0 1 A a DT 2 7 novel novel JJ 8 14 T-cell t-cell NN 15 30 trans-activator trans-activator NN 31 35 that that WDT 36 46 recognizes recognize VBZ 47 48 a a DT 49 56 phorbol phorbol NN 57 72 ester-inducible ester-inducible JJ 73 80 element element NN 81 83 of of IN 84 87 the the DT 88 101 interleukin-2 interleukin-2 NN 102 110 promoter promoter NN 110 111 . . . 113 116 The the DT 117 128 interleukin interleukin NN 129 130 2 2 CD 131 132 ( ( ( 132 136 IL-2 il-2 NN 136 137 ) ) ) 138 142 gene gene NN 143 151 promoter promoter NN 152 154 is be VBZ 155 165 recognized recognize VBN 166 168 by by IN 169 176 several several JJ 177 195 cell-type-specific cell-type-specific JJ 196 199 and and CC 200 210 ubiquitous ubiquitous JJ 211 226 transcriptional transcriptional JJ 227 237 regulators regulator NNS 238 242 that that WDT 243 252 integrate integrate VBP 253 264 information information NN 265 276 transmitted transmit VBN 277 279 by by IN 280 287 various various JJ 288 297 signaling signaling NN 298 305 systems system NNS 306 313 leading lead VBG 314 316 to to TO 317 321 IL-2 il-2 NN 322 332 production production NN 333 336 and and CC 337 343 T-cell t-cell NN 344 354 activation activation NN 354 355 . . . 356 361 Using use VBG 362 363 a a DT 364 375 combination combination NN 376 378 of of IN 379 391 transfection transfection NN 391 392 , , , 393 404 protein-DNA protein-dna JJ 405 412 binding binding NN 412 413 , , , 414 417 and and CC 418 420 in in FW 421 426 vitro vitro FW 427 440 transcription transcription NN 441 448 methods method NNS 448 449 , , , 450 452 we we PRP 453 457 have have VBP 458 468 discovered discover VBN 469 472 the the DT 473 478 novel novel JJ 479 494 T-cell-specific t-cell-specific JJ 495 510 transcriptional transcriptional JJ 511 520 activator activator NN 521 526 TCF-1 tcf-1 NN 527 528 ( ( ( 528 531 for for IN 532 538 T-Cell T-Cell NNP 539 547 Factor-1 Factor-1 NNP 547 548 ) ) ) 548 549 , , , 550 555 which which WDT 556 566 recognizes recognize VBZ 567 568 a a DT 569 584 T-cell-specific t-cell-specific JJ 585 593 response response NN 594 601 element element NN 602 603 ( ( ( 603 606 TCE TCE NNP 606 607 ) ) ) 608 615 located located JJ 616 622 within within IN 623 626 the the DT 627 631 IL-2 il-2 NN 632 640 promoter promoter NN 640 641 . . . 642 650 Although although IN 651 654 the the DT 655 658 TCE tce NN 659 661 is be VBZ 662 669 similar similar JJ 670 672 in in IN 673 681 sequence sequence NN 682 684 to to TO 685 686 a a DT 687 696 consensus consensus NN 697 699 NF NF NNP 700 705 kappa kappa NN 706 707 B B NNP 708 712 site site NN 712 713 , , , 714 721 several several JJ 722 730 criteria criterion NNS 731 739 indicate indicate VBP 740 744 that that IN 745 750 TCF-1 tcf-1 NN 751 753 is be VBZ 754 762 distinct distinct JJ 763 767 from from IN 768 770 NF NF NNP 771 776 kappa kappa NN 777 779 B. B. NNP 780 787 However however RB 787 788 , , , 789 793 like like IN 794 796 NF NF NNP 797 802 kappa kappa NN 803 804 B B NNP 804 805 , , , 806 811 TCF-1 tcf-1 NN 812 820 activity activity NN 821 823 is be VBZ 824 831 induced induce VBN 832 834 by by IN 835 842 phorbol phorbol NN 843 849 esters ester NNS 850 853 and and CC 854 859 other other JJ 860 866 T-cell t-cell NN 867 877 activators activator NNS 877 878 . . .