0 14 Identification identification NN 15 17 of of IN 18 19 a a DT 20 25 novel novel JJ 26 32 factor factor NN 33 37 that that WDT 38 47 interacts interact VBZ 48 52 with with IN 53 55 an an DT 56 70 immunoglobulin immunoglobulin NN 71 82 heavy-chain heavy-chain JJ 83 91 promoter promoter NN 92 95 and and CC 96 106 stimulates stimulate VBZ 107 120 transcription transcription NN 121 123 in in IN 124 135 conjunction conjunction NN 136 140 with with IN 141 144 the the DT 145 153 lymphoid lymphoid JJ 154 167 cell-specific cell-specific JJ 168 174 factor factor NN 175 179 OTF2 otf2 NN 179 180 . . . 182 185 The the DT 186 201 tissue-specific tissue-specific JJ 202 212 expression expression NN 213 215 of of IN 216 219 the the DT 220 224 MOPC MOPC NNP 225 228 141 141 CD 229 243 immunoglobulin immunoglobulin NN 244 255 heavy-chain heavy-chain JJ 256 260 gene gene NN 261 264 was be VBD 265 272 studied study VBN 273 275 by by IN 276 281 using use VBG 282 284 in in FW 285 290 vitro vitro FW 291 304 transcription transcription NN 304 305 . . . 306 321 B-cell-specific b-cell-specific JJ 322 335 transcription transcription NN 336 338 of of IN 339 343 this this DT 344 348 gene gene NN 349 352 was be VBD 353 362 dependent dependent JJ 363 365 on on IN 366 369 the the DT 370 377 octamer octamer NN 378 385 element element NN 386 399 5’-ATGCAAAG-3 5'-atgcaaag-3 NN 399 400 ’ ' '' 400 401 , , , 402 409 located located JJ 410 412 in in IN 413 416 the the DT 417 425 upstream upstream JJ 426 432 region region NN 433 435 of of IN 436 440 this this DT 441 449 promoter promoter NN 450 453 and and CC 454 456 in in IN 457 460 the the DT 461 470 promoters promoter NNS 471 473 of of IN 474 477 all all DT 478 483 other other JJ 484 498 immunoglobulin immunoglobulin NN 499 505 heavy- heavy- NN 506 509 and and CC 510 521 light-chain light-chain JJ 522 527 genes gene NNS 527 528 . . . 529 532 The the DT 533 544 interaction interaction NN 545 547 of of IN 548 556 purified purify VBN 557 564 octamer octamer NN 565 578 transcription transcription NN 579 586 factors factor NNS 587 588 1 1 CD 589 592 and and CC 593 594 2 2 CD 595 596 ( ( ( 596 600 OTF1 OTF1 NNP 601 604 and and CC 605 609 OTF2 OTF2 NNP 609 610 ) ) ) 611 615 with with IN 616 619 the the DT 620 624 MOPC MOPC NNP 625 628 141 141 CD 629 637 promoter promoter NN 638 641 was be VBD 642 649 studied study VBN 650 652 by by IN 653 658 using use VBG 659 674 electrophoretic electrophoretic JJ 675 683 mobility mobility NN 684 689 shift shift NN 690 696 assays assay NNS 697 700 and and CC 701 706 DNase dnase NN 707 708 I i CD 709 721 footprinting footprinting NN 721 722 . . . 723 731 Purified purify VBN 732 736 OTF1 OTF1 NNP 737 741 from from IN 742 746 HeLa HeLa NNP 747 752 cells cell NNS 753 756 and and CC 757 761 OTF1 otf1 NN 762 765 and and CC 766 770 OTF2 otf2 NN 771 775 from from IN 776 777 B B NNP 778 783 cells cell NNS 784 789 bound bind VBD 790 792 to to TO 793 802 identical identical JJ 803 812 sequences sequence NNS 813 819 within within IN 820 823 the the DT 824 835 heavy-chain heavy-chain JJ 836 844 promoter promoter NN 844 845 . . . 846 849 The the DT 850 853 OTF OTF NNP 854 866 interactions interaction NNS 867 869 we we PRP 870 878 observed observe VBD 879 887 extended extend VBN 888 892 over over IN 893 896 the the DT 897 905 heptamer heptamer NN 906 913 element element NN 914 926 5’-CTCAGGA-3 5'-ctcagga-3 NN 926 927 ’ ' '' 927 928 , , , 929 932 and and CC 933 935 it it PRP 936 941 seems seem VBZ 942 948 likely likely JJ 949 953 that that IN 954 957 the the DT 958 965 binding binding NN 966 968 of of IN 969 972 the the DT 973 981 purified purify VBN 982 989 factors factor NNS 990 998 involves involve VBZ 999 1010 cooperation cooperation NN 1011 1018 between between IN 1019 1026 octamer octamer NN 1027 1030 and and CC 1031 1039 heptamer heptamer NN 1040 1045 sites site NNS 1046 1048 in in IN 1049 1053 this this DT 1054 1062 promoter promoter NN 1062 1063 . . . 