
371	386	transcriptional	transcriptional	JJ
387	392	start	start	NN
393	397	site	site	NN
397	398	.	.	.

399	409	Comparison	comparison	NN
410	412	of	of	IN
413	418	these	these	DT
419	428	sequences	sequence	NNS
429	431	to	to	TO
432	437	those	those	DT
438	446	required	require	VBN
447	449	to	to	TO
450	457	mediate	mediate	VB
458	463	virus	virus	NN
464	466	or	or	CC
467	485	lipopolysaccharide	lipopolysaccharide	NN
486	487	(	(	(
487	490	LPS	LPS	NNP
490	491	)	)	)
492	501	induction	induction	NN
502	504	of	of	IN
505	508	the	the	DT
509	513	gene	gene	NN
514	520	reveal	reveal	VBP
521	532	significant	significant	JJ
533	544	differences	difference	NNS
544	545	,	,	,
546	549	and	and	CC
550	554	thus	thus	RB
554	555	,	,	,
556	559	the	the	DT
560	568	sequence	sequence	NN
569	581	requirements	requirement	NNS
582	585	for	for	IN
586	589	PMA	PMA	NNP
590	599	induction	induction	NN
600	603	are	be	VBP
604	612	distinct	distinct	JJ
613	617	from	from	IN
618	623	those	those	DT
624	628	that	that	WDT
629	636	mediate	mediate	VBP
637	646	induction	induction	NN
647	649	by	by	IN
650	655	virus	virus	NN
656	658	or	or	CC
659	662	LPS	lps	NN
662	663	.	.	.

664	672	Although	although	IN
673	678	three	three	CD
679	684	sites	site	NNS
685	687	in	in	IN
688	691	the	the	DT
692	701	TNF-alpha	TNF-alpha	NNP
702	710	promoter	promoter	NN
711	712	(	(	(
712	717	kappa	kappa	NN
718	719	1	1	CD
719	720	,	,	,
721	726	kappa	kappa	NN
727	728	2	2	CD
728	729	,	,	,
730	733	and	and	CC
734	739	kappa	kappa	NN
740	741	3	3	CD
741	742	)	)	)
743	755	specifically	specifically	RB
756	760	bind	bind	VBP
761	764	the	the	DT
765	778	transcription	transcription	NN
779	785	factor	factor	NN
786	794	NF-kappa	NF-kappa	NNP
795	796	B	B	NNP
797	799	in	in	IN
800	808	lymphoid	lymphoid	JJ
809	816	nuclear	nuclear	JJ
817	825	extracts	extract	NNS
825	826	,	,	,
827	836	TNF-alpha	TNF-alpha	NNP
837	841	mRNA	mRNA	NNP
842	851	induction	induction	NN
852	854	by	by	IN
855	858	PMA	PMA	NNP
859	863	does	do	VBZ
864	867	not	not	RB
868	877	correlate	correlate	VB
878	882	with	with	IN
883	891	NF-kappa	NF-kappa	NNP
892	893	B	B	NNP
894	901	binding	binding	NN
902	912	activities	activity	NNS
913	922	displayed	display	VBN
923	925	by	by	IN
926	935	different	different	JJ
936	937	T	t	NN
938	941	and	and	CC
942	943	B	B	NNP
944	948	cell	cell	NN
949	954	lines	line	NNS
954	955	.	.	.

956	964	Moreover	moreover	RB
964	965	,	,	,
966	971	kappa	kappa	NN
972	979	1-kappa	1-kappa	NN
980	981	3	3	CD
982	985	can	can	MD
986	990	each	each	DT
991	993	be	be	VB
994	1001	deleted	delete	VBN
1002	1006	from	from	IN
1007	1010	the	the	DT
1011	1020	TNF-alpha	TNF-alpha	NNP
1021	1029	promoter	promoter	NN
1030	1034	with	with	IN
1035	1041	little	little	JJ
1042	1048	effect	effect	NN
1049	1051	on	on	IN
1052	1055	the	the	DT
1056	1060	gene	gene	NN
1060	1062	’s	's	POS
1063	1075	inducibility	inducibility	NN
1076	1078	by	by	IN
1079	1082	PMA	PMA	NNP
1082	1083	.	.	.

