0	15	Transcriptional	transcriptional	JJ
16	26	repression	repression	NN
27	29	of	of	IN
30	33	the	the	DT
34	47	interleukin-2	interleukin-2	NN
48	52	gene	gene	NN
53	55	by	by	IN
56	63	vitamin	vitamin	NN
64	66	D3	d3	NN
66	67	:	:	:
68	74	direct	direct	JJ
75	85	inhibition	inhibition	NN
86	88	of	of	IN
89	99	NFATp/AP-1	nfatp/ap-1	NN
100	107	complex	complex	NN
108	117	formation	formation	NN
118	120	by	by	IN
121	122	a	a	DT
123	130	nuclear	nuclear	JJ
131	138	hormone	hormone	NN
139	147	receptor	receptor	NN
147	148	.	.	.

150	162	T-lymphocyte	T-lymphocyte	NNP
163	176	proliferation	proliferation	NN
177	179	is	be	VBZ
180	190	suppressed	suppress	VBN
191	193	by	by	IN
194	215	1,25-dihydroxyvitamin	1,25-dihydroxyvitamin	NN
216	218	D3	D3	NNP
219	220	[	[	(
220	231	1,25(OH)2D3	1,25(OH)2D3	NNP
231	232	]	]	)
232	233	,	,	,
234	237	the	the	DT
238	244	active	active	JJ
245	255	metabolite	metabolite	NN
256	258	of	of	IN
259	266	vitamin	vitamin	NN
267	269	D3	d3	NN
269	270	,	,	,
271	274	and	and	CC
275	277	is	be	VBZ
278	288	associated	associate	VBN
289	293	with	with	IN
294	295	a	a	DT
296	304	decrease	decrease	NN
305	307	in	in	IN
308	319	interleukin	interleukin	NN
320	321	2	2	CD
322	323	(	(	(
323	327	IL-2	il-2	NN
327	328	)	)	)
328	329	,	,	,
330	335	gamma	gamma	NN
336	346	interferon	interferon	NN
346	347	,	,	,
348	351	and	and	CC
352	374	granulocyte-macrophage	granulocyte-macrophage	JJ
375	393	colony-stimulating	colony-stimulating	JJ
394	400	factor	factor	NN
401	405	mRNA	mrna	NN
406	412	levels	level	NNS
412	413	.	.	.

414	416	We	we	PRP
417	423	report	report	VBP
424	428	here	here	RB
429	433	that	that	IN
434	454	1,25(OH)2D3-mediated	1,25(oh)2d3-mediated	JJ
455	465	repression	repression	NN
466	468	in	in	IN
469	475	Jurkat	Jurkat	NNP
476	481	cells	cell	NNS
482	484	is	be	VBZ
485	498	cycloheximide	cycloheximide	NN
499	508	resistant	resistant	JJ
508	509	,	,	,
510	520	suggesting	suggest	VBG
521	525	that	that	IN
526	528	it	it	PRP
529	531	is	be	VBZ
532	533	a	a	DT
534	540	direct	direct	JJ
541	556	transcriptional	transcriptional	JJ
557	567	repressive	repressive	JJ
568	574	effect	effect	NN
575	577	on	on	IN
578	582	IL-2	il-2	NN
583	593	expression	expression	NN
594	596	by	by	IN
597	600	the	the	DT
601	608	vitamin	vitamin	NN
609	611	D3	d3	NN
612	620	receptor	receptor	NN
621	622	(	(	(
622	625	VDR	VDR	NNP
625	626	)	)	)
626	627	.	.	.