1064 1066 In in IN 1067 1075 addition addition NN 1076 1078 to to TO 1079 1084 these these DT 1085 1093 elements element NNS 1093 1094 , , , 1095 1097 we we PRP 1098 1108 identified identify VBD 1109 1110 a a DT 1111 1117 second second JJ 1118 1128 regulatory regulatory JJ 1129 1136 element element NN 1136 1137 , , , 1138 1141 the the DT 1142 1143 N N NNP 1144 1151 element element NN 1152 1156 with with IN 1157 1160 the the DT 1161 1169 sequence sequence NN 1170 1187 5’-GGAACCTCCCCC-3 5'-ggaacctccccc-3 NN 1187 1188 ’ ' '' 1188 1189 . . . 1190 1193 The the DT 1194 1195 N N NNP 1196 1203 element element NN 1204 1209 could could MD 1210 1223 independently independently RB 1224 1231 mediate mediate VB 1232 1235 low low JJ 1236 1242 levels level NNS 1243 1245 of of IN 1246 1259 transcription transcription NN 1260 1262 in in IN 1263 1267 both both CC 1268 1274 B-cell b-cell NN 1275 1278 and and CC 1279 1288 HeLa-cell hela-cell NN 1289 1297 extracts extract NNS 1297 1298 , , , 1299 1302 and and CC 1302 1303 , , , 1304 1306 in in IN 1307 1318 conjunction conjunction NN 1319 1323 with with IN 1324 1327 the the DT 1328 1335 octamer octamer NN 1336 1343 element element NN 1343 1344 , , , 1345 1347 it it PRP 1348 1351 can can MD 1352 1359 promote promote VB 1360 1364 high high JJ 1365 1371 levels level NNS 1372 1374 of of IN 1375 1388 transcription transcription NN 1389 1391 in in IN 1392 1398 B-cell b-cell NN 1399 1407 extracts extract NNS 1407 1408 . . . 1409 1412 The the DT 1413 1414 N N NNP 1415 1422 element element NN 1423 1428 bound bind VBD 1429 1430 a a DT 1431 1444 transcription transcription NN 1445 1451 factor factor NN 1451 1452 , , , 1453 1456 NTF NTF NNP 1456 1457 , , , 1458 1462 that that WDT 1463 1465 is be VBZ 1466 1476 ubiquitous ubiquitous JJ 1477 1479 in in IN 1480 1489 cell-type cell-type JJ 1490 1502 distribution distribution NN 1502 1503 , , , 1504 1507 and and CC 1508 1511 NTF NTF NNP 1512 1515 was be VBD 1516 1524 distinct distinct JJ 1525 1529 from from IN 1530 1533 any any DT 1534 1536 of of IN 1537 1540 the the DT 1541 1551 previously previously RB 1552 1561 described describe VBN 1562 1570 proteins protein NNS 1571 1575 that that WDT 1576 1580 bind bind VBP 1581 1583 to to TO 1584 1591 similar similar JJ 1592 1601 sequences sequence NNS 1601 1602 . . . 1603 1608 Based base VBN 1609 1611 on on IN 1612 1617 these these DT 1618 1625 results result NNS 1625 1626 , , , 1627 1629 we we PRP 1630 1637 propose propose VBP 1638 1642 that that IN 1643 1646 NTF NTF NNP 1647 1650 and and CC 1651 1655 OTF2 otf2 NN 1656 1668 interactions interaction NNS 1669 1670 ( ( ( 1670 1674 both both DT 1675 1679 with with IN 1680 1685 their their PRP$ 1686 1693 cognate cognate JJ 1694 1697 DNA dna NN 1698 1706 elements element NNS 1707 1710 and and CC 1711 1719 possibly possibly RB 1720 1722 at at IN 1723 1726 the the DT 1727 1742 protein-protein protein-protein JJ 1743 1748 level level NN 1748 1749 ) ) ) 1750 1753 may may MD 1754 1756 be be VB 1757 1765 critical critical JJ 1766 1768 to to TO 1769 1784 B-cell-specific b-cell-specific JJ 1785 1795 expression expression NN 1796 1799 and and CC 1800 1804 that that IN 1805 1810 these these DT 1811 1823 interactions interaction NNS 1824 1831 provide provide VBP 1832 1842 additional additional JJ 1843 1851 pathways pathway NNS 1852 1855 for for IN 1856 1866 regulating regulate VBG 1867 1871 gene gene NN 1872 1882 expression expression NN 1882 1883 . . .