0 3 The the DT 4 14 expression expression NN 15 17 of of IN 18 23 c-fos c-fos NN 23 24 , , , 25 30 c-jun c-jun NN 30 31 , , , 32 35 and and CC 36 41 c-myc c-myc NN 42 47 genes gene NNS 48 50 is be VBZ 51 60 regulated regulate VBN 61 63 by by IN 64 68 heat heat NN 69 74 shock shock NN 75 77 in in IN 78 83 human human JJ 84 92 lymphoid lymphoid JJ 93 98 cells cell NNS 98 99 . . . 101 104 The the DT 105 111 effect effect NN 112 114 of of IN 115 119 heat heat NN 120 125 shock shock NN 126 128 on on IN 129 132 the the DT 133 143 expression expression NN 144 146 of of IN 147 150 the the DT 151 158 nuclear nuclear JJ 159 173 protooncogenes protooncogene NNS 174 179 c-fos c-fos NN 179 180 , , , 181 186 c-jun c-jun NN 186 187 , , , 188 191 and and CC 192 197 c-myc c-myc NN 198 201 was be VBD 202 209 studied study VBN 210 212 in in IN 213 218 human human JJ 219 227 lymphoid lymphoid JJ 228 233 cells cell NNS 233 234 . . . 235 239 Heat Heat NNP 240 245 shock shock NN 246 252 caused cause VBD 253 255 an an DT 256 264 increase increase NN 265 267 in in IN 268 273 c-fos c-fos NN 274 277 and and CC 278 283 c-jun c-jun NN 284 288 mRNA mrna NN 289 295 levels level NNS 296 299 and and CC 300 301 a a DT 302 310 decrease decrease NN 311 313 in in IN 314 319 c-myc c-myc NN 320 324 mRNA mrna NN 325 331 levels level NNS 332 334 in in IN 335 340 pre-B pre-b JJ 341 342 ( ( ( 342 346 Hyon Hyon NNP 346 347 ) ) ) 348 351 and and CC 352 353 T t NN 354 355 ( ( ( 355 361 DND-41 dnd-41 NN 361 362 ) ) ) 363 367 cell cell NN 368 373 lines line NNS 374 376 as as RB 377 381 well well RB 382 384 as as IN 385 387 in in IN 388 395 freshly freshly RB 396 404 isolated isolate VBN 405 411 normal normal JJ 412 417 human human JJ 418 428 thymocytes thymocyte NNS 428 429 . . . 430 433 The the DT 434 441 changes change NNS 442 444 in in IN 445 448 the the DT 449 453 mRNA mrna NN 454 460 levels level NNS 461 463 of of IN 464 469 these these DT 470 484 protooncogenes protooncogene NNS 485 487 in in IN 488 492 Hyon Hyon NNP 493 498 cells cell NNS 499 503 were be VBD 504 508 most most RBS 509 519 pronounced pronounced JJ 520 522 at at IN 523 525 42 42 CD 526 529 and and CC 530 532 43 43 CD 533 540 degrees degree NNS 541 542 C c JJ 542 543 ; ; : 544 551 kinetic kinetic JJ 552 560 analysis analysis NN 561 573 demonstrated demonstrate VBD 574 578 that that IN 579 582 the the DT 583 590 changes change NNS 591 596 could could MD 597 599 be be VB 600 608 detected detect VBN 609 615 within within IN 616 618 30 30 CD 619 622 min min NN 623 625 of of IN 626 630 heat heat NN 631 636 shock shock NN 636 637 . . . 638 645 Altered altered JJ 646 659 transcription transcription NN 660 662 of of IN 663 668 c-fos c-fos NN 669 672 and and CC 673 678 c-myc c-myc NN 679 684 genes gene NNS 685 688 was be VBD 689 692 the the DT 693 700 primary primary JJ 701 707 effect effect NN 708 710 of of IN 711 715 heat heat NN 716 721 shock shock NN 721 722 . . . 723 734 Secondarily secondarily RB 734 735 , , , 736 740 heat heat NN 741 746 shock shock NN 747 749 of of IN 750 754 Hyon Hyon NNP 755 760 cells cell NNS 761 771 stabilized stabilize VBD 772 775 the the DT 776 781 c-myc c-myc NN 782 786 mRNA mrna NN 787 792 level level NN 793 795 by by IN 796 806 increasing increase VBG 807 810 its its PRP$ 811 820 half-life half-life NN 821 825 from from IN 826 828 24 24 CD 829 831 to to TO 832 834 45 45 CD 835 838 min min NN 838 839 . . . 840 843 The the DT 844 851 overall overall JJ 852 858 effect effect NN 859 861 of of IN 862 866 heat heat NN 867 872 shock shock NN 873 875 on on IN 876 881 c-myc c-myc NN 882 886 mRNA mrna NN 887 892 level level NN 892 893 , , , 894 901 however however RB 901 902 , , , 903 906 was be VBD 907 908 a a DT 909 915 marked marked JJ 916 926 inhibition inhibition NN 927 929 of of IN 930 933 its its PRP$ 934 947 transcription transcription NN 947 948 . . . 949 954 These these DT 955 962 results result NNS 963 974 demonstrate demonstrate VBP 975 979 that that IN 980 983 the the DT 984 997 transcription transcription NN 998 1000 of of IN 1001 1008 nuclear nuclear JJ 1009 1023 protooncogenes protooncogene NNS 1024 1026 is be VBZ 1027 1036 regulated regulate VBN 1037 1039 by by IN 1040 1044 heat heat NN 1045 1050 shock shock NN 1051 1061 indicating indicate VBG 1062 1063 a a DT 1064 1068 role role NN 1069 1072 for for IN 1073 1080 nuclear nuclear JJ 1081 1095 protooncogenes protooncogene NNS 1096 1098 in in IN 1099 1102 the the DT 1103 1109 stress stress NN 1110 1118 response response NN 1119 1121 of of IN 1122 1130 lymphoid lymphoid JJ 1131 1136 cells cell NNS 1136 1137 . . .