1084	1093	Therefore	therefore	RB
1093	1094	,	,	,
1095	1104	TNF-alpha	TNF-alpha	NNP
1105	1109	mRNA	mRNA	NNP
1110	1119	induction	induction	NN
1120	1122	by	by	IN
1123	1126	PMA	PMA	NNP
1126	1127	,	,	,
1128	1132	like	like	IN
1133	1136	its	its	PRP$
1137	1146	induction	induction	NN
1147	1149	by	by	IN
1150	1155	virus	virus	NN
1156	1159	and	and	CC
1160	1163	LPS	LPS	NNP
1163	1164	,	,	,
1165	1167	is	be	VBZ
1168	1171	not	not	RB
1172	1181	primarily	primarily	RB
1182	1190	mediated	mediate	VBN
1191	1193	by	by	IN
1194	1202	NF-kappa	NF-kappa	NNP
1203	1204	B	B	NNP
1204	1205	,	,	,
1206	1209	but	but	CC
1210	1216	rather	rather	RB
1217	1219	is	be	VBZ
1220	1228	mediated	mediate	VBN
1229	1236	through	through	IN
1237	1242	other	other	JJ
1243	1252	sequences	sequence	NNS
1253	1256	and	and	CC
1257	1264	protein	protein	NN
1265	1272	factors	factor	NNS
1272	1273	.	.	.

1274	1286	Surprisingly	surprisingly	RB
1286	1287	,	,	,
1288	1297	multimers	multimer	NNS
1298	1300	of	of	IN
1301	1306	kappa	kappa	NN
1307	1314	1-kappa	1-kappa	NN
1315	1316	3	3	CD
1317	1320	can	can	MD
1321	1327	confer	confer	VB
1328	1331	PMA	PMA	NNP
1332	1344	inducibility	inducibility	NN
1345	1347	on	on	IN
1348	1349	a	a	DT
1350	1362	heterologous	heterologous	JJ
1363	1371	promoter	promoter	NN
1372	1374	in	in	IN
1375	1376	a	a	DT
1377	1378	B	B	NNP
1379	1380	(	(	(
1380	1384	Raji	Raji	NNP
1384	1385	)	)	)
1385	1386	,	,	,
1387	1390	but	but	CC
1391	1394	not	not	RB
1395	1396	a	a	DT
1397	1398	T	t	NN
1399	1400	(	(	(
1400	1405	HUT78	HUT78	NNP
1405	1406	)	)	)
1407	1411	cell	cell	NN
1412	1416	line	line	NN
1416	1417	.	.	.

1418	1425	However	however	RB
1426	1430	they	they	PRP
1431	1434	are	be	VBP
1435	1438	not	not	RB
1439	1449	functional	functional	JJ
1450	1452	on	on	IN
1453	1454	a	a	DT
1455	1464	truncated	truncate	VBN
1465	1474	TNF-alpha	TNF-alpha	NNP
1475	1483	promoter	promoter	NN
1483	1484	,	,	,
1485	1495	indicating	indicate	VBG
1496	1500	that	that	IN
1501	1509	promoter	promoter	NN
1510	1517	context	context	NN
1518	1521	and	and	CC
1522	1526	cell	cell	NN
1527	1531	type	type	NN
1532	1543	specificity	specificity	NN
1544	1553	influence	influence	VBP
1554	1557	the	the	DT
1558	1561	PMA	PMA	NNP
1562	1571	inducible	inducible	JJ
1572	1580	function	function	NN
1581	1583	of	of	IN
1584	1589	these	these	DT
1590	1598	NF-kappa	NF-kappa	NNP
1599	1600	B	B	NNP
1601	1608	binding	binding	NN
1609	1614	sites	site	NNS
1614	1615	.	.	.