0 9 Induction Induction NNP 10 12 of of IN 13 22 immediate immediate JJ 23 28 early early JJ 29 37 response response NN 38 43 genes gene NNS 44 46 by by IN 47 57 macrophage macrophage NN 58 76 colony-stimulating colony-stimulating JJ 77 83 factor factor NN 84 86 in in IN 87 93 normal normal JJ 94 99 human human JJ 100 109 monocytes monocyte NNS 109 110 . . . 112 113 A a DT 114 119 group group NN 120 122 of of IN 123 135 coordinately coordinately RB 136 143 induced induce VBN 144 158 protooncogenes protooncogene NNS 158 159 , , , 160 172 cytoskeletal cytoskeletal JJ 172 173 , , , 174 177 and and CC 178 191 extracellular extracellular JJ 192 198 matrix matrix NN 199 204 genes gene NNS 205 209 have have VBP 210 214 been be VBN 215 221 termed term VBN 222 231 immediate immediate JJ 232 237 early early JJ 238 246 response response NN 247 252 genes gene NNS 252 253 , , , 254 257 and and CC 258 263 their their PRP$ 264 273 induction induction NN 274 277 has have VBZ 278 282 been be VBN 283 293 associated associate VBN 294 298 with with IN 299 305 growth growth NN 306 323 factor-stimulated factor-stimulated JJ 324 328 cell cell NN 329 342 proliferation proliferation NN 342 343 . . . 344 346 We we PRP 347 351 have have VBP 352 364 investigated investigate VBN 365 368 the the DT 369 378 induction induction NN 379 381 of of IN 382 387 these these DT 388 393 genes gene NNS 394 396 by by IN 397 411 macrophage-CSF macrophage-CSF NNP 412 413 ( ( ( 413 418 M-CSF M-CSF NNP 418 419 ) ) ) 420 422 in in IN 423 428 human human JJ 429 438 monocytes monocyte NNS 439 443 that that WDT 444 446 do do VBP 447 450 not not RB 451 462 proliferate proliferate VB 463 465 in in IN 466 474 response response NN 475 477 to to TO 478 483 M-CSF M-CSF NNP 484 487 but but CC 488 495 require require VBP 496 499 the the DT 500 506 factor factor NN 507 510 for for IN 511 518 optimal optimal JJ 519 523 cell cell NN 524 539 differentiation differentiation NN 539 540 . . . 541 547 Normal normal JJ 548 553 human human JJ 554 563 monocytes monocyte NNS 564 568 were be VBD 569 577 isolated isolate VBN 577 578 , , , 579 588 carefully carefully RB 589 595 washed wash VBN 595 596 , , , 597 600 and and CC 601 610 incubated incubate VBN 611 614 for for IN 615 617 36 36 CD 618 620 to to TO 621 623 48 48 CD 624 625 h h NN 626 628 in in IN 629 634 fetal fetal JJ 635 641 bovine bovine JJ 642 658 serum-containing serum-containing JJ 659 665 medium medium NN 665 666 . . . 667 669 At at IN 670 673 the the DT 674 677 end end NN 678 680 of of IN 681 685 this this DT 686 696 incubation incubation NN 697 700 the the DT 701 708 resting rest VBG 709 714 cells cell NNS 715 719 were be VBD 720 730 stimulated stimulate VBN 731 735 with with IN 736 741 M-CSF M-CSF NNP 741 742 , , , 743 746 and and CC 747 750 RNA RNA NNP 751 754 was be VBD 755 763 isolated isolate VBN 764 767 for for IN 768 776 analysis analysis NN 777 779 by by IN 780 788 Northern Northern NNP 789 797 blotting blotting NN 797 798 . . . 799 802 RNA RNA NNP 803 807 from from IN 808 815 control control NN 816 823 resting rest VBG 824 829 cells cell NNS 830 839 contained contain VBD 840 843 low low JJ 844 846 to to TO 847 859 undetectable undetectable JJ 860 866 levels level NNS 867 869 of of IN 870 875 c-jun c-jun NN 875 876 , , , 877 888 fibronectin fibronectin NN 889 897 receptor receptor NN 897 898 , , , 899 902 and and CC 903 908 actin actin NN 909 913 mRNA mrna NN 913 914 . . . 915 921 Within within IN 922 924 15 15 CD 925 927 to to TO 928 930 30 30 CD 931 934 min min NN 935 937 of of IN 938 946 addition addition NN 947 949 of of IN 950 955 M-CSF M-CSF NNP 955 956 , , , 957 964 however however RB 964 965 , , , 966 971 there there EX 972 975 was be VBD 976 977 a a DT 978 986 dramatic dramatic JJ 987 997 coordinate coordinate JJ 998 1007 induction induction NN 1008 1010 of of IN 1011 1016 these these DT 1017 1022 genes gene NNS 1022 1023 . . . 