0 2 An an DT 3 5 in in FW 6 11 vitro vitro FW 12 18 globin globin NN 19 23 gene gene NN 24 33 switching switching NN 34 39 model model NN 40 45 based base VBN 46 48 on on IN 49 63 differentiated differentiate VBN 64 73 embryonic embryonic JJ 74 78 stem stem NN 79 84 cells cell NNS 84 85 . . . 87 89 We we PRP 90 94 used use VBD 95 100 mouse mouse NN 101 110 embryonic embryonic JJ 111 115 stem stem NN 116 117 ( ( ( 117 119 ES ES NNP 119 120 ) ) ) 121 126 cells cell NNS 127 129 to to TO 130 135 study study VB 136 142 globin globin NN 143 147 gene gene NN 148 158 expression expression NN 159 162 and and CC 163 172 switching switching NN 173 175 in in FW 176 181 vitro vitro FW 181 182 . . . 183 185 We we PRP 186 190 show show VBP 191 195 that that IN 196 206 ES-derived es-derived JJ 207 215 embryoid embryoid JJ 216 222 bodies body NNS 223 230 express express VBP 231 234 the the DT 235 239 full full JJ 240 250 complement complement NN 251 253 of of IN 254 259 mouse mouse NN 260 269 embryonic embryonic JJ 270 276 globin globin NN 277 282 genes gene NNS 283 285 in in IN 286 289 the the DT 290 297 correct correct JJ 298 306 temporal temporal JJ 307 312 order order NN 313 316 and and CC 317 321 that that IN 322 324 on on IN 325 332 further further JJ 333 348 differentiation differentiation NN 348 349 , , , 350 351 a a DT 352 358 switch switch NN 359 365 occurs occur VBZ 366 368 to to TO 369 372 the the DT 373 384 fetal/adult fetal/adult JJ 385 390 genes gene NNS 390 391 . . . 392 394 In in IN 395 403 addition addition NN 403 404 , , , 405 408 the the DT 409 427 erythroid-specific erythroid-specific JJ 428 441 transcription transcription NN 442 448 factor factor NN 449 454 NF-E1 nf-e1 NN 455 458 was be VBD 459 464 shown show VBN 465 467 to to TO 468 470 be be VB 471 480 expressed express VBN 481 493 coordinately coordinately RB 494 498 with with IN 499 503 that that DT 504 506 of of IN 507 513 globin globin NN 514 516 in in IN 517 525 embryoid embryoid JJ 526 532 bodies body NNS 532 533 . . . 534 536 We we PRP 537 545 conclude conclude VBP 546 550 from from IN 551 556 these these DT 557 568 experiments experiment NNS 569 573 that that IN 574 577 the the DT 578 580 ES ES NNP 581 585 cell cell NN 586 592 system system NN 593 601 provides provide VBZ 602 603 a a DT 604 608 good good JJ 609 614 model model NN 615 617 to to TO 618 623 study study VB 624 637 hematopoietic hematopoietic JJ 638 649 development development NN 649 650 . . . 651 655 When when WRB 656 659 the the DT 660 665 human human JJ 666 674 epsilon- epsilon- NN 675 677 or or CC 678 689 beta-globin beta-globin NN 690 695 genes gene NNS 696 702 driven drive VBN 703 705 by by IN 706 709 the the DT 710 718 dominant dominant JJ 719 726 control control NN 727 733 region region NN 734 735 ( ( ( 735 738 DCR DCR NNP 738 739 ) ) ) 740 743 are be VBP 744 754 introduced introduce VBN 755 759 into into IN 760 764 this this DT 765 771 system system NN 771 772 , , , 773 776 the the DT 777 782 human human JJ 783 797 epsilon-globin epsilon-globin NN 798 802 gene gene NN 802 803 , , , 804 806 in in IN 807 815 contrast contrast NN 816 818 to to TO 819 822 the the DT 823 834 beta-globin beta-globin NN 835 839 gene gene NN 839 840 , , , 841 843 is be VBZ 844 847 not not RB 848 859 deregulated deregulate VBN 860 862 by by IN 863 866 the the DT 867 875 presence presence NN 876 878 of of IN 879 882 the the DT 883 886 DCR DCR NNP 887 890 and and CC 891 893 is be VBZ 894 903 expressed express VBN 904 912 strictly strictly RB 913 915 as as IN 916 918 an an DT 919 928 embryonic embryonic JJ 929 933 gene gene NN 933 934 . . . 935 937 We we PRP 938 946 conclude conclude VBP 947 951 from from IN 952 956 this this DT 957 961 that that IN 962 965 the the DT 966 980 epsilon-globin epsilon-globin NN 981 985 gene gene NN 986 988 is be VBZ 989 992 not not RB 993 1002 regulated regulate VBN 1003 1005 by by IN 1006 1017 competition competition NN 1018 1022 with with IN 1023 1028 other other JJ 1029 1034 genes gene NNS 1035 1037 in in IN 1038 1041 the the DT 1042 1047 human human JJ 1048 1059 beta-globin beta-globin NN 1060 1065 locus locus NN 1065 1066 . . .