0 13 Costimulation costimulation NN 14 16 of of IN 17 22 human human JJ 23 27 CD4+ cd4+ JJ 28 29 T t NN 30 35 cells cell NNS 36 40 with with IN 41 46 LFA-3 LFA-3 NNP 47 50 and and CC 51 53 B7 B7 NNP 54 60 induce induce VBP 61 69 distinct distinct JJ 70 77 effects effect NNS 78 80 on on IN 81 85 AP-1 AP-1 NNP 86 89 and and CC 90 98 NF-kappa NF-kappa NNP 99 100 B B NNP 101 114 transcription transcription NN 115 122 factors factor NNS 122 123 . . . 125 127 We we PRP 128 132 have have VBP 133 140 earlier earlier RBR 141 146 shown show VBN 147 151 that that IN 152 163 stimulation stimulation NN 164 166 of of IN 167 172 human human JJ 173 177 CD4+ cd4+ JJ 178 179 T t NN 180 185 cells cell NNS 186 190 with with IN 191 194 SEA SEA NNP 195 204 presented present VBN 205 207 on on IN 208 215 Chinese chinese JJ 216 223 hamster hamster NN 224 229 ovary ovary NN 230 231 ( ( ( 231 238 CHO)-DR CHO)-DR NNP 239 252 transfectants transfectant NNS 253 265 coexpressing coexpresse VBG 266 272 either either CC 273 275 B7 B7 NNP 276 278 or or CC 279 284 LFA-3 LFA-3 NNP 285 293 resulted result VBD 294 296 in in IN 297 305 distinct distinct JJ 306 314 cytokine cytokine NN 315 323 profiles profile NNS 323 324 . . . 325 327 We we PRP 328 331 now now RB 332 343 demonstrate demonstrate VBP 344 348 that that IN 349 351 B7 b7 NN 351 352 , , , 353 356 but but CC 357 360 not not RB 361 366 LFA-3 lfa-3 NN 366 367 , , , 368 376 strongly strongly RB 377 389 costimulated costimulate VBD 390 394 IL-2 il-2 NN 395 408 transcription transcription NN 409 412 and and CC 413 417 mRNA mrna NN 418 428 expression expression NN 429 431 in in IN 432 436 CD4+ cd4+ JJ 437 438 T t NN 439 444 cells cell NNS 444 445 . . . 446 453 Maximal maximal JJ 454 462 increase increase NN 463 465 in in IN 466 470 IL-2 il-2 NN 471 484 transcription transcription NN 485 488 was be VBD 489 497 recorded record VBN 498 502 with with IN 503 518 CHO-DR/B7/LFA-3 CHO-DR/B7/LFA-3 NNP 518 519 , , , 520 530 suggesting suggest VBG 531 532 a a DT 533 544 cooperative cooperative JJ 545 551 effect effect NN 552 554 of of IN 555 557 B7 B7 NNP 558 561 and and CC 562 567 LFA-3 LFA-3 NNP 568 570 at at IN 571 574 the the DT 575 590 transcriptional transcriptional JJ 591 596 level level NN 596 597 . . . 598 607 Gel-shift gel-shift JJ 608 616 analysis analysis NN 617 629 demonstrated demonstrate VBD 630 634 that that IN 635 646 stimulation stimulation NN 647 649 of of IN 650 654 CD4+ cd4+ JJ 655 656 T t NN 657 662 cells cell NNS 663 667 with with IN 668 674 CHO-DR CHO-DR NNP 675 678 and and CC 679 693 staphylococcal staphylococcal JJ 694 705 enterotoxin enterotoxin NN 706 707 A a NN 708 711 was be VBD 712 722 sufficient sufficient JJ 723 725 to to TO 726 732 induce induce VB 733 744 significant significant JJ 745 752 amounts amount NNS 753 755 of of IN 756 764 NF-kappa NF-kappa NNP 765 766 B B NNP 767 774 binding binding NN 775 783 proteins protein NNS 783 784 , , , 785 792 whereas whereas IN 793 802 induction induction NN 803 805 of of IN 806 810 AP-1 ap-1 NN 811 818 binding binding NN 819 827 proteins protein NNS 828 836 required require VBD 837 850 costimulation costimulation NN 850 851 . . . 