1024 1027 The the DT 1028 1033 c-jun c-jun NN 1034 1038 gene gene NN 1039 1049 expression expression NN 1050 1053 was be VBD 1054 1058 very very RB 1059 1068 transient transient JJ 1069 1072 and and CC 1073 1076 was be VBD 1077 1080 not not RB 1081 1091 detectable detectable JJ 1092 1094 by by IN 1095 1097 60 60 CD 1098 1101 min min NN 1102 1107 after after IN 1108 1113 M-CSF M-CSF NNP 1114 1122 addition addition NN 1122 1123 . . . 1124 1126 In in IN 1127 1135 contrast contrast NN 1135 1136 , , , 1137 1140 the the DT 1141 1151 expression expression NN 1152 1154 of of IN 1155 1160 actin actin NN 1161 1164 and and CC 1165 1176 fibronectin fibronectin NN 1177 1185 receptor receptor NN 1186 1190 mRNA mRNA NNP 1191 1194 was be VBD 1195 1199 more more RBR 1200 1209 sustained sustain VBN 1209 1210 , , , 1211 1214 and and CC 1215 1218 the the DT 1219 1229 expression expression NN 1230 1232 of of IN 1233 1238 these these DT 1239 1244 genes gene NNS 1245 1253 remained remain VBD 1254 1262 elevated elevated JJ 1263 1265 at at IN 1266 1268 24 24 CD 1269 1271 to to TO 1272 1274 48 48 CD 1275 1276 h h NN 1277 1282 after after IN 1283 1288 M-CSF M-CSF NNP 1289 1297 addition addition NN 1297 1298 . . . 1299 1301 We we PRP 1302 1306 also also RB 1307 1315 observed observe VBD 1316 1319 the the DT 1320 1329 induction induction NN 1330 1332 of of IN 1333 1336 the the DT 1337 1351 myelomonocytic myelomonocytic JJ 1352 1360 specific specific JJ 1361 1369 tyrosine tyrosine NN 1370 1376 kinase kinase NNP 1377 1380 hck hck NN 1381 1385 gene gene NN 1386 1400 simultaneously simultaneously RB 1401 1405 with with IN 1406 1409 the the DT 1410 1415 other other JJ 1416 1425 immediate immediate JJ 1426 1431 early early JJ 1432 1440 response response NN 1441 1446 genes gene NNS 1446 1447 . . . 1448 1451 The the DT 1452 1459 protein protein NN 1460 1469 synthesis synthesis NN 1470 1479 inhibitor inhibitor NN 1480 1493 cycloheximide cycloheximide NN 1494 1497 did do VBD 1498 1501 not not RB 1502 1507 block block VB 1508 1511 the the DT 1512 1521 induction induction NN 1522 1524 of of IN 1525 1528 any any DT 1529 1531 of of IN 1532 1537 these these DT 1538 1543 genes gene NNS 1543 1544 , , , 1545 1548 and and CC 1549 1551 in in IN 1552 1556 fact fact NN 1556 1557 , , , 1558 1571 super-induced super-induced JJ 1572 1575 the the DT 1576 1586 expression expression NN 1587 1589 of of IN 1590 1595 c-jun c-jun NN 1596 1599 and and CC 1600 1603 hck hck NN 1603 1604 . . . 1605 1612 Nuclear nuclear JJ 1613 1616 run run NN 1617 1619 on on IN 1620 1633 transcription transcription NN 1634 1636 of of IN 1637 1640 the the DT 1641 1646 c-jun c-jun NN 1646 1647 , , , 1648 1651 hck hck NN 1651 1652 , , , 1653 1656 and and CC 1657 1662 actin actin NN 1663 1668 genes gene NNS 1668 1669 . . . 1670 1679 Therefore therefore RB 1679 1680 , , , 1681 1683 in in IN 1684 1690 normal normal JJ 1691 1696 human human JJ 1697 1706 monocytes monocyte NNS 1707 1712 M-CSF M-CSF NNP 1713 1720 induces induce VBZ 1721 1730 immediate immediate JJ 1731 1736 early early JJ 1737 1745 response response NN 1746 1751 genes gene NNS 1752 1759 without without IN 1760 1768 inducing induce VBG 1769 1773 cell cell NN 1774 1787 proliferation proliferation NN 1787 1788 . . . 1789 1794 These these DT 1795 1800 genes gene NNS 1801 1804 may may MD 1805 1809 then then RB 1810 1814 play play VB 1815 1816 a a DT 1817 1821 role role NN 1822 1824 in in IN 1825 1833 altering alter VBG 1834 1837 the the DT 1838 1849 physiologic physiologic JJ 1850 1856 status status NN 1857 1859 of of IN 1860 1863 the the DT 1864 1869 cells cell NNS 1870 1872 in in IN 1873 1881 response response NN 1882 1884 to to TO 1885 1888 CSF CSF NNP 1888 1889 . . .