0 8 Lymphoid lymphoid JJ 9 17 specific specific JJ 18 22 gene gene NN 23 33 expression expression NN 34 36 of of IN 37 40 the the DT 41 51 adenovirus adenovirus NN 52 57 early early JJ 58 64 region region NN 65 66 3 3 CD 67 75 promoter promoter NN 76 78 is be VBZ 79 87 mediated mediate VBN 88 90 by by IN 91 99 NF-kappa NF-kappa NNP 100 101 B B NNP 102 109 binding binding NN 110 116 motifs motif NNS 116 117 . . . 119 120 A a DT 121 128 primary primary JJ 129 133 site site NN 134 136 of of IN 137 146 infection infection NN 147 149 by by IN 150 155 human human JJ 156 168 adenoviruses adenovirus NNS 169 171 is be VBZ 172 180 lymphoid lymphoid JJ 181 186 cells cell NNS 186 187 . . . 188 195 However however RB 195 196 , , , 197 205 analysis analysis NN 206 208 of of IN 209 212 the the DT 213 218 viral viral JJ 219 226 control control NN 227 235 elements element NNS 236 239 and and CC 240 243 the the DT 244 252 cellular cellular JJ 253 260 factors factor NNS 261 265 that that WDT 266 274 regulate regulate VBP 275 285 adenoviral adenoviral JJ 286 290 gene gene NN 291 301 expression expression NN 302 304 in in IN 305 316 lymphocytes lymphocyte NNS 317 320 has have VBZ 321 324 not not RB 325 329 been be VBN 330 338 reported report VBN 338 339 . . . 340 343 The the DT 344 354 adenovirus adenovirus NN 355 360 early early JJ 361 367 region region NN 368 369 3 3 CD 370 371 ( ( ( 371 373 ES ES NNP 373 374 ) ) ) 375 379 gene gene NN 380 388 products product NNS 389 392 are be VBP 393 401 involved involve VBN 402 404 in in IN 405 408 the the DT 409 420 maintenance maintenance NN 421 423 of of IN 424 429 viral viral JJ 430 441 persistence persistence NN 442 444 by by IN 445 455 complexing complexe VBG 456 460 with with IN 461 464 the the DT 465 470 class class NN 471 472 I i CD 473 476 MHC MHC NNP 477 485 antigens antigen NNS 485 486 , , , 487 491 thus thus RB 492 502 preventing prevent VBG 503 508 their their PRP$ 509 513 cell cell NN 514 521 surface surface NN 522 532 expression expression NN 533 537 with with IN 538 539 a a DT 540 549 resultant resultant JJ 550 558 decrease decrease NN 559 561 in in IN 562 566 host host NN 567 578 immunologic immunologic JJ 579 590 destruction destruction NN 590 591 . . . 592 594 To to TO 595 604 determine determine VB 605 612 whether whether IN 613 622 different different JJ 623 631 cellular cellular JJ 632 639 factors factor NNS 640 644 were be VBD 645 653 involved involve VBN 654 656 in in IN 657 659 E3 e3 NN 660 670 regulation regulation NN 671 673 in in IN 674 685 lymphocytes lymphocyte NNS 686 688 as as IN 689 697 compared compare VBN 698 702 with with IN 703 707 HeLa HeLa NNP 708 713 cells cell NNS 713 714 , , , 715 719 both both CC 720 723 DNA dna NN 724 731 binding binding NN 732 735 and and CC 736 748 transfection transfection NN 749 757 analysis analysis NN 758 762 with with IN 763 766 the the DT 767 769 E3 e3 NN 770 778 promoter promoter NN 779 781 in in IN 782 786 both both DT 787 791 cell cell NN 792 797 types type NNS 798 802 were be VBD 803 812 performed perform VBN 812 813 . . . 814 819 These these DT 820 827 studies study NNS 828 836 detected detect VBD 837 840 two two CD 841 846 novel novel JJ 847 854 domains domain NNS 855 863 referred refer VBN 864 866 to to TO 867 869 as as IN 870 872 L1 l1 NN 873 876 and and CC 877 879 L2 l2 NN 880 884 with with IN 885 886 a a DT 887 894 variety variety NN 895 897 of of IN 898 906 lymphoid lymphoid JJ 907 910 but but CC 911 914 not not RB 915 919 HeLa HeLa NNP 920 928 extracts extract NNS 928 929 . . . 