0 26 Lipopolysaccharide-induced lipopolysaccharide-induced JJ 27 37 E-selectin E-selectin NNP 38 48 expression expression NN 49 57 requires require VBZ 58 68 continuous continuous JJ 69 77 presence presence NN 78 80 of of IN 81 84 LPS LPS NNP 85 88 and and CC 89 91 is be VBZ 92 101 inhibited inhibit VBN 102 104 by by IN 105 141 bactericidal/permeability-increasing bactericidal/permeability-increasing JJ 142 149 protein protein NN 149 150 . . . 152 163 Endothelial endothelial JJ 164 169 cells cell NNS 170 180 stimulated stimulate VBN 181 183 by by IN 184 187 LPS lps NN 188 195 express express VBP 196 206 E-selectin E-selectin NNP 206 207 , , , 208 213 which which WDT 214 219 plays play VBZ 220 222 an an DT 223 232 important important JJ 233 237 role role NN 238 240 in in IN 241 250 mediating mediate VBG 251 261 neutrophil neutrophil NN 262 270 adhesion adhesion NN 271 277 during during IN 278 290 inflammation inflammation NN 290 291 . . . 292 302 E-selectin E-selectin NNP 303 305 is be VBZ 306 313 induced induce VBN 314 320 within within IN 321 324 1-2 1-2 CD 325 326 h h NN 326 327 , , , 328 333 peaks peak VBZ 334 336 at at IN 337 340 4-6 4-6 CD 341 342 h h NN 342 343 , , , 344 347 and and CC 348 357 gradually gradually RB 358 365 returns return VBZ 366 368 to to TO 369 374 basal basal JJ 375 380 level level NN 381 383 by by IN 384 386 24 24 CD 387 389 h. h. NN 390 396 rBPI21 rbpi21 NN 396 397 , , , 398 399 a a DT 400 411 recombinant recombinant JJ 412 422 N-terminal n-terminal JJ 423 431 fragment fragment NN 432 434 of of IN 435 440 human human JJ 441 477 bactericidal/permeability-increasing bactericidal/permeability-increasing JJ 478 485 protein protein NN 486 487 ( ( ( 487 490 BPI BPI NNP 490 491 ) ) ) 491 492 , , , 493 502 inhibited inhibit VBD 503 514 LPS-induced lps-induced JJ 515 525 E-selectin E-selectin NNP 526 536 expression expression NN 537 541 when when WRB 542 547 added add VBN 548 550 at at IN 551 554 the the DT 555 559 same same JJ 560 564 time time NN 565 567 as as IN 567 568 , , , 569 572 and and CC 573 575 up up RB 576 578 to to TO 579 580 6 6 CD 581 582 h h NN 583 588 after after IN 588 589 , , , 590 593 LPS LPS NNP 593 594 . . . 595 602 Delayed delay VBN 603 617 administration administration NN 618 620 of of IN 621 627 rBPI21 rbpi21 NN 628 632 also also RB 633 641 affected affect VBD 642 654 LPS-mediated lps-mediated JJ 655 665 activation activation NN 666 668 of of IN 669 672 the the DT 673 680 nuclear nuclear JJ 681 687 factor factor NN 687 688 , , , 689 697 NF-kappa NF-kappa NNP 698 700 B. B. NNP 701 704 Two two CD 705 707 to to TO 708 709 4 4 CD 710 711 h h NN 712 721 following follow VBG 722 725 LPS LPS NNP 726 734 addition addition NN 735 737 to to TO 738 749 endothelial endothelial JJ 750 755 cells cell NNS 755 756 , , , 757 761 when when WRB 762 770 NF-kappa NF-kappa NNP 771 772 B B NNP 773 776 was be VBD 777 784 already already RB 785 794 activated activate VBN 794 795 , , , 796 804 addition addition NN 805 807 of of IN 808 814 rBPI21 rbpi21 NN 815 823 resulted result VBD 824 826 in in IN 827 833 marked marked JJ 834 843 reduction reduction NN 844 846 of of IN 847 855 NF-kappa NF-kappa NNP 856 857 B B NNP 858 868 detectable detectable JJ 869 871 at at IN 872 873 4 4 CD 874 876 or or CC 877 878 6 6 CD 879 880 h h NN 880 881 . . . 882 887 These these DT 888 895 results result NNS 896 904 indicate indicate VBP 905 909 that that IN 910 921 endothelial endothelial JJ 922 932 activation activation NN 933 941 requires require VBZ 942 952 continuous continuous JJ 953 961 presence presence NN 962 964 of of IN 965 968 LPS LPS NNP 968 969 , , , 970 973 and and CC 974 980 rBPI21 rbpi21 NN 981 985 acts act VBZ 986 988 to to TO 989 996 reverse reverse VB 997 1009 LPS-mediated lps-mediated JJ 1010 1021 endothelial endothelial JJ 1022 1032 activation activation NN 1033 1035 by by IN 1036 1048 interrupting interrupt VBG 1049 1052 the the DT 1053 1061 on-going on-going JJ 1062 1065 LPS lps NN 1066 1072 signal signal NN 1072 1073 . . .