628	630	We	we	PRP
631	640	therefore	therefore	RB
641	649	examined	examine	VBD
650	657	vitamin	vitamin	NN
658	669	D3-mediated	d3-mediated	JJ
670	680	repression	repression	NN
681	683	of	of	IN
684	693	activated	activate	VBN
694	698	IL-2	il-2	NN
699	709	expression	expression	NN
710	712	by	by	IN
713	727	cotransfecting	cotransfecte	VBG
728	734	Jurkat	jurkat	NN
735	740	cells	cell	NNS
741	745	with	with	IN
746	750	IL-2	il-2	NN
751	768	promoter/reporter	promoter/reporter	NN
769	779	constructs	construct	NNS
780	783	and	and	CC
784	785	a	a	DT
786	789	VDR	VDR	NNP
790	804	overexpression	overexpression	NN
805	811	vector	vector	NN
812	815	and	and	CC
816	818	by	by	IN
819	822	DNA	DNA	NNP
823	830	binding	binding	NN
830	831	.	.	.

832	834	We	we	PRP
835	845	delineated	delineate	VBD
846	848	an	an	DT
849	856	element	element	NN
857	867	conferring	confer	VBG
868	872	both	both	CC
873	876	DNA	dna	NN
877	884	binding	binding	NN
885	887	by	by	IN
888	891	the	the	DT
892	900	receptor	receptor	NN
901	903	in	in	FW
904	909	vitro	vitro	FW
910	913	and	and	CC
914	934	1,25(OH)2D3-mediated	1,25(oh)2d3-mediated	JJ
935	945	repression	repression	NN
946	948	in	in	FW
949	953	vivo	vivo	FW
954	956	to	to	TO
957	958	a	a	DT
959	964	short	short	JJ
965	970	40-bp	40-bp	JJ
971	977	region	region	NN
978	990	encompassing	encompass	VBG
991	993	an	an	DT
994	1003	important	important	JJ
1004	1012	positive	positive	JJ
1013	1023	regulatory	regulatory	JJ
1024	1031	element	element	NN
1031	1032	,	,	,
1033	1040	NF-AT-1	NF-AT-1	NNP
1040	1041	,	,	,
1042	1047	which	which	WDT
1048	1050	is	be	VBZ
1051	1056	bound	bind	VBN
1057	1059	by	by	IN
1060	1061	a	a	DT
1062	1077	T-cell-specific	t-cell-specific	JJ
1078	1091	transcription	transcription	NN
1092	1098	factor	factor	NN
1098	1099	,	,	,
1100	1105	NFATp	NFATp	NNP
1105	1106	,	,	,
1107	1109	as	as	RB
1110	1114	well	well	RB
1115	1117	as	as	IN
1118	1120	by	by	IN
1121	1125	AP-1	ap-1	NN
1125	1126	.	.	.

1127	1130	VDR	VDR	NNP
1131	1142	DNA-binding	dna-binding	JJ
1143	1150	mutants	mutant	NNS
1151	1155	were	be	VBD
1156	1162	unable	unable	JJ
1163	1165	to	to	TO
1166	1172	either	either	CC
1173	1177	bind	bind	VBP
1178	1180	to	to	TO
1181	1185	this	this	DT
1186	1193	element	element	NN
1194	1196	in	in	FW
1197	1202	vitro	vitro	FW
1203	1205	or	or	CC
1206	1213	repress	repress	VB
1214	1216	in	in	FW
1217	1221	vivo	vivo	FW
1221	1222	;	;	:
1223	1226	the	the	DT
1227	1230	VDR	VDR	NNP
1231	1242	DNA-binding	dna-binding	JJ
1243	1249	domain	domain	NN
1250	1255	alone	alone	RB
1255	1256	,	,	,
1257	1264	however	however	RB
1264	1265	,	,	,
1266	1271	bound	bind	VBD
1272	1275	the	the	DT
1276	1283	element	element	NN
1284	1287	but	but	CC
1288	1292	also	also	RB
1293	1298	could	could	MD
1299	1302	not	not	RB
1303	1310	repress	repress	VB
1311	1315	IL-2	il-2	NN
1316	1326	expression	expression	NN
1326	1327	.	.	.