930 934 Each each DT 935 937 of of IN 938 943 these these DT 944 951 domains domain NNS 952 961 possessed possess VBD 962 968 strong strong JJ 969 977 homology homology NN 978 980 to to TO 981 987 motifs motif NNS 988 998 previously previously RB 999 1004 found find VBN 1005 1007 to to TO 1008 1012 bind bind VB 1013 1016 the the DT 1017 1025 cellular cellular JJ 1026 1032 factor factor NN 1033 1041 NF-kappa NF-kappa NNP 1042 1044 B. B. NNP 1045 1058 Transfections transfection NNS 1059 1061 of of IN 1062 1064 E3 E3 NNP 1065 1075 constructs construct NNS 1076 1082 linked link VBN 1083 1085 to to TO 1086 1089 the the DT 1090 1105 chloramphenicol chloramphenicol NN 1106 1123 acetyltransferase acetyltransferase NN 1124 1128 gene gene NN 1129 1137 revealed reveal VBD 1138 1142 that that IN 1143 1154 mutagenesis mutagenesis NN 1155 1157 of of IN 1158 1161 the the DT 1162 1168 distal distal JJ 1169 1177 NF-kappa NF-kappa NNP 1178 1179 B B NNP 1180 1185 motif motif NN 1186 1187 ( ( ( 1187 1189 L2 L2 NNP 1189 1190 ) ) ) 1191 1194 had have VBD 1195 1202 minimal minimal JJ 1203 1210 effects effect NNS 1211 1213 on on IN 1214 1222 promoter promoter NN 1223 1233 expression expression NN 1234 1236 in in IN 1237 1241 HeLa HeLa NNP 1242 1247 cells cell NNS 1247 1248 , , , 1249 1252 but but CC 1253 1261 resulted result VBD 1262 1264 in in IN 1265 1273 dramatic dramatic JJ 1274 1283 decreases decrease NNS 1284 1286 in in IN 1287 1297 expression expression NN 1298 1300 by by IN 1301 1309 lymphoid lymphoid JJ 1310 1315 cells cell NNS 1315 1316 . . . 1317 1319 In in IN 1320 1328 contrast contrast NN 1328 1329 , , , 1330 1341 mutagenesis mutagenesis NN 1342 1344 of of IN 1345 1353 proximal proximal JJ 1354 1362 NF-kappa NF-kappa NNP 1363 1364 B B NNP 1365 1370 motif motif NN 1371 1372 ( ( ( 1372 1374 L1 L1 NNP 1374 1375 ) ) ) 1376 1379 had have VBD 1380 1387 minimal minimal JJ 1388 1395 effects effect NNS 1396 1398 on on IN 1399 1403 gene gene NN 1404 1414 expression expression NN 1415 1417 in in IN 1418 1422 both both DT 1423 1427 HeLa HeLa NNP 1428 1433 cells cell NNS 1434 1437 and and CC 1438 1446 lymphoid lymphoid JJ 1447 1452 cells cell NNS 1453 1456 but but CC 1457 1465 resulted result VBD 1466 1468 in in IN 1469 1470 a a DT 1471 1476 small small JJ 1476 1477 , , , 1478 1481 but but CC 1482 1494 reproducible reproducible JJ 1494 1495 , , , 1496 1504 increase increase NN 1505 1507 in in IN 1508 1512 gene gene NN 1513 1523 expression expression NN 1524 1526 in in IN 1527 1535 lymphoid lymphoid JJ 1536 1541 cells cell NNS 1542 1546 when when WRB 1547 1554 coupled couple VBN 1555 1557 to to TO 1558 1561 the the DT 1562 1564 L2 l2 NN 1565 1573 mutation mutation NN 1573 1574 . . . 1575 1584 Reversing reverse VBG 1585 1588 the the DT 1589 1597 position position NN 1598 1601 and and CC 1602 1612 subsequent subsequent JJ 1613 1624 mutagenesis mutagenesis NN 1625 1627 of of IN 1628 1631 the the DT 1632 1634 L1 l1 NN 1635 1638 and and CC 1639 1641 L2 l2 NN 1642 1649 domains domain NNS 1650 1659 indicated indicate VBD 1660 1664 that that IN 1665 1668 the the DT 1669 1676 primary primary JJ 1677 1685 sequence sequence NN 1686 1688 of of IN 1689 1694 these these DT 1695 1701 motifs motif NNS 1702 1708 rather rather RB 1709 1713 than than IN 1714 1719 their their PRP$ 1720 1728 position position NN 1729 1731 in in IN 1732 1735 the the DT 1736 1738 E3 e3 NN 1739 1747 promoter promoter NN 1748 1751 was be VBD 1752 1760 critical critical JJ 1761 1764 for for IN 1765 1775 regulating regulate VBG 1776 1780 gene gene NN 1781 1791 expression expression NN 1791 1792 . . . 1793 1794 ( ( ( 1794 1802 ABSTRACT abstract NN 1803 1812 TRUNCATED truncated VBN 1813 1815 AT at IN 1816 1819 250 250 CD 1820 1825 WORDS words NNS 1825 1826 ) ) )