0 10 Astrocytes Astrocyte NNP 11 14 and and CC 15 27 glioblastoma glioblastoma NN 28 33 cells cell NNS 34 41 express express VBP 42 47 novel novel JJ 48 59 octamer-DNA octamer-dna NN 60 67 binding binding NN 68 76 proteins protein NNS 77 85 distinct distinct JJ 86 90 from from IN 91 94 the the DT 95 105 ubiquitous ubiquitous JJ 106 111 Oct-1 oct-1 NN 112 115 and and CC 116 117 B B NNP 118 122 cell cell NN 123 127 type type NN 128 133 Oct-2 oct-2 NN 134 142 proteins protein NNS 142 143 . . . 145 148 The the DT 149 150 ‘ ` `` 150 157 octamer octamer NN 157 158 ’ ' '' 159 167 sequence sequence NN 167 168 , , , 169 177 ATGCAAAT ATGCAAAT NNP 178 180 or or CC 181 184 its its PRP$ 185 195 complement complement NN 196 204 ATTTGCAT ATTTGCAT NNP 204 205 , , , 206 208 is be VBZ 209 210 a a DT 211 214 key key JJ 215 222 element element NN 223 226 for for IN 227 230 the the DT 231 246 transcriptional transcriptional JJ 247 257 regulation regulation NN 258 260 of of IN 261 275 immunoglobulin immunoglobulin NN 276 281 genes gene NNS 282 284 in in IN 285 298 B-lymphocytes b-lymphocyte NNS 299 301 as as RB 302 306 well well RB 307 309 as as IN 310 311 a a DT 312 318 number number NN 319 321 of of IN 322 334 housekeeping housekeeping NN 335 340 genes gene NNS 341 343 in in IN 344 347 all all DT 348 352 cell cell NN 353 358 types type NNS 358 359 . . . 360 362 In in IN 363 374 lymphocytes lymphocyte NNS 374 375 , , , 376 379 the the DT 380 395 octamer-binding octamer-binding JJ 396 403 protein protein NN 404 410 Oct-2A Oct-2A NNP 411 414 and and CC 415 423 variants variant NNS 424 431 thereof thereof RB 432 435 are be VBP 436 443 thought think VBN 444 446 to to TO 447 457 contribute contribute VB 458 460 to to TO 461 464 the the DT 465 471 B-cell b-cell NN 472 480 specific specific JJ 481 485 gene gene NN 486 496 expression expression NN 496 497 , , , 498 503 while while IN 504 507 the the DT 508 518 ubiquitous ubiquitous JJ 519 526 protein protein NN 527 532 Oct-1 oct-1 NN 533 538 seems seem VBZ 539 541 to to TO 542 549 control control VB 550 557 general general JJ 558 565 octamer octamer NN 566 580 site-dependent site-dependent JJ 581 594 transcription transcription NN 594 595 . . . 596 603 Various various JJ 604 609 other other JJ 610 615 genes gene NNS 615 616 , , , 617 620 for for IN 621 628 example example NN 629 642 interleukin-1 interleukin-1 NN 643 646 and and CC 647 650 MHC MHC NNP 651 656 class class NN 657 659 II ii CD 660 665 genes gene NNS 665 666 , , , 667 674 contain contain VBP 675 677 an an DT 678 685 octamer octamer NN 686 694 sequence sequence NN 695 697 in in IN 698 701 the the DT 702 710 promoter promoter NN 711 714 and and CC 715 718 are be VBP 719 728 expressed express VBN 729 731 in in IN 732 737 cells cell NNS 738 740 of of IN 741 745 both both CC 746 749 the the DT 750 756 immune immune JJ 757 760 and and CC 761 768 nervous nervous JJ 769 776 systems system NNS 776 777 . . . 778 782 This this DT 783 791 prompted prompt VBD 792 794 us us PRP 795 797 to to TO 798 805 analyze analyze VB 806 809 the the DT 810 825 octamer-binding octamer-binding JJ 826 834 proteins protein NNS 835 837 in in IN 838 841 the the DT 842 848 latter latter JJ 849 854 cells cell NNS 854 855 . . . 856 861 Using use VBG 862 865 the the DT 866 881 electrophoretic electrophoretic JJ 882 890 mobility mobility NN 891 896 shift shift NN 897 902 assay assay NN 902 903 , , , 904 906 at at IN 907 912 least least JJS 913 916 six six CD 917 922 novel novel JJ 923 930 octamer octamer NN 931 938 binding binding NN 939 947 proteins protein NNS 948 952 were be VBD 953 961 detected detect VBN 962 964 in in IN 965 972 nuclear nuclear JJ 973 981 extracts extract NNS 982 984 of of IN 985 993 cultured culture VBN 994 999 mouse mouse NN 1000 1010 astrocytes astrocyte NNS 1010 1011 . . . 