0 13 Cross-linking cross-linking JJ 14 18 CD40 CD40 NNP 19 21 on on IN 22 23 B B NNP 24 29 cells cell NNS 30 37 rapidly rapidly RB 38 47 activates activate VBZ 48 55 nuclear nuclear JJ 56 68 factor-kappa factor-kappa NN 69 71 B. B. NNP 73 76 The the DT 77 78 B B NNP 79 94 cell-associated cell-associated JJ 95 102 surface surface NN 103 111 molecule molecule NN 112 116 CD40 cd40 NN 117 126 functions function VBZ 127 129 to to TO 130 138 regulate regulate VB 139 140 B B NNP 141 145 cell cell NN 146 155 responses response NNS 155 156 . . . 157 170 Cross-linking cross-linking JJ 171 175 CD40 CD40 NNP 176 178 on on IN 179 180 B B NNP 181 186 cells cell NNS 187 190 can can MD 191 195 lead lead VB 196 198 to to TO 199 208 homotypic homotypic JJ 209 213 cell cell NN 214 222 adhesion adhesion NN 222 223 , , , 224 228 IL-6 IL-6 NNP 229 239 production production NN 239 240 , , , 241 244 and and CC 244 245 , , , 246 248 in in IN 249 260 combination combination NN 261 265 with with IN 266 275 cytokines cytokine NNS 275 276 , , , 277 279 to to TO 280 282 Ig Ig NNP 283 290 isotype isotype NN 291 300 switching switching NN 300 301 . . . 302 310 Tyrosine Tyrosine NNP 311 317 kinase kinase NNP 318 326 activity activity NN 327 329 is be VBZ 330 339 increased increase VBN 340 347 shortly shortly RB 348 353 after after IN 354 364 engagement engagement NN 365 367 of of IN 368 372 this this DT 373 381 receptor receptor NN 381 382 . . . 383 389 Little little JJ 390 392 is be VBZ 393 398 known know VBN 399 404 about about IN 405 408 how how WRB 409 412 the the DT 413 417 very very RB 418 423 early early JJ 424 430 events event NNS 431 438 induced induce VBN 439 441 by by IN 442 446 CD40 CD40 NNP 447 460 cross-linking cross-linking NN 461 465 link link VBP 466 468 to to TO 469 477 cellular cellular JJ 478 487 responses response NNS 487 488 . . . 489 491 In in IN 492 496 this this DT 497 502 study study NN 502 503 , , , 504 506 we we PRP 507 518 demonstrate demonstrate VBP 519 523 that that IN 524 531 nuclear nuclear JJ 532 538 factor factor NN 539 540 ( ( ( 540 549 NF)-kappa NF)-kappa NNP 550 551 B B NNP 552 555 and and CC 556 564 NF-kappa NF-kappa NNP 565 571 B-like b-like JJ 572 585 transcription transcription NN 586 593 factors factor NNS 594 597 are be VBP 598 607 activated activate VBN 608 613 after after IN 614 627 cross-linking cross-linking JJ 628 632 CD40 cd40 NN 633 635 on on IN 636 643 resting rest VBG 644 649 human human JJ 650 659 tonsillar tonsillar JJ 660 661 B b NN 662 667 cells cell NNS 668 671 and and CC 672 674 on on IN 675 676 B B NNP 677 681 cell cell NN 682 687 lines line NNS 687 688 . . . 689 692 The the DT 693 703 activation activation NN 704 706 is be VBZ 707 712 rapid rapid JJ 713 716 and and CC 717 719 is be VBZ 720 728 mediated mediate VBN 729 736 through through IN 737 738 a a DT 739 747 tyrosine tyrosine NN 748 764 kinase-dependent kinase-dependent JJ 765 772 pathway pathway NN 772 773 . . . 774 777 The the DT 778 787 complexes complex NNS 788 796 detected detect VBN 797 799 in in IN 800 815 electrophoretic electrophoretic JJ 816 824 mobility mobility NN 825 830 shift shift NN 831 837 assays assay NNS 838 845 contain contain VBP 846 849 p50 p50 NN 849 850 , , , 851 854 p65 p65 NN 855 856 ( ( ( 856 860 RelA RelA NNP 860 861 ) ) ) 861 862 , , , 863 868 c-Rel c-rel NN 868 869 , , , 870 873 and and CC 874 878 most most RBS 879 885 likely likely JJ 886 891 other other JJ 892 902 components component NNS 902 903 . . . 904 906 By by IN 907 912 using use VBG 913 922 transient transient JJ 923 935 transfection transfection NN 936 942 assays assay NNS 942 943 , , , 944 946 we we PRP 947 952 found find VBD 953 957 that that IN 958 971 cross-linking cross-linking JJ 972 976 CD40 cd40 NN 977 985 supports support VBZ 986 994 NF-kappa NF-kappa NNP 995 1006 B-dependent b-dependent JJ 1007 1011 gene gene NN 1012 1022 expression expression NN 1022 1023 . . . 1024 1027 Our our PRP$ 1028 1035 results result NNS 1036 1042 define define VBP 1043 1046 the the DT 1047 1055 NF-kappa NF-kappa NNP 1056 1057 B B NNP 1058 1064 system system NN 1065 1067 as as IN 1068 1070 an an DT 1071 1083 intermediate intermediate JJ 1084 1089 event event NN 1090 1092 in in IN 1093 1097 CD40 CD40 NNP 1098 1107 signaling signaling NN 1108 1111 and and CC 1112 1119 suggest suggest VBP 1120 1124 that that IN 1125 1128 the the DT 1129 1133 CD40 cd40 NN 1134 1141 pathway pathway NN 1142 1145 can can MD 1146 1155 influence influence VB 1156 1159 the the DT 1160 1170 expression expression NN 1171 1173 of of IN 1174 1175 B B NNP 1176 1191 cell-associated cell-associated JJ 1192 1197 genes gene NNS 1198 1202 with with IN 1203 1211 NF-kappa NF-kappa NNP 1212 1213 B B NNP 1214 1223 consensus consensus NN 1224 1229 sites site NNS 1229 1230 . . .