1328	1333	These	these	DT
1334	1341	results	result	NNS
1342	1350	indicate	indicate	VBP
1351	1355	that	that	IN
1356	1359	DNA	dna	NN
1360	1367	binding	binding	NN
1368	1370	by	by	IN
1371	1374	VDR	VDR	NNP
1375	1377	is	be	VBZ
1378	1387	necessary	necessary	JJ
1388	1391	but	but	CC
1392	1395	not	not	RB
1396	1406	sufficient	sufficient	JJ
1407	1409	to	to	TO
1410	1417	mediate	mediate	VB
1418	1422	IL-2	il-2	NN
1423	1433	repression	repression	NN
1433	1434	.	.	.

1435	1437	By	by	IN
1438	1447	combining	combine	VBG
1448	1457	partially	partially	RB
1458	1466	purified	purify	VBN
1467	1475	proteins	protein	NNS
1476	1478	in	in	FW
1479	1484	vitro	vitro	FW
1484	1485	,	,	,
1486	1488	we	we	PRP
1489	1497	observed	observe	VBD
1498	1501	the	the	DT
1502	1506	loss	loss	NN
1507	1509	of	of	IN
1510	1513	the	the	DT
1514	1519	bound	bind	VBN
1520	1534	NFATp/AP-1-DNA	nfatp/ap-1-dna	NN
1535	1542	complex	complex	NN
1543	1547	upon	upon	IN
1548	1557	inclusion	inclusion	NN
1558	1560	of	of	IN
1561	1564	VDR	VDR	NNP
1565	1567	or	or	CC
1568	1580	VDR-retinoid	vdr-retinoid	JJ
1581	1582	X	X	NNP
1583	1591	receptor	receptor	NN
1591	1592	.	.	.

1593	1598	Order	Order	NNP
1599	1601	of	of	IN
1602	1610	addition	addition	NN
1611	1614	and	and	CC
1615	1623	off-rate	off-rate	JJ
1624	1635	experiments	experiment	NNS
1636	1644	indicate	indicate	VBP
1645	1649	that	that	IN
1650	1653	the	the	DT
1654	1666	VDR-retinoid	vdr-retinoid	JJ
1667	1668	X	X	NNP
1669	1677	receptor	receptor	NN
1678	1689	heterodimer	heterodimer	NN
1690	1696	blocks	block	VBZ
1697	1707	NFATp/AP-1	nfatp/ap-1	NN
1708	1715	complex	complex	NN
1716	1725	formation	formation	NN
1726	1729	and	and	CC
1730	1734	then	then	RB
1735	1741	stably	stably	RB
1742	1752	associates	associate	VBZ
1753	1757	with	with	IN
1758	1761	the	the	DT
1762	1769	NF-AT-1	nf-at-1	NN
1770	1777	element	element	NN
1777	1778	.	.	.

1779	1783	This	this	DT
1784	1790	direct	direct	JJ
1791	1801	inhibition	inhibition	NN
1802	1804	by	by	IN
1805	1806	a	a	DT
1807	1814	nuclear	nuclear	JJ
1815	1822	hormone	hormone	NN
1823	1831	receptor	receptor	NN
1832	1834	of	of	IN
1835	1850	transcriptional	transcriptional	JJ
1851	1861	activators	activator	NNS
1862	1864	of	of	IN
1865	1868	the	the	DT
1869	1873	IL-2	il-2	NN
1874	1878	gene	gene	NN
1879	1882	may	may	MD
1883	1890	provide	provide	VB
1891	1892	a	a	DT
1893	1904	mechanistic	mechanistic	JJ
1905	1916	explanation	explanation	NN
1917	1919	of	of	IN
1920	1923	how	how	WRB
1924	1931	vitamin	vitamin	NN
1932	1943	derivatives	derivative	NNS
1944	1947	can	can	MD
1948	1951	act	act	VB
1952	1954	as	as	IN
1955	1961	potent	potent	JJ
1962	1979	immunosuppressive	immunosuppressive	JJ
1980	1986	agents	agent	NNS
1986	1987	.	.	.