1012 1017 These these DT 1018 1026 proteins protein NNS 1027 1030 are be VBP 1031 1045 differentially differentially RB 1046 1055 expressed express VBN 1056 1058 in in IN 1059 1064 human human JJ 1065 1077 glioblastoma glioblastoma NN 1078 1081 and and CC 1082 1095 neuroblastoma neuroblastoma NN 1096 1100 cell cell NN 1101 1106 lines line NNS 1106 1107 . . . 1108 1111 The the DT 1112 1119 nervous nervous JJ 1120 1134 system-derived system-derived JJ 1135 1136 ( ( ( 1136 1141 N-Oct N-Oct NNP 1141 1142 ) ) ) 1143 1151 proteins protein NNS 1152 1157 bound bind VBN 1158 1160 to to TO 1161 1164 the the DT 1165 1172 octamer octamer NN 1173 1176 DNA dna NN 1177 1185 sequence sequence NN 1186 1188 in in IN 1189 1190 a a DT 1191 1197 manner manner NN 1198 1203 which which WDT 1204 1206 is be VBZ 1207 1224 indistinguishable indistinguishable JJ 1225 1229 from from IN 1230 1233 the the DT 1234 1239 Oct-1 Oct-1 NNP 1240 1243 and and CC 1244 1250 Oct-2A Oct-2A NNP 1251 1259 proteins protein NNS 1259 1260 . . . 1261 1264 The the DT 1265 1277 relationship relationship NN 1278 1280 of of IN 1281 1284 the the DT 1285 1290 N-Oct N-Oct NNP 1291 1299 proteins protein NNS 1300 1302 to to TO 1303 1308 Oct-1 Oct-1 NNP 1309 1312 and and CC 1313 1319 Oct-2A Oct-2A NNP 1320 1323 was be VBD 1324 1332 analyzed analyze VBN 1333 1335 by by IN 1336 1347 proteolytic proteolytic JJ 1348 1356 clipping clipping NN 1357 1366 bandshift bandshift NN 1367 1373 assays assay NNS 1374 1377 and and CC 1378 1380 by by IN 1381 1386 their their PRP$ 1387 1397 reactivity reactivity NN 1398 1405 towards towards IN 1406 1414 antisera antiserum NNS 1415 1421 raised raise VBN 1422 1429 against against IN 1430 1441 recombinant recombinant JJ 1442 1447 Oct-1 oct-1 NN 1448 1451 and and CC 1452 1458 Oct-2A Oct-2A NNP 1459 1467 proteins protein NNS 1467 1468 . . . 1469 1471 On on IN 1472 1475 the the DT 1476 1481 basis basis NN 1482 1484 of of IN 1485 1490 these these DT 1491 1497 assays assay NNS 1497 1498 , , , 1499 1502 all all DT 1503 1516 N-Oct-factors n-oct-factor NNS 1517 1521 were be VBD 1522 1527 found find VBN 1528 1530 to to TO 1531 1533 be be VB 1534 1542 distinct distinct JJ 1543 1547 from from IN 1548 1551 the the DT 1552 1562 ubiquitous ubiquitous JJ 1563 1568 Oct-1 oct-1 NN 1569 1572 and and CC 1573 1576 the the DT 1577 1594 lymphoid-specific lymphoid-specific JJ 1595 1601 Oct-2A Oct-2A NNP 1602 1610 proteins protein NNS 1610 1611 . . . 1612 1614 In in IN 1615 1623 melanoma melanoma NN 1624 1629 cells cell NNS 1630 1634 that that WDT 1635 1642 contain contain VBP 1643 1646 the the DT 1647 1654 N-Oct-3 n-oct-3 NN 1655 1661 factor factor NN 1661 1662 , , , 1663 1664 a a DT 1665 1676 transfected transfecte VBN 1677 1696 lymphocyte-specific lymphocyte-specific JJ 1697 1705 promoter promoter NN 1706 1709 was be VBD 1710 1717 neither neither CC 1718 1727 activated activate VBD 1728 1731 nor nor CC 1732 1735 was be VBD 1736 1738 it it PRP 1739 1748 repressed repress VBD 1749 1753 upon upon IN 1754 1769 contransfection contransfection NN 1770 1774 with with IN 1775 1777 an an DT 1778 1784 Oct-2A Oct-2A NNP 1785 1795 expression expression NN 1796 1802 vector vector NN 1802 1803 . . . 1804 1806 We we PRP 1807 1816 therefore therefore RB 1817 1826 speculate speculate VBP 1827 1831 that that IN 1832 1839 N-Oct-3 n-oct-3 NN 1840 1843 and and CC 1844 1849 other other JJ 1850 1855 N-Oct N-Oct NNP 1856 1863 factors factor NNS 1864 1868 have have VBP 1869 1870 a a DT 1871 1879 specific specific JJ 1880 1884 role role NN 1885 1887 in in IN 1888 1892 gene gene NN 1893 1903 expression expression NN 1904 1906 in in IN 1907 1912 cells cell NNS 1913 1915 of of IN 1916 1919 the the DT 1920 1927 nervous nervous JJ 1928 1934 system system NN 1934 1935 . . .