0 10 Regulation regulation NN 11 13 of of IN 14 36 granulocyte-macrophage granulocyte-macrophage JJ 37 55 colony-stimulating colony-stimulating JJ 56 62 factor factor NN 63 66 and and CC 67 77 E-selectin E-selectin NNP 78 88 expression expression NN 89 91 in in IN 92 103 endothelial endothelial JJ 104 109 cells cell NNS 110 112 by by IN 113 124 cyclosporin cyclosporin NN 125 126 A a NN 127 130 and and CC 131 134 the the DT 135 141 T-cell t-cell NN 142 155 transcription transcription NN 156 162 factor factor NN 163 167 NFAT nfat NN 167 168 . . . 170 177 Nuclear nuclear JJ 178 184 factor factor NN 185 187 of of IN 188 197 activated activate VBN 198 199 T t NN 200 205 cells cell NNS 206 207 ( ( ( 207 211 NFAT NFAT NNP 211 212 ) ) ) 213 216 was be VBD 217 227 originally originally RB 228 237 described describe VBN 238 240 as as IN 241 242 a a DT 243 258 T-cell-specific t-cell-specific JJ 259 272 transcription transcription NN 273 279 factor factor NN 280 285 athat athat WDT 286 295 supported support VBD 296 299 the the DT 300 310 activation activation NN 311 313 of of IN 314 322 cytokine cytokine NN 323 327 gene gene NN 328 338 expression expression NN 339 342 and and CC 343 351 mediated mediate VBD 352 355 the the DT 356 372 immunoregulatory immunoregulatory JJ 373 380 effects effect NNS 381 383 of of IN 384 395 cyclosporin cyclosporin NN 396 397 A A NNP 398 399 ( ( ( 399 402 CsA CsA NNP 402 403 ) ) ) 403 404 . . . 405 407 As as IN 408 410 we we PRP 411 419 observed observe VBD 420 424 that that IN 425 434 activated activate VBN 435 446 endothelial endothelial JJ 447 452 cells cell NNS 453 457 also also RB 458 467 expressed express VBD 468 472 NFAT NFAT NNP 472 473 , , , 474 476 we we PRP 477 483 tested test VBD 484 487 the the DT 488 504 antiinflammatory antiinflammatory JJ 505 515 properties property NNS 516 518 of of IN 519 522 CsA CsA NNP 523 525 in in IN 526 537 endothelial endothelial JJ 538 543 cells cell NNS 543 544 . . . 545 558 Significantly significantly RB 558 559 , , , 560 563 CsA CsA NNP 564 574 completely completely RB 575 585 suppressed suppress VBD 586 589 the the DT 590 599 induction induction NN 600 602 of of IN 603 607 NFAT NFAT NNP 608 610 in in IN 611 622 endothelial endothelial JJ 623 628 cells cell NNS 629 632 and and CC 633 642 inhibited inhibit VBD 643 646 the the DT 647 655 activity activity NN 656 658 of of IN 659 681 granulocyte-macrophage granulocyte-macrophage JJ 682 700 colony-stimulating colony-stimulating JJ 701 707 factor factor NN 708 709 ( ( ( 709 715 GM-CSF GM-CSF NNP 715 716 ) ) ) 717 721 gene gene NN 722 732 regulatory regulatory JJ 733 741 elements element NNS 742 746 that that WDT 747 750 use use VBP 751 755 NFAT nfat NN 756 758 by by IN 759 761 60 60 CD 761 762 % % NN 762 763 . . . 764 767 CsA CsA NNP 768 777 similarly similarly RB 778 786 mediated mediate VBD 787 788 a a DT 789 798 reduction reduction NN 799 801 of of IN 802 804 up up IN 805 807 to to TO 808 810 65 65 CD 810 811 % % NN 812 814 in in IN 815 821 GM-CSF GM-CSF NNP 822 826 mRNA mRNA NNP 827 830 and and CC 831 838 protein protein NN 839 849 expression expression NN 850 852 in in IN 853 862 activated activate VBN 863 874 endothelial endothelial JJ 875 880 cells cell NNS 880 881 . . . 