852 857 LFA-3 LFA-3 NNP 858 865 induced induce VBD 866 874 moderate moderate JJ 875 881 levels level NNS 882 884 of of IN 885 889 AP-1 ap-1 NN 889 890 , , , 891 894 but but CC 895 898 did do VBD 899 902 not not RB 903 912 influence influence VB 913 916 the the DT 917 923 levels level NNS 924 926 of of IN 927 935 NF-kappa NF-kappa NNP 936 937 B B NNP 937 938 , , , 939 944 while while IN 945 947 B7 b7 NN 948 961 costimulation costimulation NN 962 970 strongly strongly RB 971 978 induced induce VBD 979 983 both both CC 984 988 AP-1 ap-1 NN 989 992 and and CC 993 1006 substantially substantially RB 1007 1015 enhanced enhance VBN 1016 1024 NF-kappa NF-kappa NNP 1025 1026 B B NNP 1027 1034 binding binding NN 1035 1043 proteins protein NNS 1043 1044 . . . 1045 1048 The the DT 1049 1064 CHO-DR/B7/LFA-3 cho-dr/b7/lfa-3 NN 1065 1071 triple triple JJ 1072 1084 transfectant transfectant NN 1085 1092 induced induce VBD 1093 1094 a a DT 1095 1102 further further JJ 1103 1111 increase increase NN 1112 1114 in in IN 1115 1119 AP-1 AP-1 NNP 1120 1123 and and CC 1124 1132 NF-kappa NF-kappa NNP 1133 1134 B B NNP 1135 1142 binding binding NN 1143 1151 proteins protein NNS 1152 1160 compared compare VBN 1161 1165 with with IN 1166 1169 the the DT 1170 1176 double double JJ 1177 1190 transfectants transfectant NNS 1190 1191 . . . 1192 1195 The the DT 1196 1201 level level NN 1202 1204 of of IN 1205 1210 Oct-1 oct-1 NN 1211 1218 binding binding NN 1219 1227 proteins protein NNS 1228 1236 remained remain VBD 1237 1244 similar similar JJ 1245 1247 in in IN 1248 1251 all all DT 1252 1259 samples sample NNS 1259 1260 . . . 1261 1272 Super-shift super-shift JJ 1273 1281 analysis analysis NN 1282 1290 revealed reveal VBD 1291 1295 that that IN 1296 1299 the the DT 1300 1308 NF-kappa NF-kappa NNP 1309 1310 B B NNP 1311 1318 complex complex NN 1319 1321 of of IN 1322 1334 costimulated costimulate VBN 1335 1339 CD4+ cd4+ JJ 1340 1341 T t NN 1342 1347 cells cell NNS 1348 1357 contained contain VBD 1358 1363 large large JJ 1364 1371 amounts amount NNS 1372 1374 of of IN 1375 1378 p50 p50 NN 1378 1379 , , , 1380 1391 substantial substantial JJ 1392 1399 amounts amount NNS 1400 1402 of of IN 1403 1406 p65 p65 NN 1406 1407 , , , 1408 1411 and and CC 1412 1420 marginal marginal JJ 1421 1427 levels level NNS 1428 1430 of of IN 1431 1436 c-Rel c-rel NN 1437 1445 proteins protein NNS 1445 1446 . . . 1447 1450 The the DT 1451 1455 AP-1 ap-1 NN 1456 1463 binding binding NN 1464 1472 proteins protein NNS 1473 1482 contained contain VBD 1483 1488 c-Jun c-jun NN 1488 1489 , , , 1490 1495 Jun-D Jun-D NNP 1495 1496 , , , 1497 1500 and and CC 1501 1506 Fra-1 fra-1 NN 1506 1507 , , , 1508 1511 but but CC 1512 1520 marginal marginal JJ 1521 1528 amounts amount NNS 1529 1531 of of IN 1532 1537 Jun-B Jun-B NNP 1538 1541 and and CC 1542 1547 c-Fos c-fos NN 1547 1548 . . . 1549 1552 Our our PRP$ 1553 1560 results result NNS 1561 1569 indicate indicate VBP 1570 1578 distinct distinct JJ 1579 1586 effects effect NNS 1587 1589 of of IN 1590 1592 B7 B7 NNP 1593 1596 and and CC 1597 1602 LFA-3 lfa-3 NN 1603 1616 costimulation costimulation NN 1617 1619 on on IN 1620 1623 the the DT 1624 1632 activity activity NN 1633 1635 of of IN 1636 1640 AP-1 AP-1 NNP 1641 1644 and and CC 1645 1653 NF-kappa NF-kappa NNP 1654 1656 B. B. NNP 1657 1662 These these DT 1663 1666 may may MD 1667 1673 partly partly RB 1674 1681 account account VB 1682 1685 for for IN 1686 1689 the the DT 1690 1702 differential differential JJ 1703 1710 effects effect NNS 1711 1713 of of IN 1714 1716 B7 B7 NNP 1717 1720 and and CC 1721 1726 LFA-3 lfa-3 NN 1727 1740 costimulation costimulation NN 1741 1743 on on IN 1744 1748 IL-2 il-2 NN 1749 1759 expression expression NN 1759 1760 . . .