0 12 Contribution contribution NN 13 15 of of IN 16 24 NF-kappa NF-kappa NNP 25 26 B B NNP 27 30 and and CC 31 34 Sp1 sp1 NN 35 42 binding binding NN 43 49 motifs motif NNS 50 52 to to TO 53 56 the the DT 57 68 replicative replicative JJ 69 77 capacity capacity NN 78 80 of of IN 81 86 human human JJ 87 103 immunodeficiency immunodeficiency NN 104 109 virus virus NN 110 114 type type NN 115 116 1 1 CD 116 117 : : : 118 126 distinct distinct JJ 127 135 patterns pattern NNS 136 138 of of IN 139 144 viral viral JJ 145 151 growth growth NN 152 155 are be VBP 156 166 determined determine VBN 167 169 by by IN 170 176 T-cell t-cell NN 177 182 types type NNS 182 183 . . . 185 193 Starting start VBG 194 198 with with IN 199 200 a a DT 201 224 replication-incompetent replication-incompetent JJ 225 234 molecular molecular JJ 235 240 clone clone NN 241 243 of of IN 244 249 human human JJ 250 266 immunodeficiency immunodeficiency NN 267 272 virus virus NN 273 277 type type NN 278 279 1 1 CD 279 280 , , , 281 288 lacking lack VBG 289 292 all all PDT 293 296 the the DT 297 305 NF-kappa NF-kappa NNP 306 307 B B NNP 308 311 and and CC 312 315 Sp1 sp1 NN 316 323 binding binding NN 324 329 sites site NNS 330 337 present present JJ 338 340 in in IN 341 344 the the DT 345 351 native native JJ 352 356 long long JJ 357 365 terminal terminal JJ 366 372 repeat repeat NN 373 374 ( ( ( 374 377 LTR LTR NNP 377 378 ) ) ) 378 379 , , , 380 390 proviruses provirus NNS 391 401 containing contain VBG 402 415 reconstructed reconstruct VBN 416 420 LTRs ltr NNS 421 425 with with IN 426 436 individual individual JJ 437 439 or or CC 440 452 combinations combination NNS 453 455 of of IN 456 464 NF-kappa NF-kappa NNP 465 466 B B NNP 467 470 and and CC 471 474 Sp1 Sp1 NNP 475 483 elements element NNS 484 488 were be VBD 489 498 generated generate VBN 499 502 and and CC 503 512 evaluated evaluate VBN 513 516 for for IN 517 522 their their PRP$ 523 531 capacity capacity NN 532 534 to to TO 535 542 produce produce VB 543 548 virus virus NN 549 556 progeny progeny NN 557 566 following follow VBG 567 593 transfection-cocultivation transfection-cocultivation NN 593 594 . . . 595 600 Virus virus NN 601 607 stocks stock NNS 608 616 obtained obtain VBN 617 621 from from IN 622 627 these these DT 628 639 experiments experiment NNS 640 649 exhibited exhibit VBD 650 651 a a DT 652 661 continuum continuum NN 662 664 of of IN 665 676 replicative replicative JJ 677 687 capacities capacity NNS 688 690 in in IN 691 700 different different JJ 701 706 human human JJ 707 713 T-cell t-cell NN 714 719 types type NNS 720 729 depending depend VBG 730 732 on on IN 733 738 which which WDT 739 749 element(s) element(s) NN 750 753 was be VBD 754 761 present present JJ 762 764 in in IN 765 768 the the DT 769 772 LTR ltr NN 772 773 . . . 774 777 For for IN 778 785 example example NN 785 786 , , , 787 789 in in IN 790 801 experiments experiment NNS 802 811 involving involve VBG 812 820 proviral proviral JJ 821 827 clones clone NNS 828 832 with with IN 833 837 LTRs ltr NNS 838 848 containing contain VBG 849 852 one one CD 853 855 or or CC 856 859 two two CD 860 868 NF-kappa NF-kappa NNP 869 870 B B NNP 871 879 elements element NNS 880 881 ( ( ( 881 884 and and CC 885 887 no no DT 888 891 Sp1 sp1 NN 892 899 binding binding NN 900 905 sites site NNS 905 906 ) ) ) 906 907 , , , 908 909 a a DT 910 919 hierarchy hierarchy NN 920 922 of of IN 923 931 cellular cellular JJ 932 944 permissivity permissivity NN 945 947 to to TO 948 953 virus virus NN 954 965 replication replication NN 966 967 ( ( ( 967 977 peripheral peripheral JJ 978 983 blood blood NN 984 995 lymphocytes lymphocyte NNS 996 997 = = SYM 998 1001 MT4 mt4 NN 1002 1009 greater great JJR 1010 1014 than than IN 1015 1017 H9 H9 NNP 1018 1025 greater great JJR 1026 1030 than than IN 1031 1034 CEM CEM NNP 1035 1042 greater great JJR 1043 1047 than than IN 1048 1054 Jurkat Jurkat NNP 1054 1055 ) ) ) 1056 1059 was be VBD 1060 1068 observed observe VBN 1068 1069 . . . 1070 1072 Of of IN 1073 1077 note note NN 1078 1081 was be VBD 1082 1085 the the DT 1086 1096 associated associate VBN 1097 1106 emergence emergence NN 1107 1109 of of IN 1110 1121 second-site second-site JJ 1122 1125 LTR ltr NN 1126 1136 revertants revertant NNS 1137 1142 which which WDT 1143 1151 involved involve VBD 1152 1154 an an DT 1155 1165 alteration alteration NN 1166 1168 of of IN 1169 1172 the the DT 1173 1177 TATA TATA NNP 1178 1181 box box NN 1181 1182 . . . 1183 1188 These these DT 1189 1196 results result NNS 1197 1204 suggest suggest VBP 1205 1209 that that IN 1210 1213 the the DT 1214 1219 human human JJ 1220 1236 immunodeficiency immunodeficiency NN 1237 1242 virus virus NN 1243 1247 type type NN 1248 1249 1 1 CD 1250 1253 LTR ltr NN 1254 1263 possesses possess VBZ 1264 1274 functional functional JJ 1275 1285 redundancy redundancy NN 1286 1291 which which WDT 1292 1299 ensures ensure VBZ 1300 1305 virus virus NN 1306 1317 replication replication NN 1318 1320 in in IN 1321 1330 different different JJ 1331 1337 T-cell t-cell NN 1338 1343 types type NNS 1344 1347 and and CC 1348 1350 is be VBZ 1351 1358 capable capable JJ 1359 1361 of of IN 1362 1370 changing change VBG 1371 1380 depending depend VBG 1381 1383 on on IN 1384 1387 the the DT 1388 1398 particular particular JJ 1399 1410 combination combination NN 1411 1413 of of IN 1414 1429 transcriptional transcriptional JJ 1430 1437 factors factor NNS 1438 1445 present present JJ 1445 1446 . . .