882 885 CsA CsA NNP 886 890 also also RB 891 901 suppressed suppress VBD 902 912 E-selectin E-selectin NNP 912 913 , , , 914 917 but but CC 918 921 not not RB 922 930 vascular vascular JJ 931 935 cell cell NN 936 944 adhesion adhesion NN 945 955 molecule-1 molecule-1 NN 956 957 ( ( ( 957 963 VCAM-1 vcam-1 NN 963 964 ) ) ) 965 975 expression expression NN 976 978 in in IN 979 990 endothelial endothelial JJ 991 996 cells cell NNS 996 997 , , , 998 1002 even even RB 1003 1009 though though IN 1010 1013 the the DT 1014 1024 E-selectin E-selectin NNP 1025 1033 promoter promoter NN 1034 1036 is be VBZ 1037 1046 activated activate VBN 1047 1049 by by IN 1050 1058 NF-kappa NF-kappa NNP 1059 1060 B B NNP 1061 1067 rather rather RB 1068 1072 than than IN 1073 1077 NFAT NFAT NNP 1077 1078 . . . 1079 1084 Hence hence RB 1084 1085 , , , 1086 1095 induction induction NN 1096 1098 of of IN 1099 1103 cell cell NN 1104 1111 surface surface NN 1112 1122 expression expression NN 1123 1125 of of IN 1126 1130 this this DT 1131 1140 leukocyte leukocyte NN 1141 1149 adhesion adhesion NN 1150 1158 molecule molecule NN 1159 1161 by by IN 1162 1167 tumor tumor NN 1168 1176 necrosis necrosis NN 1177 1183 factor factor NN 1184 1185 ( ( ( 1185 1195 TNF)-alpha TNF)-alpha NNP 1196 1199 was be VBD 1200 1207 reduced reduce VBN 1208 1210 by by IN 1211 1213 40 40 CD 1213 1214 % % NN 1215 1217 in in IN 1218 1221 the the DT 1222 1230 presence presence NN 1231 1233 of of IN 1234 1237 CsA CsA NNP 1237 1238 , , , 1239 1242 and and CC 1243 1247 this this DT 1248 1251 was be VBD 1252 1261 reflected reflect VBN 1262 1264 by by IN 1265 1266 a a DT 1267 1269 29 29 CD 1269 1270 % % NN 1271 1279 decrease decrease NN 1280 1282 in in IN 1283 1293 neutrophil neutrophil NN 1294 1302 adhesion adhesion NN 1302 1303 . . . 1304 1307 The the DT 1308 1315 effects effect NNS 1316 1318 of of IN 1319 1322 CsA CsA NNP 1323 1325 on on IN 1326 1337 endothelial endothelial JJ 1338 1343 cells cell NNS 1344 1348 were be VBD 1349 1353 also also RB 1354 1362 detected detect VBN 1363 1365 at at IN 1366 1369 the the DT 1370 1379 chromatin chromatin NN 1380 1389 structure structure NN 1390 1395 level level NN 1395 1396 , , , 1397 1399 as as IN 1400 1406 DNasel DNasel NNP 1407 1421 hypersensitive hypersensitive JJ 1422 1427 sites site NNS 1428 1434 within within IN 1435 1439 both both CC 1440 1443 the the DT 1444 1450 GM-CSF GM-CSF NNP 1451 1459 enhancer enhancer NN 1460 1463 and and CC 1464 1467 the the DT 1468 1478 E-selectin E-selectin NNP 1479 1487 promoter promoter NN 1488 1492 were be VBD 1493 1503 suppressed suppress VBN 1504 1506 by by IN 1507 1510 CsA CsA NNP 1510 1511 . . . 1512 1516 This this DT 1517 1527 represents represent VBZ 1528 1531 the the DT 1532 1537 first first JJ 1538 1544 report report NN 1545 1547 of of IN 1548 1552 NFAT NFAT NNP 1553 1555 in in IN 1556 1567 endothelial endothelial JJ 1568 1573 cells cell NNS 1574 1577 and and CC 1578 1586 suggests suggest VBZ 1587 1597 mechanisms mechanism NNS 1598 1600 by by IN 1601 1606 which which WDT 1607 1610 CsA CsA NNP 1611 1616 could could MD 1617 1625 function function VB 1626 1628 as as IN 1629 1631 an an DT 1632 1648 antiinflammatory antiinflammatory JJ 1649 1654 agent agent NN 1654 1655 . . .